Article Figures & Data
Figures
- FIG. 1.
Dendrogram of SmaI-PFGE of epidemiologically unrelated strains. Strain numbers are indicated in column A; PFGE types are indicated in column B.
- FIG. 2.
Dendrogram of PCR of epidemiologically unrelated strains. Strain numbers are indicated in column A; PCR types are indicated in column B.
- FIG. 3.
Dendrogram of SmaI-PFGE (A) and PCR results (B) from epidemic strains. Strain numbers are indicated on the right side. Strain 84 is the unrelated control.
- FIG. 4.
Agarose gel electrophoresis of PCR-amplified HVR adjacent to the mecA, spa, and coa genes. Strain numbers are indicated on the top of the lanes. Lane M, size marker. Molecular sizes are indicated at the left in base pairs.
- FIG. 5.
Agarose gel electrophoresis of PCR products of clinical unrelated isolates (A) and epidemic strains (B), with strain 84 as a positive amplification control. K, negative control. Strain numbers are indicated at the top of the lanes. Lane M, molecular size marker.
Tables
- TABLE 1.
Comparison of PCR versus PFGE typing resultsa
PCR round and result No of strains tested by PFGE Positive Negative First Positive 32 9 Negative 12 22 Second Positive 35 11 Negative 6 21 ↵a Strains typed by both PFGE and PCR (+/+ strains) were clustered together by PFGE and PCR. Strains typed by neither method (−/− strains) were unrelated by PFGE and PCR. The +/− or −/+ strains were clustered together by PFGE but not by PCR or by PCR but not by PFGE as indicated. In the PCR second run, two strains were not typeable.
