Article Figures & Data
Figures
- FIG. 1.
PFGE patterns generated after SmaI macrorestriction of S. suis genome. Lanes 1 and 10, DNA molecular size marker; lanes 2 and 3, patterns 55 and 11 presenting 5 and 12 bands, respectively; lanes 4 and 5 correspond to strains isolated from healthy carrier pigs and belonging to patterns P23 and P73, respectively; lanes 6, 7, and 8, patterns P57, P59, and P60, respectively, corresponding to S. suis strains isolated from humans; lane 9 corresponds to S. suis strain belonging to the pattern P62.
- FIG.2.
Genetic relationships between 123 S. suis strains, as estimated by clustering analysis of PFGE patterns, was obtained after macrorestriction with SmaI. The classification and divergence of strains were calculated by the unweighted pair group method with arithmetic mean, and a confidence interval of 7.5% was used. The species where the strains have been isolated, capsular types, origins, and numbers of strains for each PFGE patterns are reported in the dendrogram. Footnote-style, superscript letters indicate that the abbreviations in the corresponding columns are defined here, as follows. (a) P, pig; H, human. (b) AUT, autoagglutinable. (c) S, septicemia; PT, palatine tonsils; NC, nasal cavities; A, arthritis; M, meningitis; nd, not done; ref, reference strain.
Tables
- TABLE 1.
Distribution of PFGE patterns and groups in relation to capsular types and origins of S. suis strains
Parameter na Value for group S. suis isolates Capsular type Origin Virulentb Nonvirulentc Otherd 2 1/2 3 7 9 AUTe Human Pig No. of isolates 123 90 30 3 77 13 7 14 10 2 26 97 No. of PFGE patterns 74 55 23 3 39 9 6 14 8 2 13 66 No. in PFGE groupf A a 2 1 1 0 1 1 0 0 0 0 0 2 b 40 25 14 1 22 6 2 7 1 2 5 35 c 10 5 5 0 5 3 0 2 0 0 0 10 B d 26 23 2 1 15 2 0 1 8 0 5 21 e 35 29 5 1 28 0 5 1 1 0 14 21 f 2 1 1 0 0 1 0 1 0 0 0 2 C g 6 6 0 0 6 0 0 0 0 0 2 4 h 2 0 2 0 0 0 0 2 0 0 0 2 ↵ a n, total no.
↵ b S. suis strains isolated from meningitis, arthritis, and septicemia in pigs and humans were classified as virulent strains.
↵ c Strains isolated from palatine tonsils and nasal cavities of clinically healthy pigs were classified as avirulent strains.
↵ d Isolation of three strains was not done.
↵ e AUT, autoagglutinable strains of S. suis.
↵ f The 123 isolates of S. suis diverged, and at 60% homology, three PFGE groups, A, B, and C, were identified, and at 69% homology, eight subgroups, a to h, were observed.
