Mycobacterium avium subsp. paratuberculosis Strains from Cattle and Sheep Can Be Distinguished by a PCR Test Based on a Novel DNA Sequence Difference

DOI: 10.1128/JCM.40.12.4760-4762.2002

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FIG. 1.
Alignment of homologous DNA sequences from cattle and sheep types of M. paratuberculosis. Nucleotides identical in the two sequences are shown in reverse type, arrows indicate the identities and directions of the oligonucleotide primers used, the tandem DNA sequence present in the sheep type is shown in boxes, the palindromic region is underlined, and the coding sequence of the putative gene involved in phage attachment is shown in lowercase letters.
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FIG. 2.
PCR products from cattle and sheep types of M. paratuberculosis amplified with DMC529, DMC531, and DMC533. Lanes: 1 and 11, molecular size markers; 2 to 5, cattle types; 6 to 9, sheep types; 10, negative control.

TABLE 1.

Strains of the MAI complex and M. tuberculosis complex subjected to PCR

No. of strains	No. of strains, with insertion sequence:		Description	Source^a
No. of strains	IS900	IS901	Description	Source^a
10	0	3	Reference serotypes 1 to 6 and 8 to 11 of MAI complex^b	D. Dawson (16)
11	0	7	Cattle, deer, and pig isolates of MAI complex^b	New Zealand
6	0	5	Bird isolates of MAI complex^b	New Zealand
4	0	0	Human isolates of MAI complex^b	New Zealand
14	14		M. paratuberculosis cattle type	New Zealand
3	3		M. paratuberculosis cattle type	Canada
1	1		M. paratuberculosis TMC1613; cattle type	USA
1	1		M. paratuberculosis 316F; cattle type	UK
7	7		M. paratuberculosis sheep type	New Zealand
1	1		M. paratuberculosis sheep type	Canada
3	3		M. paratuberculosis sheep type	South Africa
1	1		M. paratuberculosis sheep type	Iceland
1			M. tuberculosis H37Rv	USA
2			M. bovis	New Zealand