Article Figures & Data
Figures
- FIG. 1.
Relationship between the CT and log10 CMV copy number, showing linearity. The CMV copy number represents copies of plasmid pJSCPOL.
- FIG. 2.
Serial dilutions of a CMV-positive patient specimen, demonstrating the linearity of both the CMV and human DNA PCR assays. The calculated CMV viral load (CMV copies per microgram of human DNA) remained stable despite dilution of the original specimen.
- FIG. 3.
Effects of interval from blood draw to plasma separation and storage temperature on levels of human DNA in plasma. Panels A and B show data for two different volunteers.
- FIG. 4.
Effect of plasma separation method on the amount of human DNA in plasma. The human DNA copy number was measured in specimens from four different volunteers after each specimen was processed according to each of four different procedures.
Tables
- TABLE 1.
Effect of human DNA prepared from whole blood or leukocyte lysate on detection of CMV DNA
No. of CMV plasmid copies Mean CT in water Mean CT (ΔCT)a in: Whole-blood DNA Leukocyte lysate 106 26.12 27.27 (1.15)b 30.59 (4.47)b 103 33.79 36.08 (2.29)b 40.08 (6.29)b 102 36.97 39.75 (2.78)b 42.90 (5.93)b ↵ a Mean CT values are from samples run in triplicate. The CT is the PCR cycle at which the CMV detection threshold was exceeded. ΔCT is the difference between the mean CT of samples in the indicated backgrounds and the mean CT of samples in water.
↵ b The difference from the mean CT in water is significant (P < 0.01 by an unpaired t test). The mean differences between the CT in whole-blood DNA and the CT in leukocyte lysate are also statistically significant (P < 0.01 by an unpaired t test).
