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Journal of Clinical Microbiology
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Bacteriology

Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs

D. Jonas, M. Speck, F. D. Daschner, H. Grundmann
D. Jonas
1National Reference Center of Hygiene, Institute of Environmental Medicine and Hospital Epidemiology, University Hospital Freiburg, 79106 Freiburg, Germany
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  • For correspondence: djonas@IUK3.UKL.uni-Freiburg.de
M. Speck
1National Reference Center of Hygiene, Institute of Environmental Medicine and Hospital Epidemiology, University Hospital Freiburg, 79106 Freiburg, Germany
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F. D. Daschner
1National Reference Center of Hygiene, Institute of Environmental Medicine and Hospital Epidemiology, University Hospital Freiburg, 79106 Freiburg, Germany
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H. Grundmann
2Department of Microbiology, University Hospital, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom
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DOI: 10.1128/JCM.40.5.1821-1823.2002
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ABSTRACT

A PCR identification of methicillin-resistant Staphylococcus aureus (MRSA), obviating the need for subculture on agar media, was investigated. The combination of MRSA detection by mecA femB PCR with prior enrichment in selective broth was tested for 439 swabs. PCR identified 36 MRSA-positive samples, in concordance with conventional methods.

  • Copyright © 2002 American Society for Microbiology
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Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs
D. Jonas, M. Speck, F. D. Daschner, H. Grundmann
Journal of Clinical Microbiology May 2002, 40 (5) 1821-1823; DOI: 10.1128/JCM.40.5.1821-1823.2002

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Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs
D. Jonas, M. Speck, F. D. Daschner, H. Grundmann
Journal of Clinical Microbiology May 2002, 40 (5) 1821-1823; DOI: 10.1128/JCM.40.5.1821-1823.2002
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    • ABSTRACT
    • Specimens.
    • Selective enrichment broth.
    • Duplex PCR.
    • Findings.
    • Conclusions.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
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KEYWORDS

Bacterial Proteins
Hexosyltransferases
methicillin resistance
Peptidyl Transferases
polymerase chain reaction
Staphylococcal Infections
Staphylococcus aureus

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