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Journal of Clinical Microbiology
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Clinical Veterinary Microbiology

Random Amplified Polymorphic DNA and Amplified Fragment Length Polymorphism Analyses of Pasteurella multocida Isolates from Fatal Fowl Cholera Infections

Brad S. Huber, Darin V. Allred, John C. Carmen, David D. Frame, David G. Whiting, Jason R. Cryan, Terry R. Olson, Paul J. Jackson, Karen Hill, Miriam T. Laker, Richard A. Robison
Brad S. Huber
1Department of Microbiology
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Darin V. Allred
1Department of Microbiology
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John C. Carmen
1Department of Microbiology
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David D. Frame
2Moroni Feed Company, Moroni, Utah 84646
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David G. Whiting
3Department of Statistics
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Jason R. Cryan
4Department of Zoology, Brigham Young University, Provo, Utah 84602
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Terry R. Olson
2Moroni Feed Company, Moroni, Utah 84646
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Paul J. Jackson
5Environmental and Molecular Biology, Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Karen Hill
5Environmental and Molecular Biology, Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Miriam T. Laker
5Environmental and Molecular Biology, Los Alamos National Laboratory, Los Alamos, New Mexico 87545
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Richard A. Robison
1Department of Microbiology
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  • For correspondence: richard_robison@byu.edu
DOI: 10.1128/JCM.40.6.2163-2168.2002
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    FIG. 1.

    Contour map of Sanpete County, Utah, showing the geographic distributions of isolates color coded by serotype. See Fig. 3 for serotype key.

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    FIG. 2.

    Portions of three electropherograms generated from AFLP analysis of three P. multocida isolates. The M-9 vaccine strain and isolate T29 are genetically similar. Isolate T25 is genetically dissimilar to the M-9 vaccine strain.

  • FIG. 3.
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    FIG. 3.

    Cluster analysis tree of the concatenated AFLP analysis data generated by the neighbor-joining method. Isolates from vaccinated turkeys are identified by a V prefix, while unvaccinated turkeys are identified by a T prefix. Isolates are color coded according to serotype (see key).

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Random Amplified Polymorphic DNA and Amplified Fragment Length Polymorphism Analyses of Pasteurella multocida Isolates from Fatal Fowl Cholera Infections
Brad S. Huber, Darin V. Allred, John C. Carmen, David D. Frame, David G. Whiting, Jason R. Cryan, Terry R. Olson, Paul J. Jackson, Karen Hill, Miriam T. Laker, Richard A. Robison
Journal of Clinical Microbiology Jun 2002, 40 (6) 2163-2168; DOI: 10.1128/JCM.40.6.2163-2168.2002

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Random Amplified Polymorphic DNA and Amplified Fragment Length Polymorphism Analyses of Pasteurella multocida Isolates from Fatal Fowl Cholera Infections
Brad S. Huber, Darin V. Allred, John C. Carmen, David D. Frame, David G. Whiting, Jason R. Cryan, Terry R. Olson, Paul J. Jackson, Karen Hill, Miriam T. Laker, Richard A. Robison
Journal of Clinical Microbiology Jun 2002, 40 (6) 2163-2168; DOI: 10.1128/JCM.40.6.2163-2168.2002
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KEYWORDS

Pasteurella Infections
Pasteurella multocida
Polymorphism, Restriction Fragment Length
Poultry Diseases
Random Amplified Polymorphic DNA Technique

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