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Virology

In Vitro Detection of Dissimilar Amounts of Hepatitis C Virus (HCV) Subtype-Specific RNA Genomes in Mixes Prepared from Sera of Persons Infected with a Single HCV Genotype

Jorge F. Quarleri, María V. Bussy, Verónica L. Mathet, Vanesa Ruiz, Rubén Iácono, Ling Lu, Betty H. Robertson, José R. Oubiña
Jorge F. Quarleri
1Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
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María V. Bussy
1Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
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Verónica L. Mathet
1Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
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Vanesa Ruiz
1Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
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Rubén Iácono
1Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
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Ling Lu
2Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia
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Betty H. Robertson
2Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia
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José R. Oubiña
1Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
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  • For correspondence: joubina@fmed.uba.ar
DOI: 10.1128/JCM.41.6.2727-2733.2003
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Article Information

DOI 
https://doi.org/10.1128/JCM.41.6.2727-2733.2003
PubMed 
12791916

Published By 
American Society for Microbiology Journals
History 
  • Received November 15, 2002
  • Returned for modification January 8, 2003
  • Accepted January 25, 2003
  • Published online June 1, 2003.

Copyright & Usage 
Copyright © 2003 American Society for Microbiology

Author Information

  1. Jorge F. Quarleri1,
  2. María V. Bussy1,
  3. Verónica L. Mathet1,
  4. Vanesa Ruiz1,
  5. Rubén Iácono1,
  6. Ling Lu2,
  7. Betty H. Robertson2 and
  8. José R. Oubiña1*
  1. 1Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
  2. 2Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia
  • ↵* Corresponding author. Mailing address: Hepatitis Laboratory, Microbiology Department, School of Medicine, University of Buenos Aires, Paraguay 2155 - Piso 11, (1121) Buenos Aires, Argentina. Phone: (5411) 5950-9500/99. Fax: (5411) 4508-3705. E-mail address: joubina{at}fmed.uba.ar .
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In Vitro Detection of Dissimilar Amounts of Hepatitis C Virus (HCV) Subtype-Specific RNA Genomes in Mixes Prepared from Sera of Persons Infected with a Single HCV Genotype
Jorge F. Quarleri, María V. Bussy, Verónica L. Mathet, Vanesa Ruiz, Rubén Iácono, Ling Lu, Betty H. Robertson, José R. Oubiña
Journal of Clinical Microbiology Jun 2003, 41 (6) 2727-2733; DOI: 10.1128/JCM.41.6.2727-2733.2003

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In Vitro Detection of Dissimilar Amounts of Hepatitis C Virus (HCV) Subtype-Specific RNA Genomes in Mixes Prepared from Sera of Persons Infected with a Single HCV Genotype
Jorge F. Quarleri, María V. Bussy, Verónica L. Mathet, Vanesa Ruiz, Rubén Iácono, Ling Lu, Betty H. Robertson, José R. Oubiña
Journal of Clinical Microbiology Jun 2003, 41 (6) 2727-2733; DOI: 10.1128/JCM.41.6.2727-2733.2003
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  • Top
  • Article
    • ABSTRACT
    • Serum samples.
    • RT-nested PCR for detection of HCV RNAs in serum samples.
    • Viremia levels in samples used for in vitro RNA mixing assays.
    • HCV genotyping.
    • Mixing studies.
    • Computerized RNA secondary structure analysis.
    • Statistical analysis.
    • HCV isolate characterization.
    • Level of in vitro detection of subtype-specific HCV RNAs in prepared mixtures.
    • RFLP detects nonequimolar concentrations of artificially mixed subtype-specific HCV RNAs in vitro: epidemiological, clinical, and virological impact.
    • Nucleotide sequence accession numbers.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
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KEYWORDS

Genome, Viral
Hepacivirus
Hepatitis C, Chronic
Polymorphism, Restriction Fragment Length
RNA, Viral

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