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Journal of Clinical Microbiology
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Virology

Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal

Carmen Aldea, Carmen P. Alvarez, Lola Folgueira, Rafael Delgado, Joaquín R. Otero
Carmen Aldea
Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain
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Carmen P. Alvarez
Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain
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Lola Folgueira
Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain
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Rafael Delgado
Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain
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Joaquín R. Otero
Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain
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  • For correspondence: jrotero@hdoc.insalud.es
DOI: 10.1128/JCM.40.3.1060-1062.2002
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    FIG. 1.

    Performance of real-time PCR on a panel of control samples with quantified number of copies of HSV DNA. The graphic shows the evolution of the fluorescent signal related to cycle number on a panel of quantified copies of a plasmid containing the Tk fragment of an HSV-1 isolate. Similar results were obtained with the DNA Pol plasmid panel.

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    FIG. 2.

    Melting curves of the amplified fragments generated by real-time PCR. These melting curves were generated on clinical samples with primers for Tk. Specific signals had melting temperatures ranging from 93 to 95°C. Similar results were obtained with the Pol set of primers. HSV-1 and HSV-2 amplification products were clearly differentiated from unspecific amplification (primer dimmers), exhibiting melting temperatures below 85°C.

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Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal
Carmen Aldea, Carmen P. Alvarez, Lola Folgueira, Rafael Delgado, Joaquín R. Otero
Journal of Clinical Microbiology Mar 2002, 40 (3) 1060-1062; DOI: 10.1128/JCM.40.3.1060-1062.2002

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Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal
Carmen Aldea, Carmen P. Alvarez, Lola Folgueira, Rafael Delgado, Joaquín R. Otero
Journal of Clinical Microbiology Mar 2002, 40 (3) 1060-1062; DOI: 10.1128/JCM.40.3.1060-1062.2002
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KEYWORDS

DNA, Viral
Genital Diseases, Female
Genital Diseases, Male
Organic Chemicals
polymerase chain reaction
Simplexvirus
Ulcer

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