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Journal of Clinical Microbiology
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Virology

Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal

Carmen Aldea, Carmen P. Alvarez, Lola Folgueira, Rafael Delgado, Joaquín R. Otero
Carmen Aldea
Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain
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Carmen P. Alvarez
Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain
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Lola Folgueira
Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain
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Rafael Delgado
Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain
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Joaquín R. Otero
Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain
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  • For correspondence: jrotero@hdoc.insalud.es
DOI: 10.1128/JCM.40.3.1060-1062.2002
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ABSTRACT

We have evaluated a real-time PCR procedure based on the LightCycler technology for rapid detection of herpes simplex virus (HSV) in genital lesions. Two sets of primers, corresponding to the thymidine kinase and DNA polymerase regions, were used for the amplification reactions in separate capillaries containing the SYBR Green I dye as detection signal. In 28 of 118 samples (24%), HSV was isolated by conventional cell culture. All cell culture-positive samples were also positive by real-time PCR. Six additional cell culture-negative samples were positive by PCR with both sets of primers. Total processing time was less than 3 h. Real-time PCR using SYBR Green I as detection signal is a sensitive procedure for the rapid diagnosis of HSV in genital lesions.

  • Copyright © 2002 American Society for Microbiology
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Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal
Carmen Aldea, Carmen P. Alvarez, Lola Folgueira, Rafael Delgado, Joaquín R. Otero
Journal of Clinical Microbiology Mar 2002, 40 (3) 1060-1062; DOI: 10.1128/JCM.40.3.1060-1062.2002

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Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal
Carmen Aldea, Carmen P. Alvarez, Lola Folgueira, Rafael Delgado, Joaquín R. Otero
Journal of Clinical Microbiology Mar 2002, 40 (3) 1060-1062; DOI: 10.1128/JCM.40.3.1060-1062.2002
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KEYWORDS

DNA, Viral
Genital Diseases, Female
Genital Diseases, Male
Organic Chemicals
polymerase chain reaction
Simplexvirus
Ulcer

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