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Clinical Veterinary Microbiology

Differentiation of Foot-and-Mouth Disease Virus-Infected from Vaccinated Pigs by Enzyme-Linked Immunosorbent Assay Using Nonstructural Protein 3AB as the Antigen and Application to an Eradication Program

Wen-Bin Chung, Karl Johan Sorensen, Pei-Chih Liao, Pin-Cheng Yang, Ming-Hwa Jong
Wen-Bin Chung
1Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung
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  • For correspondence: wbchung@mail.npust.edu.tw
Karl Johan Sorensen
2Danish Veterinary Institute for Virus Research, Kalvehave, Denmark
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Pei-Chih Liao
1Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung
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Pin-Cheng Yang
3Department of Comparative Medicine, Pig Research Institute, Taiwan, Miaoli
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Ming-Hwa Jong
4National Institute for Animal Health, Council of Agriculture, Tansui, Taiwan, Republic of China
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DOI: 10.1128/JCM.40.8.2843-2848.2002
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  • FIG. 1.
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    FIG. 1.

    Percent frequency distribution of ODp values of sera from naïve, vaccinated, and infected pigs. (a) Sera from 100 nonvaccinated and noninfected pigs; (b) sera from 120 infected pigs; (c) sera from 106 vaccinated pigs. Pigs with ODp values of ≤75 are test positive (+) for antibody against FMDV 3AB antigen, and pigs with ODp values of >75 are test negative (−).

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    FIG. 2.

    Percent frequency distribution of pigs with positive results in ELISA following natural exposure to FMDV. Vesicular lesions were observed first in 7-week-old nursery pigs on one pig farm 5 days before the first bleeding (designated week 1 postoutbreak). Eight to 10 sera were collected from pigs in the same pen with the first case. Shaded bars, percentage of pigs with ODp values of ≤75 (test positive); solid circles, means ± SD of ODp values.

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    FIG. 3.

    Percent frequency distribution of ODp values of sera from sows at parities 9 to 12 with or without prior FMDV infection. (a) Sera collected from pig farms without outbreak of FMD. Each sow had received at least 10 vaccinations; n = 20. (b) Sera collected from pig farms 3.5 years after outbreak of FMD. Each sow had received at least six vaccinations; n = 20. Pigs with ODp values of ≤75 are test positive (+), and pigs with ODp values of >75 are test negative (−).

  • FIG. 4.
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    FIG. 4.

    Persistence of maternally derived antibodies against FMDV 3AB antigen in piglets born from naturally infected sows. Sequential sera were collected from three piglets of each of 3 sows. Shaded bars, percentage of piglets with ODp values of ≤75 (test positive). Solid circle, mean ± SD of ODp values.

  • FIG. 5.
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    FIG. 5.

    Percentages of sows and finishers with positive results in ELISA at various intervals after the outbreak of FMD in Taiwan. A total of 10, 15, 179, and 174 pig farms were surveyed for sows 2 (solid bars), 2.5 (shaded bars), 3 (hatched bars), and 3.5 (open bars) years postoutbreak, respectively. Ten sera each from sows at parities 1 to 2, 3 to 4, and ≧5 were randomly collected from each pig farm. A total of 9, 15, 199, and 188 farms were surveyed for finishers 2, 2.5, 3, and 3.5 years postoutbreak, respectively. Thirty finishers were randomly selected from each pig farm for bleeding, except for 2.5 years postoutbreak, when only 10 pigs per farm were tested.

Tables

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  • TABLE 1.

    Correlation coefficients between the paired sow serum and colostrum and offspring serum test results for antibodies to the FMDV 3AB antigen

    FarmaNo. of sows testedCorrelation coefficient between:
    Sow sera and colostrumSow sera and offspring seraColostrum and offspring sera
    CL250.360.890.48
    SW171.00.890.89
    HS430.950.950.90
    Total850.860.930.81
    • ↵ a Sow sera, colostrums, and offspring sera were collected from three pig farms 2 years after the outbreak of FMD. At least half of the sows selected had been naturally exposed to FMDV for each pig farm.

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Differentiation of Foot-and-Mouth Disease Virus-Infected from Vaccinated Pigs by Enzyme-Linked Immunosorbent Assay Using Nonstructural Protein 3AB as the Antigen and Application to an Eradication Program
Wen-Bin Chung, Karl Johan Sorensen, Pei-Chih Liao, Pin-Cheng Yang, Ming-Hwa Jong
Journal of Clinical Microbiology Aug 2002, 40 (8) 2843-2848; DOI: 10.1128/JCM.40.8.2843-2848.2002

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Differentiation of Foot-and-Mouth Disease Virus-Infected from Vaccinated Pigs by Enzyme-Linked Immunosorbent Assay Using Nonstructural Protein 3AB as the Antigen and Application to an Eradication Program
Wen-Bin Chung, Karl Johan Sorensen, Pei-Chih Liao, Pin-Cheng Yang, Ming-Hwa Jong
Journal of Clinical Microbiology Aug 2002, 40 (8) 2843-2848; DOI: 10.1128/JCM.40.8.2843-2848.2002
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KEYWORDS

Antibodies, Viral
Foot-and-Mouth Disease
foot-and-mouth disease virus
Swine Diseases
Viral Nonstructural Proteins
Viral Vaccines

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