DOI: 10.1128/JCM.40.5.1749-1754.2002
Article Figures & Data
Tables
- TABLE 1.
Comparison of the sensitivities of 2SP and PreservCyt (ThinPrep Pap test) for detection of C. trachomatis by the COBAS Amplicor CT/NG test
Batch and medium A660 of the following dilution (IFU/ml)a: 103 102 10 1 0.1 0.01 C. trachomatis IC C. trachomatis IC C. trachomatis IC C. trachomatis IC C. trachomatis IC C. trachomatis IC Batch 1 2SP 3.24 3.84 3.54 3.99 3.99 3.99 3.99 3.99 2.03 3.99 0.003 3.99 3.21 3.99 3.62 3.99 3.85 3.99 3.99 3.99 0.007 3.99 0.003 3.99 PreserCyt 3.17 3.84 3.72 3.99 3.99 3.99 3.42 3.99 0.001 3.99 0.001 3.99 3.28 3.48 3.84 3.84 3.99 1.00 3.99 0.002 3.99 0.002 3.99 Batch 2 2SP 3.30 3.99 3.78 3.99 3.99 3.99 0.002 3.99 0.002 3.99 0.002 3.76 3.23 3.99 3.60 3.77 3.99 3.77 3.12 3.77 0.002 3.77 0.002 3.77 PreserCyt 3.42 3.99 3.99 3.99 3.42 3.99 0.002 3.99 0.002 3.99 0.003 3.99 3.62 3.99 3.84 3.99 3.99 3.99 0.003 3.99 0.002 3.99 0.002 3.93 Batch 3 2SP 3.19 3.99 3.63 3.99 3.63 3.93 3.93 3.93 1.99 3.76 0.002 3.76 3.19 3.93 3.63 3.63 3.93 3.76 2.36 3.93 0.001 3.76 0.001 3.76 PreserCyt 3.62 3.99 3.99 3.99 3.80 3.99 0.002 3.99 0.001 3.99 0.001 3.99 3.62 3.99 3.80 3.99 2.19 3.79 0.002 3.77 0.001 3.77 0.003 3.77 Batch 4 2SP 3.33 3.93 3.63 3.63 3.75 3.93 2.25 3.76 3.45 3.76 0.002 3.76 3.33 3.93 3.39 3.93 3.75 3.76 3.99 3.63 0.002 3.76 0.002 3.76 PreserCyt 3.62 3.99 3.79 3.79 3.99 3.99 0.003 3.99 0.002 3.79 0.002 3.79 3.49 3.99 3.99 3.79 3.79 3.79 0.003 3.99 0.002 3.79 0.003 3.79 ↵ a Results for duplicate assays are shown. Positive results are boldfaced..
- TABLE 2.
Performance of COBAS Amplicor CT/NG test for detection of C. trachomatis in ThinPrep Pap test samplesa
Age (yr) of female population (no. of persons)b No. of PCR results Specimens with inhibition Positive Negative 15 (n = 5) 1 4 None 16 (n = 13) 13 None 17 (n = 31) 1 30 None 18 (n = 67) 67 None 19 (n = 76) 76 None 20 (n = 130) 5 125 None 21 (n = 132) 4 128 None 22 (n = 117) 4 113 None 23 (n = 150) 2 148 None 24 (n = 124) 3 121 None 25 (n = 155) 2 153 None Total (n = 1,000) 22 978 None ↵ a None of the women tested showed clinical signs of urogenital infection, and cytological results were normal for all specimens.\
↵ b C. trachomatis PCR results were positive for 2 of the 116 15- to 18-year-olds tested (prevalence, 1.7%) and for 20 of the 884 19- to 25-year-olds tested (prevalence, 2.3%).
- TABLE 3.
Impact of storage conditions on the performance of the COBAS Amplicor CT/NG test
Sample no. C. trachomatis A660 at: Day 0 Day 15 Day 30 Day 45 1a 3.04 3.05 3.05 2.73 2a 3.08 2.95 3.01 2.77 3a 3.16 3.37 2.95 3.48 4 >4 >4 >4 2.76 5 2.97 2.95 2.47 1.95 6 1.97 3.13 3.37 3.10 7a 3.29 3.29 3.42 3.43 8a 3.03 3.33 2.26 2.98 9 3.37 3.72 3.73 3.73 10a 3.72 3.55 3.33 3.21 11 3.85 3.73 3.43 3.74 12 3.28 3.10 2.64 2.77 13 3.21 3.33 3.13 3.13 14a >4 3.26 NDb 3.56 15 3.18 3.11 3.64 3.16 16 2.78 3.86 ND 3.39 17a 3.08 3.64 3.11 3.19 18a 2.78 3.51 3.26 2.83 19 3.09 3.29 ND 1.94 20 3.44 3.03 ND 2.15 21 2.69 ND ND 2.69 22 3.06 ND ND 1.72