Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Clinical Microbiology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Bacteriology

Evolution of a Vancomycin-Intermediate Staphylococcus aureus Strain In Vivo: Multiple Changes in the Antibiotic Resistance Phenotypes of a Single Lineage of Methicillin-Resistant S. aureus under the Impact of Antibiotics Administered for Chemotherapy

K. Sieradzki, T. Leski, J. Dick, L. Borio, A. Tomasz
K. Sieradzki
1The Rockefeller University, New York, New York 10021
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
T. Leski
1The Rockefeller University, New York, New York 10021
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. Dick
2John Hopkins Medical Institutions, Baltimore, Maryland 21287
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
L. Borio
2John Hopkins Medical Institutions, Baltimore, Maryland 21287
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A. Tomasz
1The Rockefeller University, New York, New York 10021
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: tomasz@mail.rockefeller.edu
DOI: 10.1128/JCM.41.4.1687-1693.2003
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    Schematic representation of antibiotic exposure and recovery of MRSA isolates as a function of calendar dates (month/day/year). Antibiotic concentrations are given in micrograms per milliliter.

  • FIG. 2.
    • Open in new tab
    • Download powerpoint
    FIG. 2.

    PFGE patterns of JH isolates recovered at different times during the patient's clinical history. Chromosomal DNA was prepared, SmaI restricted, and separated by PFGE. For identification of the isolates, see Table 1.

  • FIG. 3.
    • Open in new tab
    • Download powerpoint
    FIG. 3.

    Oxacillin (top panel) and vancomycin (bottom panel) susceptibility profiles for JH isolates. Bacterial cultures were grown and plated on agar containing various concentrations of antibiotics for population analysis.

  • FIG. 4.
    • Open in new tab
    • Download powerpoint
    FIG. 4.

    Enrichment of the JH1 culture for a subpopulation of bacteria with elevated oxacillin resistance. Isolate JH1 was plated for population analysis on agar containing increasing concentrations of imipenem. Bacterial cells capable of forming colonies on agar containing 50 μg of imipenen/ml (arrow) were picked, diluted in drug-free TSB, grown overnight, and plated for population analysis on both imipenem- and oxacillin-containing plates.

  • FIG. 5.
    • Open in new tab
    • Download powerpoint
    FIG. 5.

    Enrichment of the JH1 culture for subpopulations of bacteria for which vancomycin MICs were elevated. Isolate JH1 was plated for population analysis on agar containing increasing concentrations of vancomycin. Bacterial cells capable of forming colonies on agar containing 4.0 μg of vancomycin/ml (arrow) were picked, diluted in drug-free TSB, grown overnight, and plated for population analysis (mutant JH1P4). Extended exposure of mutant JH1P4 to gradually increasing concentrations of vancomycin in growth medium eventually allowed for the selection of mutant JH1P4T4, for which the vancomycin MIC was 8.0 μg/ml.

  • FIG. 6.
    • Open in new tab
    • Download powerpoint
    FIG. 6.

    Suppression of phenotypic expression of resistance to oxacillin by selection of vancomycin resistance in isolate JH3. Bacterial cultures were grown in TSB to stationary phase and plated at several dilutions on TSA containing various concentrations of vancomycin. Single colonies that could grow at concentrations of vancomycin above the MICs for the majority of the cells were picked and suspended in growth medium supplemented with the same drug concentrations. After several cycles of selection, a mutant (JH3V8) for which the vancomycin MIC was 16 μg/ml was obtained.

  • FIG. 7.
    • Open in new tab
    • Download powerpoint
    FIG. 7.

    Changes in susceptibility to the bactericidal effect of vancomycin. The viability of isolates JH 1 and JH14 was determined during exposure to vancomycin at concentrations corresponding to 1, 2, 5, and 10 times the MIC. Exponentially growing cultures were exposed to the antibiotic (arrow), and aliquots were removed at various times to determine the viable titer of bacteria as described in Materials and Methods.

  • FIG. 8.
    • Open in new tab
    • Download powerpoint
    FIG. 8.

    Vancomycin susceptibility of strain PA237. The strain was grown overnight in TSB and then plated for population analysis on TSA plates containing various concentrations of vancomycin (○). A colony of strain PA237 growing on an agar plate containing 4 μg of vancomycin/ml was picked (arrow) and used as an inoculum for drug-free TSB to generate a new culture (PA237P4), the PAP for which is also shown (•).

Tables

  • Figures
  • TABLE 1.

    Genetic profiles of analyzed MRSA isolates

    IsolateDate obtained (mo/day)SourceMIC, μg/ml, of:MLSTspaA typePFGE pattern
    Oxacillin (hetero classa)Vancomycinb
    JH17/20Blood0.75 (1)1.01-4-1-4-12-1-28TJMBMDMGMKA0
    JH29/20Blood25 (3)4.01-4-1-4-12-1-28TJMBMDMGMKA0
    JH39/24Blood100 (3)4.01-4-1-4-12-1-28TJMBMDMGMKA0
    JH510/01Blood0.75 (1)6.01-4-1-4-12-1-28TJMBMDMGMKA0
    JH610/06Blood1.5 (1)8.01-4-1-4-12-1-28TJMBMDMGMKA0
    JH910/13Blood0.75 (1)8.01-4-1-4-12-1-28TJMBMDMGMKA0
    JH1410/17Heart valve1.5 (1)8.01-4-1-4-12-1-28TJMBMDMGMKA0
    JH1510/23Nares of contact0.75 (1)1.01-4-1-4-12-1-28TJMBMDMGMKA0
    PA2371.01-4-1-4-12-1-10TJMBMDMGMKA1
    PC38.01-4-1-4-12-1-10TJMBMDMGMKA1
    Mu508.01-4-1-4-12-1-10TJMBMDMGMKA2
    M18.01-4-1-4-12-1-10TJMGMKA1
    NJ8.01-4-1-4-12-1-10TMBMDMGMKA3
    • ↵ a As defined in reference 18.

    • ↵ b As determined by the E-Test.

PreviousNext
Back to top
Download PDF
Citation Tools
Evolution of a Vancomycin-Intermediate Staphylococcus aureus Strain In Vivo: Multiple Changes in the Antibiotic Resistance Phenotypes of a Single Lineage of Methicillin-Resistant S. aureus under the Impact of Antibiotics Administered for Chemotherapy
K. Sieradzki, T. Leski, J. Dick, L. Borio, A. Tomasz
Journal of Clinical Microbiology Apr 2003, 41 (4) 1687-1693; DOI: 10.1128/JCM.41.4.1687-1693.2003

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Journal of Clinical Microbiology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Evolution of a Vancomycin-Intermediate Staphylococcus aureus Strain In Vivo: Multiple Changes in the Antibiotic Resistance Phenotypes of a Single Lineage of Methicillin-Resistant S. aureus under the Impact of Antibiotics Administered for Chemotherapy
(Your Name) has forwarded a page to you from Journal of Clinical Microbiology
(Your Name) thought you would be interested in this article in Journal of Clinical Microbiology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Evolution of a Vancomycin-Intermediate Staphylococcus aureus Strain In Vivo: Multiple Changes in the Antibiotic Resistance Phenotypes of a Single Lineage of Methicillin-Resistant S. aureus under the Impact of Antibiotics Administered for Chemotherapy
K. Sieradzki, T. Leski, J. Dick, L. Borio, A. Tomasz
Journal of Clinical Microbiology Apr 2003, 41 (4) 1687-1693; DOI: 10.1128/JCM.41.4.1687-1693.2003
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • Antibiotic susceptibility.
    • Time-kill curves.
    • DNA manipulations.
    • MLST and spaA typing.
    • Detection of mecA.
    • RESULTS AND DISCUSSION
    • Changes in oxacillin and vancomycin susceptibilities as a function of the chronological date of isolation of JH strains.
    • Changes in growth rate and susceptibility to the bactericidal effect of vancomycin as a function of the chronological dates of isolation of JH strains.
    • Vancomycin susceptibility of MRSA strain PA237.
    • ADDENDUM
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

bacteremia
Drug Resistance, Bacterial
Staphylococcal Infections
Staphylococcus aureus
vancomycin

Related Articles

Cited By...

About

  • About JCM
  • Editor in Chief
  • Board of Editors
  • Editor Conflicts of Interest
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Resources for Clinical Microbiologists
  • Ethics
  • Contact Us

Follow #JClinMicro

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

 

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0095-1137; Online ISSN: 1098-660X