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Epidemiology

Molecular Typing of Methicillin-Resistant Staphylococcus aureus: Can PCR Replace Pulsed-Field Gel Electrophoresis?

A. Strandén, R. Frei, A. F. Widmer
A. Strandén
1Division of Hospital Epidemiology
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R. Frei
2Bacteriology Laboratory, University Hospital Basel, CH-3031 Basel, Switzerland
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A. F. Widmer
1Division of Hospital Epidemiology
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  • For correspondence: awidmer@uhbs.ch
DOI: 10.1128/JCM.41.7.3181-3186.2003
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  • FIG. 1.
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    FIG. 1.

    Dendrogram of SmaI-PFGE of epidemiologically unrelated strains. Strain numbers are indicated in column A; PFGE types are indicated in column B.

  • FIG. 2.
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    FIG. 2.

    Dendrogram of PCR of epidemiologically unrelated strains. Strain numbers are indicated in column A; PCR types are indicated in column B.

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    FIG. 3.

    Dendrogram of SmaI-PFGE (A) and PCR results (B) from epidemic strains. Strain numbers are indicated on the right side. Strain 84 is the unrelated control.

  • FIG. 4.
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    FIG. 4.

    Agarose gel electrophoresis of PCR-amplified HVR adjacent to the mecA, spa, and coa genes. Strain numbers are indicated on the top of the lanes. Lane M, size marker. Molecular sizes are indicated at the left in base pairs.

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    FIG. 5.

    Agarose gel electrophoresis of PCR products of clinical unrelated isolates (A) and epidemic strains (B), with strain 84 as a positive amplification control. K, negative control. Strain numbers are indicated at the top of the lanes. Lane M, molecular size marker.

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  • TABLE 1.

    Comparison of PCR versus PFGE typing resultsa

    PCR round and resultNo of strains tested by PFGE
    PositiveNegative
    First
        Positive329
        Negative1222
    Second
        Positive3511
        Negative621
    • ↵ a Strains typed by both PFGE and PCR (+/+ strains) were clustered together by PFGE and PCR. Strains typed by neither method (−/− strains) were unrelated by PFGE and PCR. The +/− or −/+ strains were clustered together by PFGE but not by PCR or by PCR but not by PFGE as indicated. In the PCR second run, two strains were not typeable.

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Molecular Typing of Methicillin-Resistant Staphylococcus aureus: Can PCR Replace Pulsed-Field Gel Electrophoresis?
A. Strandén, R. Frei, A. F. Widmer
Journal of Clinical Microbiology Jul 2003, 41 (7) 3181-3186; DOI: 10.1128/JCM.41.7.3181-3186.2003

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Molecular Typing of Methicillin-Resistant Staphylococcus aureus: Can PCR Replace Pulsed-Field Gel Electrophoresis?
A. Strandén, R. Frei, A. F. Widmer
Journal of Clinical Microbiology Jul 2003, 41 (7) 3181-3186; DOI: 10.1128/JCM.41.7.3181-3186.2003
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    • ABSTRACT
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KEYWORDS

Electrophoresis, Gel, Pulsed-Field
methicillin resistance
polymerase chain reaction
Staphylococcus aureus

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