Article Figures & Data
Figures
- FIG. 1.
Performance of real-time PCR on a panel of control samples with quantified number of copies of HSV DNA. The graphic shows the evolution of the fluorescent signal related to cycle number on a panel of quantified copies of a plasmid containing the Tk fragment of an HSV-1 isolate. Similar results were obtained with the DNA Pol plasmid panel.
- FIG. 2.
Melting curves of the amplified fragments generated by real-time PCR. These melting curves were generated on clinical samples with primers for Tk. Specific signals had melting temperatures ranging from 93 to 95°C. Similar results were obtained with the Pol set of primers. HSV-1 and HSV-2 amplification products were clearly differentiated from unspecific amplification (primer dimmers), exhibiting melting temperatures below 85°C.
