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Chlamydiology and Rickettsiology

PreservCyt Transport Medium Used for the ThinPrep Pap Test Is a Suitable Medium for Detection of Chlamydia trachomatis by the COBAS Amplicor CT/NG Test: Results of a Preliminary Study and Future Implications

Anne Bianchi, François Moret, Jean-Marc Desrues, Thierry Champenois, Yves Dervaux, Orlane Desvouas, André Oursin, Dominique Quinzat, Roger Dachez, Christian Bathelier, Christophe Ronsin
Anne Bianchi
1Laboratoire Départemental de Seine-Saint-Denis, Conseil Général, Bondy
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François Moret
2Roche Diagnostics, Meylan
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Jean-Marc Desrues
3LCL Service de Biologie Moléculaire, Ivry sur Seine
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Thierry Champenois
3LCL Service de Biologie Moléculaire, Ivry sur Seine
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Yves Dervaux
4Cytyc France SARL, Villepinte,
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Orlane Desvouas
3LCL Service de Biologie Moléculaire, Ivry sur Seine
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André Oursin
3LCL Service de Biologie Moléculaire, Ivry sur Seine
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Dominique Quinzat
5Laboratoire Claude Lévy, Paris, France
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Roger Dachez
5Laboratoire Claude Lévy, Paris, France
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Christian Bathelier
3LCL Service de Biologie Moléculaire, Ivry sur Seine
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Christophe Ronsin
3LCL Service de Biologie Moléculaire, Ivry sur Seine
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  • For correspondence: c.ronsin@lablcl.com
DOI: 10.1128/JCM.40.5.1749-1754.2002
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Tables

  • TABLE 1.

    Comparison of the sensitivities of 2SP and PreservCyt (ThinPrep Pap test) for detection of C. trachomatis by the COBAS Amplicor CT/NG test

    Batch and mediumA660 of the following dilution (IFU/ml)a:
    1031021010.10.01
    C. trachomatisICC. trachomatisICC. trachomatisICC. trachomatisICC. trachomatisICC. trachomatisIC
    Batch 1
        2SP 3.24 3.84 3.54 3.99 3.99 3.99 3.99 3.99 2.03 3.99 0.0033.99
    3.21 3.99 3.62 3.99 3.85 3.99 3.99 3.99 0.0073.990.0033.99
        PreserCyt 3.17 3.84 3.72 3.99 3.99 3.99 3.42 3.99 0.0013.990.0013.99
    3.28 3.48 3.84 3.84 3.99 1.00 3.99 0.0023.990.0023.99
    Batch 2
        2SP 3.30 3.99 3.78 3.99 3.99 3.99 0.0023.990.0023.990.0023.76
    3.23 3.99 3.60 3.77 3.99 3.77 3.12 3.77 0.0023.770.0023.77
        PreserCyt 3.42 3.99 3.99 3.99 3.42 3.99 0.0023.990.0023.990.0033.99
    3.62 3.99 3.84 3.99 3.99 3.99 0.0033.990.0023.990.0023.93
    Batch 3
        2SP 3.19 3.99 3.63 3.99 3.63 3.93 3.93 3.93 1.99 3.76 0.0023.76
    3.19 3.93 3.63 3.63 3.93 3.76 2.36 3.93 0.0013.760.0013.76
        PreserCyt 3.62 3.99 3.99 3.99 3.80 3.99 0.0023.990.0013.990.0013.99
    3.62 3.99 3.80 3.99 2.19 3.79 0.0023.770.0013.770.0033.77
    Batch 4
        2SP 3.33 3.93 3.63 3.63 3.75 3.93 2.25 3.76 3.45 3.76 0.0023.76
    3.33 3.93 3.39 3.93 3.75 3.76 3.99 3.63 0.0023.760.0023.76
        PreserCyt 3.62 3.99 3.79 3.79 3.99 3.99 0.0033.990.0023.790.0023.79
    3.49 3.99 3.99 3.79 3.79 3.79 0.0033.990.0023.790.0033.79
    • ↵ a Results for duplicate assays are shown. Positive results are boldfaced..

  • TABLE 2.

    Performance of COBAS Amplicor CT/NG test for detection of C. trachomatis in ThinPrep Pap test samplesa

    Age (yr) of female population (no. of persons)bNo. of PCR resultsSpecimens with inhibition
    PositiveNegative
    15 (n = 5)14None
    16 (n = 13)13None
    17 (n = 31)130None
    18 (n = 67)67None
    19 (n = 76)76None
    20 (n = 130)5125None
    21 (n = 132)4128None
    22 (n = 117)4113None
    23 (n = 150)2148None
    24 (n = 124)3121None
    25 (n = 155)2153None
        Total (n = 1,000)22978None
    • ↵ a None of the women tested showed clinical signs of urogenital infection, and cytological results were normal for all specimens.\

    • ↵ b C. trachomatis PCR results were positive for 2 of the 116 15- to 18-year-olds tested (prevalence, 1.7%) and for 20 of the 884 19- to 25-year-olds tested (prevalence, 2.3%).

  • TABLE 3.

    Impact of storage conditions on the performance of the COBAS Amplicor CT/NG test

    Sample no.C. trachomatis A660 at:
    Day 0Day 15Day 30Day 45
    1a3.043.053.052.73
    2a3.082.953.012.77
    3a3.163.372.953.48
    4>4>4>42.76
    52.972.952.471.95
    61.973.133.373.10
    7a3.293.293.423.43
    8a3.033.332.262.98
    93.373.723.733.73
    10a3.723.553.333.21
    113.853.733.433.74
    123.283.102.642.77
    133.213.333.133.13
    14a>43.26NDb3.56
    153.183.113.643.16
    162.783.86ND3.39
    17a3.083.643.113.19
    18a2.783.513.262.83
    193.093.29ND1.94
    203.443.03ND2.15
    212.69NDND2.69
    223.06NDND1.72
    • ↵ a For this sample, a second independent PCR test was performed on an endocervical swab specimen collected in 2SP.\

    • ↵ b ND, not determined.

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PreservCyt Transport Medium Used for the ThinPrep Pap Test Is a Suitable Medium for Detection of Chlamydia trachomatis by the COBAS Amplicor CT/NG Test: Results of a Preliminary Study and Future Implications
Anne Bianchi, François Moret, Jean-Marc Desrues, Thierry Champenois, Yves Dervaux, Orlane Desvouas, André Oursin, Dominique Quinzat, Roger Dachez, Christian Bathelier, Christophe Ronsin
Journal of Clinical Microbiology May 2002, 40 (5) 1749-1754; DOI: 10.1128/JCM.40.5.1749-1754.2002

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PreservCyt Transport Medium Used for the ThinPrep Pap Test Is a Suitable Medium for Detection of Chlamydia trachomatis by the COBAS Amplicor CT/NG Test: Results of a Preliminary Study and Future Implications
Anne Bianchi, François Moret, Jean-Marc Desrues, Thierry Champenois, Yves Dervaux, Orlane Desvouas, André Oursin, Dominique Quinzat, Roger Dachez, Christian Bathelier, Christophe Ronsin
Journal of Clinical Microbiology May 2002, 40 (5) 1749-1754; DOI: 10.1128/JCM.40.5.1749-1754.2002
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KEYWORDS

Chlamydia Infections
Chlamydia trachomatis

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