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Bacteriology

Characterization of Shiga Toxin-Producing Escherichia coli O26 Strains and Establishment of Selective Isolation Media for These Strains

Reiji Hiramatsu, Masakado Matsumoto, Yoshio Miwa, Yasumoto Suzuki, Makoto Saito, Yutaka Miyazaki
Reiji Hiramatsu
Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan
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Masakado Matsumoto
Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan
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Yoshio Miwa
Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan
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Yasumoto Suzuki
Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan
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Makoto Saito
Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan
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Yutaka Miyazaki
Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan
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  • For correspondence: yutaka_miyazaki@mail.pref.aichi.jp
DOI: 10.1128/JCM.40.3.922-925.2002
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Tables

  • TABLE 1.

    Fermentation of 15 selected carbohydrates by STEC O26, O157, and O111 and by non-STECa

    CarbohydrateNo. of STEC strainsNo. of non-STEC strains (n = 59)
    O26 (n = 31)O157 (n = 100)O111 (n = 8)
    Rhamnose0100858
    Arabinose31100859
    Dulcitol099847
    Glucose31100859
    Inositol0002
    Lactose31100853
    Maltose31100758
    Mannitol31100859
    Mannose31100859
    Raffinose30100845
    Salicin310033
    Sorbitol300852
    Sucrose3196742
    Trehalose31100857
    Xylose31100858
    • ↵ a n, number of strains examined.

  • TABLE 2.

    Percentages of the number of colonies generated on RMAC and CT-RMAC compared to those generated on DHL

    Isolate no.Serotype% Colonies (versus DHL)No. of colonies on DHL
    RMACaCT-RMACb
    1O26:H1111410051
    2O26:H1110084100
    3O26:H111584840
    4O26:H1114210450
    5O26:H1119311845
    6O26:H1135511331
    7O26:H119270120
    8O26:H111509020
    9O26:H1115610648
    10O26:H111148851
    11O26:H119690125
    12O26:H111947232
    13O26:H11934770
    14O26:H118957148
    15O26:H111079395
    16O26:H1111190100
    17O26:H117446125
    18O26:H111244555
    19O26:H111209790
    20O26:H118510772
    21O26:H1117211495
    22O26:H11138102139
    23O26:H1116596112
    24O26:H11125103208
    25O26:H−9513040
    26O26:H−1133938
    27O26:H−11110190
    28O26:H−20312840
    29O26:H−1988946
    30O26:H−25612425
    31O26:H−7772130
    • ↵ a Median = 120; mean ± standard deviation = 139 ± 59.

    • ↵ b Median = 93; mean ± standard deviation = 89 ± 26.

  • TABLE 3.

    Growth and color of colonies of STEC O26 and selected enteric bacteria on RMAC and CT-RMAC

    OrganismSerotypeNo. of strains examinedNo. of colonies on:
    RMACCT-RMAC
    RedColorlessRedColorless
    STECO2631031031
    O15726260260
    O11188080
    Non-STECO1571313030
    O26129350
    Other3433110
    Non-Escherichia coli
    Enterobacter cloacae 54101
    Salmonella enterica serovar Enteritidis55000
    Shigella flexneri 50502
    Shigella sonnei 51400
  • TABLE 4.

    Detection limit of STEC O26 in stool samples

    Isolate no.MediumNo. of colonies identified as STEC O26/total no. of coloniesa with an inoculumb of:
    101102103104
    1RMAC0/50/52/55/5
    CT-RMACNG2/25/55/5
    DHLNT0/50/50/5
    2RMAC0/50/53/54/5
    CT-RMACNG1/15/55/5
    DHLNT0/50/50/5
    3RMAC0/51/53/55/5
    CT-RMACNG2/25/55/5
    DHLNT0/50/50/5
    4RMAC0/50/51/54/5
    CT-RMACNGNG5/55/5
    DHLNT0/50/50/5
    5RMAC0/50/51/54/5
    CT-RMACNGNG5/55/5
    DHLNT0/50/50/5
    • ↵ a NG, no growth; NT, not tested.

    • ↵ b The inoculum sizes were 101, 102, 103, and 104 seeded organisms/ml of stool sample, as indicated.

  • TABLE 5.

    Number of STEC O26 colonies from milk samples on RMAC and CT-RMAC

    Isolate no.MediumNo. of STEC O26 colonies with an inoculuma of:
    100101102103
    1RMAC0210103
    CT-RMAC02873
    2RMAC0324212
    CT-RMAC0226180
    3RMAC0122187
    CT-RMAC0117137
    4RMAC0628233
    CT-RMAC0624190
    5RMAC0211150
    CT-RMAC00399
    • ↵ a The inoculum sizes were 100, 101, 102, and 103 seeded organisms/ml of milk, as indicated.

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Characterization of Shiga Toxin-Producing Escherichia coli O26 Strains and Establishment of Selective Isolation Media for These Strains
Reiji Hiramatsu, Masakado Matsumoto, Yoshio Miwa, Yasumoto Suzuki, Makoto Saito, Yutaka Miyazaki
Journal of Clinical Microbiology Mar 2002, 40 (3) 922-925; DOI: 10.1128/JCM.40.3.922-925.2002

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Characterization of Shiga Toxin-Producing Escherichia coli O26 Strains and Establishment of Selective Isolation Media for These Strains
Reiji Hiramatsu, Masakado Matsumoto, Yoshio Miwa, Yasumoto Suzuki, Makoto Saito, Yutaka Miyazaki
Journal of Clinical Microbiology Mar 2002, 40 (3) 922-925; DOI: 10.1128/JCM.40.3.922-925.2002
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KEYWORDS

Carbohydrate Metabolism
Escherichia coli
Fermentation
Shiga toxin

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