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Bacteriology

Typing of Neisseria gonorrhoeae Reveals Rapid Reinfection in Rural South Africa

Prashini Moodley, Iona M. C. Martin, Catherine A. Ison, A. Willem Sturm
Prashini Moodley
1Africa Centre for Health and Population Studies and the Department of Medical Microbiology, Nelson R. Mandela School of Medicine, University of Natal, Durban, South Africa
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Iona M. C. Martin
2Department of Infectious Diseases and Microbiology, Faculty of Medicine, Imperial College, London, United Kingdom
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Catherine A. Ison
2Department of Infectious Diseases and Microbiology, Faculty of Medicine, Imperial College, London, United Kingdom
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A. Willem Sturm
1Africa Centre for Health and Population Studies and the Department of Medical Microbiology, Nelson R. Mandela School of Medicine, University of Natal, Durban, South Africa
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  • For correspondence: sturm@nu.ac.za
DOI: 10.1128/JCM.40.12.4567-4570.2002
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    FIG. 1.

    Opa types of seven pairs (pre- and post-treatment isolates) of N. gonorrhoeae isolates. Double-headed arrows indicate identical opa types.

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  • TABLE 1.

    Sexual history of seven women culture positive for N. gonorrhoeae 10 days after ciprofloxacin treatment

    PatientAge (yr)Age at coitarche (yr)No. of lifetime sexual partnersRFaNo. of partners in last monthNo. of current partnersLocation of current partners(s)Condom use
    A301540.2511Local, separateNever
    B231730.511Local, togetherNever
    C21207711Local, separateNever
    D201520.422(i) 240 km away; (ii) local, separateOccasional
    E17162211250 km awayOccasional
    F211730.7511Local, separateNever
    G221630.51150 km awayNever
    • ↵ a RF = number of lifetime sexual partners/(age − age at coitarche).

  • TABLE 2.

    Drug MIC, presence of β-lactamase, and tetM genes of pre- and post-treatment isolates of N. gonorrhoeae

    PatientIsolate no.MIC (mg/liter) of penicillin/β-lactamaseMIC (mg/liter) of tetracycline/tetMMIC (mg/liter) of:
    SpectinomycinCeftriaxoneCiprofloxacinOfloxacin
    AA10.125/negative64/positive16≤0.007≤0.0070.03
    A20.25/negative4/negative8≤0.007≤0.0070.015
    BB11/negative128/positive16≤0.007≤0.0070.03
    B21/negative128/positive16≤0.0070.1252
    CC10.5/negative32/positive16≤0.007≤0.007≤0.007
    C20.5/negative16/positive16≤0.007≤0.0070.015
    DD18/positive32/positive16≤0.007≤0.007≤0.007
    D24/positive32/positive16≤0.007≤0.0070.015
    EE10.5/negative2/negative16≤0.007≤0.0070.015
    E21/negative1/negative16≤0.007≤0.0070.03
    FF10.25/negative32/positive32≤0.007≤0.0070.015
    F20.25/negative32/positive16≤0.007≤0.0070.015
    GG10.25/negative16/positive16≤0.007≤0.007≤0.007
    G20.25/negative32/positive16≤0.007≤0.0070.015
  • TABLE 3.

    Comparison of pre- and post-treatment N. gonorrhoeae isolates from seven women by four typing methods

    PatientIsolate no.Result of typing system:Identical isolates
    AntibiogramAuxotypeaSerotypeopa type
    AA1DifferentNRIA-6DifferentNo
    A2PROIB-2
    BB1DifferentARGIA-6DifferentNo
    B2NRIB-7
    CC1SameNRIB-8DifferentNo
    C2NRIB-3
    DD1SameNRA-NTSameYes
    D2NRA-NT
    EE1SamePROIB-3DifferentNo
    E2PROIB-3
    FF1SameNRIB-3DifferentNo
    F2NRIB-6
    GG1SameNRIB-3DifferentNo
    G2NRIA-6
    • ↵ a NR, not requiring; PRO, proline requiring; ARG, arginine requiring.

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Typing of Neisseria gonorrhoeae Reveals Rapid Reinfection in Rural South Africa
Prashini Moodley, Iona M. C. Martin, Catherine A. Ison, A. Willem Sturm
Journal of Clinical Microbiology Dec 2002, 40 (12) 4567-4570; DOI: 10.1128/JCM.40.12.4567-4570.2002

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Typing of Neisseria gonorrhoeae Reveals Rapid Reinfection in Rural South Africa
Prashini Moodley, Iona M. C. Martin, Catherine A. Ison, A. Willem Sturm
Journal of Clinical Microbiology Dec 2002, 40 (12) 4567-4570; DOI: 10.1128/JCM.40.12.4567-4570.2002
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KEYWORDS

Bacterial Typing Techniques
Genital Diseases, Female
gonorrhea
Neisseria gonorrhoeae
Rural Population

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