Differentiation between Isolates of Aspergillus fumigatus from Breeding Turkeys and Their Environment by Genotyping with Microsatellite Markers

DOI: 10.1128/JCM.41.4.1798-1800.2003

Article Figures & Data

TABLE 1.

Genotypes of A. fumigatus isolates collected from breeding turkeys

Age of bird, days (animal identification)	No. of isolates	No. of genotypes	Microsatellite genotype reference(s)^a
1-day-old chicks
1 (I)	4	1	A108B106C173D108
1 (II)	4	1	A108B106C173D108
1 (III)	4	1	A108B106C173D108
1 (IV)	4	1	A108B106C173D108
1 (V)	3	1	A108B106C173D108
1 (VI)	4	1	A108B106C173D108
Healthy turkeys (>1 day old)
25 (VII)	9	6	C171D110, C173D76, C175D76, C177D104, C181D82, C181D84
25 (VIII)	2	2	C175D76, C179D86
37 (IX)	5	2	C173D108, C173D110
44 (X)	4	3	C173D108, C175D76, C179D84
65 (XI)	14	2	C167D98, C173D118
65 (XII)	2	1	C173D76
65 (XIII)	16	3	C169D102, C173D76, C177D120
79 (XIV)	2	2	C171D98, C173D116
87 (XV)	1	1	C169D102
Turkeys with aspergillosis
106 (XVI)	27	1	C173D112
106 (XVII)	9	1	C169D102

↵ a Genotype reference comprises microsatellite loci (A, B, C, and D) and corresponding molecular sizes (in base pairs) of PCR products.

TABLE 2.

Genotypes of A. fumigatus isolates collected from air samples^a

Sampling week	No. of A. fumigatus isolates	No. of microsatellite genotypes	Microsatellite genotype reference(s)^b
3	3	3	C167D102, C171D110, C175D76
4	1	1	C171D110
5	7	5	C171D108, C171D124, C173D76, C173D110, C175D76, C181D106
6	30	18	C167D76, C167D84, C167D92, C167D96, C167D102, C167D104, C171D102, C171D110, C173D66, C173D76, C173D78, C173D98, C173D108, C173D114, C175D66, C175D76, C177D100, C183D78
7	15	8	C167D96, C167D104, C171D128, C173D86, C173D108, C175D76, C177D140, C185D98
8	3	3	C167D104, C173D82, C173D108
9	19	16	C167D84, C167D98, C167D100, C167D102, C167D104, C169D108, C171D64, C171D104, C171D110, C173D108, C171D122, C175D76, C177D60, C177D84, C179D86, C181D94
10	7	4	C167D100, C173D76, C173D108, C175D76
11	2	2	C173D116, C177D118
12	7	5	C167D84, C167D86, C173D114, C177D106, C181D126
13	4	4	C167D92, C169D102, C173D108, C179D114
14	22	9	C167D96, C167D98, C169D102, C169D112, C171D108, C171D110, C175D76, C177D110, C179D84
15	9	5	C171D108, C173D108, C175D76, C179D60, C179D152
16	5	5	C171D106, C171D110, C171D126, C177D82, C179D78

↵ a Environmental genotypes also detected in animals are indicated in bold. Genotypes concomitantly detected in air samples and in animals (the same week) are underlined
↵ b Genotype reference comprises microsatellite loci (C and D) and corresponding molecular sizes (in base pairs) of PCR products.