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Bacteriology

Evaluation of an Enzyme Immunoassay Technique for Detection of Antibodies against Treponema pallidum

Rita Castro, Emília S. Prieto, Irene Santo, Jacinta Azevedo, Filomena da L. Exposto
Rita Castro
1Unidade de Doenças Sexualmente Transmitidas, Centro de Malária e outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical
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  • For correspondence: ritacastro@ihmt.unl.pt
Emília S. Prieto
1Unidade de Doenças Sexualmente Transmitidas, Centro de Malária e outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical
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Irene Santo
2Centro de Saúde da Lapa, Ministério da Saúde, Lisbon, Portugal
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Jacinta Azevedo
2Centro de Saúde da Lapa, Ministério da Saúde, Lisbon, Portugal
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Filomena da L. Exposto
1Unidade de Doenças Sexualmente Transmitidas, Centro de Malária e outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical
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DOI: 10.1128/JCM.41.1.250-253.2003
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Tables

  • TABLE 1.

    Qualitative results obtained by the ELISA and MHA-TPa

    IgG ELISA resultNo. of samples with the following MHA-TP result:
    ReactiveNonreactiveTotal
    Reactive3139322
    Nonreactive0119119
    Total313128441
    • ↵ a Sensitivity (313 of 313 samples), 100%; specificity (119 of 128 samples), 93%; agreement (432 of 441 samples), 98%.

  • TABLE 2.

    Qualitative results obtained by the ELISA and FTA-Abs testa

    IgG ELISA resultNo. of samples with the following FTA-ABS test result:
    ReactiveNonreactiveTotal
    Reactive3220322
    Nonreactive2117119
    Total324117441
    • ↵ a Sensitivity (322 of 324 samples), 99.4%; specificity (117 of 117 samples), 100%; agreement (439 of 441 samples), 99.5%.

  • TABLE 3.

    Sensitivities and specificities of RPR test, MHA-TP, and IgG ELISA in comparison to results of FTA-Abs test for IgG

    Syphilis stageaRPR testMHA-TPIgG ELISA
    SensitivitybSpecificitycSensitivitySpecificitySensitivitySpecificity
    PS23/25 (92)22/25 (88)25/25 (100)
    SS25/25 (100)25/25 (100)25/25 (100)
    LS174/179 (97.2)178/179 (99.4)179/179 (100)
    PTS55/95 (57.9)88/95 (92.6)93/95 (97.9)
    NHS95/107 (88.8)107/107 (100)107/107 (100)
    • ↵ a PS, primary syphilis; SS, secondary syphilis; LS, latent syphilis; PTS, past treated syphilis; NHS, no history of syphilis.

    • ↵ b The data represent the number of samples positive by the test/number of samples positive by the FTA-Abs test (percent).

    • ↵ c The data represent the number of samples negative by the test/number of samples negative by the FTA-Abs test (percent).

  • TABLE 4.

    Detection of anti-T. pallidum IgM antibodies during early syphilis by the FTA-Abs test for IgM and the ELISA for IgM

    Syphilis stageaFTA-Abs test for IgMELISA for IgM
    No. of reactive samples/total no. of samples tested (%)No. of nonreactive samples/total no. of samples tested (%)No. of samples with indeterminate results/total no. of samples tested (%)No. of reactive samples/total no. of samples tested (%)No. of nonreactive samples/total no. of samples tested (%)No. of samples with indeterminate results/total no. of samples tested (%)
    PS18/25 (72)1/25 (4)6/25 (24)22/25 (88)2/25 (8)1/25 (4)
    SS17/25 (68)6/25 (24)2/25 (8)19/25 (76)5/25 (20)1/25 (4)
    LS30/179 (17)143/179 (80)6/179 (3.3)34/179 (19)143/179 (79.9)2/179 (1.1)
    Total65/229 (28.4)150/229 (65.5)14/229 (6.1)75/229 (32.8)150/229 (65.5)4/229 (1.7)
    • ↵ a PS, primary syphilis; SS, secondary syphilis; LS, latent syphilis.

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Evaluation of an Enzyme Immunoassay Technique for Detection of Antibodies against Treponema pallidum
Rita Castro, Emília S. Prieto, Irene Santo, Jacinta Azevedo, Filomena da L. Exposto
Journal of Clinical Microbiology Jan 2003, 41 (1) 250-253; DOI: 10.1128/JCM.41.1.250-253.2003

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Evaluation of an Enzyme Immunoassay Technique for Detection of Antibodies against Treponema pallidum
Rita Castro, Emília S. Prieto, Irene Santo, Jacinta Azevedo, Filomena da L. Exposto
Journal of Clinical Microbiology Jan 2003, 41 (1) 250-253; DOI: 10.1128/JCM.41.1.250-253.2003
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KEYWORDS

Antibodies, Bacterial
Immunoenzyme Techniques
syphilis
Treponema pallidum

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