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Bacteriology

Association between Handling of Pet Treats and Infection with Salmonella enterica Serotype Newport Expressing the AmpC β-Lactamase, CMY-2

Johann D. D. Pitout, Mark D. Reisbig, Mike Mulvey, Linda Chui, Marie Louie, Larry Crowe, Deirdre L. Church, Sameer Elsayed, Dan Gregson, Rafiq Ahmed, Peter Tilley, Nancy D. Hanson
Johann D. D. Pitout
1Division of Microbiology, Calgary Laboratory Services and Department of Pathology & Laboratory Medicine, University of Calgary
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  • For correspondence: johann.pitout@cls.ab.ca
Mark D. Reisbig
2Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska
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Mike Mulvey
3National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, Health Canada, Winnipeg, Manitoba, Canada
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Linda Chui
4Provincial Laboratory for Public Health (Microbiology) and Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta
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Marie Louie
4Provincial Laboratory for Public Health (Microbiology) and Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta
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Larry Crowe
5Food Borne Illness Investigations, Environmental Health, Calgary Health Region
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Deirdre L. Church
1Division of Microbiology, Calgary Laboratory Services and Department of Pathology & Laboratory Medicine, University of Calgary
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Sameer Elsayed
1Division of Microbiology, Calgary Laboratory Services and Department of Pathology & Laboratory Medicine, University of Calgary
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Dan Gregson
1Division of Microbiology, Calgary Laboratory Services and Department of Pathology & Laboratory Medicine, University of Calgary
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Rafiq Ahmed
3National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, Health Canada, Winnipeg, Manitoba, Canada
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Peter Tilley
6Provincial Laboratory for Public Health, Calgary, Alberta
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Nancy D. Hanson
2Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska
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DOI: 10.1128/JCM.41.10.4578-4582.2003
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    FIG. 1.

    PFGE analysis of DNA isolated from human Salmonella strains (strains Snp1, Snp5, Snp6, and Snp7) and a Salmonella strain from a commercial product (strain Snp8). DNA was cleaved with the endonuclease XbaI or BlnI. Snp1, Snp5, Snp6, Snp7, and Snp8 designate the strains described in the text. Lanes M, S. enterica serotype Newport AM01144 (915 to 35.8 kb), used as a size marker. The braces indicate the restriction endonuclease used for each digestion.

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  • TABLE 1.

    S. enterica serotype Newport strains isolated from humans in Alberta, Canada, from January 1998 to December 2002

    YrNo. of Salmonella sp.aNo. of Salmonella serotype Newport isolatesPTsbNo. of strains resistant to:
    AMPCAZFOXCHLSXT
    19981,13898, 9, 1400000
    19991,106168, 9, 13, 1400000
    20001,0658NT, 1400000
    20011,24473, 4, 8, 9, 1000000
    2002889214, 8, 9, 10, 14, 15, 1799991
    • ↵ a Total number of Salmonella species isolated from humans in Alberta, Canada.

    • ↵ b Resistant strains were PT 14 (eight of nine) and PT 17 (one of nine). NT, nontypeable.

  • TABLE 2.

    MICs for clinical strains of S. enterica serotype Newport

    Clinical strainaOriginMIC (μg/ml)
    AMPSAMFOXCPDCAZCTX and CRObATMFEPCHLSXTGENTOBAMKCIP
    Snp1Patient A>16>16/8>16>8>161616≤2>16≤1/19≤0.25≤0.25≤1≤0.5
    Snp2Patient A>16>16/8>16>8>16>32>168>16≤1/19≤0.25≤0.25≤1≤0.5
    Snp3Patient A>16>16/8>16>8>16>32>168>16≤1/19≤0.25≤0.25≤1≤0.5
    Snp4Patient B>16>16/8>16>8>161616≤2>16≤1/19≤0.25≤0.25≤1≤0.5
    Snp5Patient C>16>16/8>16>8>161616≤2>16≤1/19≤0.25≤0.25≤1≤0.5
    Snp6Patient D>16>16/8>16>8>161616≤2>16≤1/19≤0.25≤0.25≤1≤0.5
    Snp7Patient E>16>16/8>16>8>161616≤2>16≤1/19≤0.25≤0.25≤1≤0.5
    Snp8Pet treat>16>16/8>16>8>161616≤2>16≤1/19≤0.25≤0.25≤1≤0.5
    • ↵ a Snp1 to Snp8 designate the strains described in the text.

    • ↵ b The MICs of CRO and CTX were identical.

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Association between Handling of Pet Treats and Infection with Salmonella enterica Serotype Newport Expressing the AmpC β-Lactamase, CMY-2
Johann D. D. Pitout, Mark D. Reisbig, Mike Mulvey, Linda Chui, Marie Louie, Larry Crowe, Deirdre L. Church, Sameer Elsayed, Dan Gregson, Rafiq Ahmed, Peter Tilley, Nancy D. Hanson
Journal of Clinical Microbiology Oct 2003, 41 (10) 4578-4582; DOI: 10.1128/JCM.41.10.4578-4582.2003

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Association between Handling of Pet Treats and Infection with Salmonella enterica Serotype Newport Expressing the AmpC β-Lactamase, CMY-2
Johann D. D. Pitout, Mark D. Reisbig, Mike Mulvey, Linda Chui, Marie Louie, Larry Crowe, Deirdre L. Church, Sameer Elsayed, Dan Gregson, Rafiq Ahmed, Peter Tilley, Nancy D. Hanson
Journal of Clinical Microbiology Oct 2003, 41 (10) 4578-4582; DOI: 10.1128/JCM.41.10.4578-4582.2003
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KEYWORDS

Animal Feed
Animals, Domestic
Salmonella Infections
Salmonella enterica
beta-lactamases

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