Evaluation of the Digene Hybrid Capture II Assay with the Rapid Capture System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae

DOI: 10.1128/JCM.40.10.3558-3564.2002

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FIG. 1.
(a) Comparison of initial results of HC2-RCS and HC2-M; (b) results of HC2-M and HC2-RCS for C. trachomatis; (c) HC2-M and HC2-RCS results for N. gonorrhoeae.

TABLE 1.

Comparison of HC2-RCS and HC2-M

TABLE 2.

Performance characteristics for detection of chlamydial infections

Assay	Analysis method	Sensitivity^a	Specificity^a	Positive predictive value (%)	Negative predictive value (%)
HC2-RCS CT-ID	Identification results alone analyzed for all samples	43/45 (95.6)	285/285 (100)	100	99.3
HC2-RCS CT-ID	Identification results considered only if CT/GC assay was positive	42/45 (93.3)	285/285 (100)	100	99.0
PCR		44/45 (97.8)	283/285 (99.3)	95.7	99.6
Culture		33/45 (73.3)	285/285 (100)	100	96.0

↵ a The data indicate number of positive samples/total number of samples tested (percent).

TABLE 3.

Performance characteristics for detection of gonococcal infections

Assay	Analysis method	Sensitivity^a	Specificity^a	Positive predictive value (%)	Negative predictive value (%)
HC2-RCS GC-ID	Identification results alone analyzed for all samples	21/21 (100)	306/309 (99.0)	87.5	100
HC2-RCS GC-ID	Identification results considered only if CT/GC assay was positive	21/21 (100)	307/309 (99.4)	91.3	100
PCR		21/21 (100)	306/309 (99.0)	87.5	100
Culture		19/21 (90.5)	309/309 (100)	100	99.4