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Bacteriology

Six-Year Molecular Analysis of Burkholderia cepacia Complex Isolates among Cystic Fibrosis Patients at a Referral Center for Lung Transplantation

David G. Heath, Kathy Hohneker, Charlene Carriker, Kelly Smith, Jonathan Routh, John J. LiPuma, Robert M. Aris, David Weber, Peter H. Gilligan
David G. Heath
1Clinical Microbiology-Immunology Laboratories
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Kathy Hohneker
2 Medicine
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Charlene Carriker
3Department of Hospital Epidemiology, University of North Carolina Hospitals
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Kelly Smith
1Clinical Microbiology-Immunology Laboratories
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Jonathan Routh
1Clinical Microbiology-Immunology Laboratories
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John J. LiPuma
4Department of Pediatrics and Communicable Diseases, The University of Michigan, Ann Arbor, Michigan
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Robert M. Aris
2 Medicine
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David Weber
2 Medicine
3Department of Hospital Epidemiology, University of North Carolina Hospitals
5Pediatrics, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina
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Peter H. Gilligan
1Clinical Microbiology-Immunology Laboratories
6Microbiology-Immunology
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  • For correspondence: pgilliga@unch.unc.edu
DOI: 10.1128/JCM.40.4.1188-1193.2002
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    FIG. 1.

    PFGE banding patterns of six clusters (clusters A to F) of B. cepacia complex isolates recovered from CF patients at the UNC Hospitals CF center. The cluster C gel contains a B. multivorans strain designated 23 for purposes of comparison. The cluster E gel contains the Toronto strain, designated ET, and a cblA+ genomovar III strain, designated 21, for the purposes of comparison. Numbers on the left are molecular weights.

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    FIG. 2.

    Time line graph showing the hospitalization course of patients (Pt.) 11 to 14. Horizontal bars represent inpatient status, with the admission date given at the beginning of the bars and the discharge date given at the ends. Vertical bars represent dates of concurrent admission resulting in possible cross infection. #, +, and ∗, the dates that patients 12, 13, and 14, respectively, first became positive for B. multivorans infection (see text).

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  • TABLE 1.

    Summary of genotypes and clusters of B. cepacia complex isolates recovered from January 1995 to March 2001

    GenotypePatient no.GenomovarClusterAreacblAa
    11IIIAMichiganNeg
    12IIIAMichiganNeg
    13IIIAMichiganNeg
    1a4IIIAMichiganNeg
    15IIIANebraskaNeg
    1b6IIIAMichiganNeg
    27IIIBNew YorkNeg
    28IIIBNew YorkNeg
    29IIIBNew YorkNeg
    210IIIBNew JerseyNeg
    311 B. multivorans (II)CFloridaNeg
    312 B. multivorans (II)CUNC HospitalsNeg
    313 B. multivorans (II)CUNC HospitalsNeg
    314 B. multivorans (II)CUNC HospitalsNeg
    415IIIDUNC HospitalsNeg
    416IIIDUNC HospitalsNeg
    517IIIENova ScotiaPos
    518IIIENova ScotiaPos
    619IIIFUNC HospitalsNeg
    620IIIFNew JerseyNeg
    721IIIDistinct genotypePos
    822 B. cepacia (I)Distinct genotypePos
    9-3023-44 B. multivorans (II)Distinct genotypesNeg
    31-3845-52IIIDistinct genotypesNeg
    39-4153-55 B. vietnamiensis (V)Distinct genotypesNeg
    4256 B. cepacia (I)Distinct genotypeNeg
    • ↵ a Neg, negative; Pos, positive.

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Six-Year Molecular Analysis of Burkholderia cepacia Complex Isolates among Cystic Fibrosis Patients at a Referral Center for Lung Transplantation
David G. Heath, Kathy Hohneker, Charlene Carriker, Kelly Smith, Jonathan Routh, John J. LiPuma, Robert M. Aris, David Weber, Peter H. Gilligan
Journal of Clinical Microbiology Apr 2002, 40 (4) 1188-1193; DOI: 10.1128/JCM.40.4.1188-1193.2002

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Six-Year Molecular Analysis of Burkholderia cepacia Complex Isolates among Cystic Fibrosis Patients at a Referral Center for Lung Transplantation
David G. Heath, Kathy Hohneker, Charlene Carriker, Kelly Smith, Jonathan Routh, John J. LiPuma, Robert M. Aris, David Weber, Peter H. Gilligan
Journal of Clinical Microbiology Apr 2002, 40 (4) 1188-1193; DOI: 10.1128/JCM.40.4.1188-1193.2002
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KEYWORDS

Burkholderia Infections
Burkholderia cepacia
cystic fibrosis
lung transplantation
Referral and Consultation

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