PreservCyt Transport Medium Used for the ThinPrep Pap Test Is a Suitable Medium for Detection of Chlamydia trachomatis by the COBAS Amplicor CT/NG Test: Results of a Preliminary Study and Future Implications

Anne Bianchi; François Moret; Jean-Marc Desrues; Thierry Champenois; Yves Dervaux; Orlane Desvouas; André Oursin; Dominique Quinzat; Roger Dachez; Christian Bathelier; Christophe Ronsin

doi:10.1128/JCM.40.5.1749-1754.2002

DOI: 10.1128/JCM.40.5.1749-1754.2002

Article Figures & Data

Tables

TABLE 1.

Comparison of the sensitivities of 2SP and PreservCyt (ThinPrep Pap test) for detection of C. trachomatis by the COBAS Amplicor CT/NG test

Batch and medium	A₆₆₀ of the following dilution (IFU/ml)^a:
	10³		10²		10		1		0.1		0.01
	C. trachomatis	IC	C. trachomatis	IC	C. trachomatis	IC	C. trachomatis	IC	C. trachomatis	IC	C. trachomatis	IC
Batch 1
2SP	3.24	3.84	3.54	3.99	3.99	3.99	3.99	3.99	2.03	3.99	0.003	3.99
	3.21	3.99	3.62	3.99	3.85	3.99	3.99	3.99	0.007	3.99	0.003	3.99
PreserCyt	3.17	3.84	3.72	3.99	3.99	3.99	3.42	3.99	0.001	3.99	0.001	3.99
	3.28	3.48	3.84	3.84	3.99	1.00	3.99	0.002	3.99	0.002	3.99
Batch 2
2SP	3.30	3.99	3.78	3.99	3.99	3.99	0.002	3.99	0.002	3.99	0.002	3.76
	3.23	3.99	3.60	3.77	3.99	3.77	3.12	3.77	0.002	3.77	0.002	3.77
PreserCyt	3.42	3.99	3.99	3.99	3.42	3.99	0.002	3.99	0.002	3.99	0.003	3.99
	3.62	3.99	3.84	3.99	3.99	3.99	0.003	3.99	0.002	3.99	0.002	3.93
Batch 3
2SP	3.19	3.99	3.63	3.99	3.63	3.93	3.93	3.93	1.99	3.76	0.002	3.76
	3.19	3.93	3.63	3.63	3.93	3.76	2.36	3.93	0.001	3.76	0.001	3.76
PreserCyt	3.62	3.99	3.99	3.99	3.80	3.99	0.002	3.99	0.001	3.99	0.001	3.99
	3.62	3.99	3.80	3.99	2.19	3.79	0.002	3.77	0.001	3.77	0.003	3.77
Batch 4
2SP	3.33	3.93	3.63	3.63	3.75	3.93	2.25	3.76	3.45	3.76	0.002	3.76
	3.33	3.93	3.39	3.93	3.75	3.76	3.99	3.63	0.002	3.76	0.002	3.76
PreserCyt	3.62	3.99	3.79	3.79	3.99	3.99	0.003	3.99	0.002	3.79	0.002	3.79
	3.49	3.99	3.99	3.79	3.79	3.79	0.003	3.99	0.002	3.79	0.003	3.79

↵ a Results for duplicate assays are shown. Positive results are boldfaced..

TABLE 2.

Performance of COBAS Amplicor CT/NG test for detection of C. trachomatis in ThinPrep Pap test samples^a

Age (yr) of female population (no. of persons)^b	No. of PCR results		Specimens with inhibition
Age (yr) of female population (no. of persons)^b	Positive	Negative	Specimens with inhibition
15 (n = 5)	1	4	None
16 (n = 13)		13	None
17 (n = 31)	1	30	None
18 (n = 67)		67	None
19 (n = 76)		76	None
20 (n = 130)	5	125	None
21 (n = 132)	4	128	None
22 (n = 117)	4	113	None
23 (n = 150)	2	148	None
24 (n = 124)	3	121	None
25 (n = 155)	2	153	None
Total (n = 1,000)	22	978	None

↵ a None of the women tested showed clinical signs of urogenital infection, and cytological results were normal for all specimens.\
↵ b C. trachomatis PCR results were positive for 2 of the 116 15- to 18-year-olds tested (prevalence, 1.7%) and for 20 of the 884 19- to 25-year-olds tested (prevalence, 2.3%).

TABLE 3.

Impact of storage conditions on the performance of the COBAS Amplicor CT/NG test

Sample no.	C. trachomatis A₆₆₀ at:
Sample no.	Day 0	Day 15	Day 30	Day 45
1^a	3.04	3.05	3.05	2.73
2^a	3.08	2.95	3.01	2.77
3^a	3.16	3.37	2.95	3.48
4	>4	>4	>4	2.76
5	2.97	2.95	2.47	1.95
6	1.97	3.13	3.37	3.10
7^a	3.29	3.29	3.42	3.43
8^a	3.03	3.33	2.26	2.98
9	3.37	3.72	3.73	3.73
10^a	3.72	3.55	3.33	3.21
11	3.85	3.73	3.43	3.74
12	3.28	3.10	2.64	2.77
13	3.21	3.33	3.13	3.13
14^a	>4	3.26	ND^b	3.56
15	3.18	3.11	3.64	3.16
16	2.78	3.86	ND	3.39
17^a	3.08	3.64	3.11	3.19
18^a	2.78	3.51	3.26	2.83
19	3.09	3.29	ND	1.94
20	3.44	3.03	ND	2.15
21	2.69	ND	ND	2.69
22	3.06	ND	ND	1.72