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Bacteriology

Simple Microdilution Test for Detection of Metallo-β-Lactamase Production in Pseudomonas aeruginosa

Roberta Migliavacca, Jean-Denis Docquier, Claudia Mugnaioli, Gianfranco Amicosante, Rossana Daturi, Kyungwon Lee, Gian Maria Rossolini, Laura Pagani
Roberta Migliavacca
1Dipartimento di Scienze Morfologiche, Eidologiche e Cliniche, Sez. di Microbiologia, Università di Pavia
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Jean-Denis Docquier
2Dipartimento di Biologia Molecolare, Sez. di Microbiologia, Università di Siena, 53100 Siena
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Claudia Mugnaioli
2Dipartimento di Biologia Molecolare, Sez. di Microbiologia, Università di Siena, 53100 Siena
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Gianfranco Amicosante
3Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, 67100 L'Aquila, Italy
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Rossana Daturi
4Servizio di Analisi Microbiologiche, I.R.C.C.S. S. Matteo, 27100 Pavia
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Kyungwon Lee
5Department of Clinical Pathology and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea
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Gian Maria Rossolini
2Dipartimento di Biologia Molecolare, Sez. di Microbiologia, Università di Siena, 53100 Siena
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Laura Pagani
1Dipartimento di Scienze Morfologiche, Eidologiche e Cliniche, Sez. di Microbiologia, Università di Pavia
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  • For correspondence: lpagani@unipv.it
DOI: 10.1128/JCM.40.11.4388-4390.2002
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Tables

  • TABLE 1.

    Results of the EPI test carried out with 190 P. aeruginosa strains

    MBL production (no. of strains)MBL type (no. of strains)Phenotype (no. of strains)cMIC (μg/ml) ford:No. of strains showing a MIC reduction of:
    IMPcEPI>128-fold128-fold64-fold32-fold16-fold8-fold4-fold2-fold0
    Negative (168)Imps Cazs (2)a212
    Imps Cazr (5)2-41-414
    Impr-i Cazr-i (161)8-642-321410353
    Positive (22)bVIM-1 (7)Impr Cazr128-2560.5-16115
    VIM-2 (10)Impr Cazr32-5121-821421
    VIM-3 (1)Impr Cazr6421
    IMP-1 (1)Impr Cazr512161
    IMP-2 (3)Impr Cazr128-2568-32111
    • ↵ a Including the P. aeruginosa reference strains PAO-1 and ATCC27853.

    • ↵ b Including strains from references 3, 5, 6, and 15 to 17.

    • ↵ c Phenotypes listed indicate levels of susceptibility to the drugs imipenem (Imp) and ceftazidime (Caz). The superscript letters indicate the following: s, sensitivity, r, resistance; and r-i, resistance to intermediate susceptibility.

    • ↵ d Where a single value is not listed, the MIC range is given. IMP, imipenem; EPI, EDTA-phenanthroline-imipenem.

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Simple Microdilution Test for Detection of Metallo-β-Lactamase Production in Pseudomonas aeruginosa
Roberta Migliavacca, Jean-Denis Docquier, Claudia Mugnaioli, Gianfranco Amicosante, Rossana Daturi, Kyungwon Lee, Gian Maria Rossolini, Laura Pagani
Journal of Clinical Microbiology Nov 2002, 40 (11) 4388-4390; DOI: 10.1128/JCM.40.11.4388-4390.2002

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Simple Microdilution Test for Detection of Metallo-β-Lactamase Production in Pseudomonas aeruginosa
Roberta Migliavacca, Jean-Denis Docquier, Claudia Mugnaioli, Gianfranco Amicosante, Rossana Daturi, Kyungwon Lee, Gian Maria Rossolini, Laura Pagani
Journal of Clinical Microbiology Nov 2002, 40 (11) 4388-4390; DOI: 10.1128/JCM.40.11.4388-4390.2002
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  • Top
  • Article
    • ABSTRACT
    • Bacterial strains.
    • EPI microdilution test.
    • Detection of MBL producers by the EPI microdilution test.
    • Concluding remarks.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
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KEYWORDS

Anti-Bacterial Agents
imipenem
Pseudomonas aeruginosa
beta-lactamases

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