DOI: 10.1128/JCM.40.3.922-925.2002
Article Figures & Data
Tables
- TABLE 1.
Fermentation of 15 selected carbohydrates by STEC O26, O157, and O111 and by non-STECa
Carbohydrate No. of STEC strains No. of non-STEC strains (n = 59) O26 (n = 31) O157 (n = 100) O111 (n = 8) Rhamnose 0 100 8 58 Arabinose 31 100 8 59 Dulcitol 0 99 8 47 Glucose 31 100 8 59 Inositol 0 0 0 2 Lactose 31 100 8 53 Maltose 31 100 7 58 Mannitol 31 100 8 59 Mannose 31 100 8 59 Raffinose 30 100 8 45 Salicin 31 0 0 33 Sorbitol 30 0 8 52 Sucrose 31 96 7 42 Trehalose 31 100 8 57 Xylose 31 100 8 58 ↵ a n, number of strains examined.
- TABLE 2.
Percentages of the number of colonies generated on RMAC and CT-RMAC compared to those generated on DHL
Isolate no. Serotype % Colonies (versus DHL) No. of colonies on DHL RMACa CT-RMACb 1 O26:H11 114 100 51 2 O26:H11 100 84 100 3 O26:H11 158 48 40 4 O26:H11 142 104 50 5 O26:H11 193 118 45 6 O26:H11 355 113 31 7 O26:H11 92 70 120 8 O26:H11 150 90 20 9 O26:H11 156 106 48 10 O26:H11 114 88 51 11 O26:H11 96 90 125 12 O26:H11 194 72 32 13 O26:H11 93 47 70 14 O26:H11 89 57 148 15 O26:H11 107 93 95 16 O26:H11 111 90 100 17 O26:H11 74 46 125 18 O26:H11 124 45 55 19 O26:H11 120 97 90 20 O26:H11 85 107 72 21 O26:H11 172 114 95 22 O26:H11 138 102 139 23 O26:H11 165 96 112 24 O26:H11 125 103 208 25 O26:H− 95 130 40 26 O26:H− 113 39 38 27 O26:H− 111 101 90 28 O26:H− 203 128 40 29 O26:H− 198 89 46 30 O26:H− 256 124 25 31 O26:H− 77 72 130 - TABLE 3.
Growth and color of colonies of STEC O26 and selected enteric bacteria on RMAC and CT-RMAC
Organism Serotype No. of strains examined No. of colonies on: RMAC CT-RMAC Red Colorless Red Colorless STEC O26 31 0 31 0 31 O157 26 26 0 26 0 O111 8 8 0 8 0 Non-STEC O157 13 13 0 3 0 O26 12 9 3 5 0 Other 34 33 1 1 0 Non-Escherichia coli Enterobacter cloacae 5 4 1 0 1 Salmonella enterica serovar Enteritidis 5 5 0 0 0 Shigella flexneri 5 0 5 0 2 Shigella sonnei 5 1 4 0 0 - TABLE 4.
Detection limit of STEC O26 in stool samples
Isolate no. Medium No. of colonies identified as STEC O26/total no. of coloniesa with an inoculumb of: 101 102 103 104 1 RMAC 0/5 0/5 2/5 5/5 CT-RMAC NG 2/2 5/5 5/5 DHL NT 0/5 0/5 0/5 2 RMAC 0/5 0/5 3/5 4/5 CT-RMAC NG 1/1 5/5 5/5 DHL NT 0/5 0/5 0/5 3 RMAC 0/5 1/5 3/5 5/5 CT-RMAC NG 2/2 5/5 5/5 DHL NT 0/5 0/5 0/5 4 RMAC 0/5 0/5 1/5 4/5 CT-RMAC NG NG 5/5 5/5 DHL NT 0/5 0/5 0/5 5 RMAC 0/5 0/5 1/5 4/5 CT-RMAC NG NG 5/5 5/5 DHL NT 0/5 0/5 0/5 - TABLE 5.
Number of STEC O26 colonies from milk samples on RMAC and CT-RMAC
Isolate no. Medium No. of STEC O26 colonies with an inoculuma of: 100 101 102 103 1 RMAC 0 2 10 103 CT-RMAC 0 2 8 73 2 RMAC 0 3 24 212 CT-RMAC 0 2 26 180 3 RMAC 0 1 22 187 CT-RMAC 0 1 17 137 4 RMAC 0 6 28 233 CT-RMAC 0 6 24 190 5 RMAC 0 2 11 150 CT-RMAC 0 0 3 99 ↵ a The inoculum sizes were 100, 101, 102, and 103 seeded organisms/ml of milk, as indicated.
