DOI: 10.1128/JCM.41.8.3851-3853.2003
Article Figures & Data
Tables
- TABLE 1.
Primer sets used for detection of the meningococcus-specific insertion sequence IS 1106, the meningococcal capsular transfer gene (ctrA), and the meningococcal sialyltransferase gene (siaD)
Primer (reference) Primer sequence (5′ -3′) Amplicon size(s) (bp) IS1106-F (13) ATT ATT CAG ACC GCC GGC AG IS1106-R TGC CGT CCT GCA ACT GAT GT 331 ctrA-F (11) GCT GCG GTA GGT GGT TCA A ctrA-R TTG TCG CGG ATT TGC AAC TA 111 siaD B-F (11) CTC TCA CCC TCA ACC CAA TGT C siaD B-R TGT CGG CGG AAT AGT AAT AAT GTT 457 siaD C-F (11) GCA CAT TCA GGC GGG ATT AG siaD C-R TCT CTT GTT GGG CTG TAT GGT GTA 442 siaD W-135/Y-F (11) CAA ACG GTA TCT GAT GAA ATG CTG GAA G 698a siaD W-135/Y-R TTA AAG CTG CGC GGA AGA ATA GTG AAA T 438, 260b 16S-F AGA GTT TGA TCA TGG CTC AG 16S-R GGA CTA CCA GGG TAT CTA AT 798 - TABLE 2.
Test characteristics of Gram staining, culture, and PCR of CSF for diagnosis of meningococcal meningitis in 38 infected patients
Test No. of samples Sensitivity (%) Specificity (%) NPVa (%) PPVb (%) Positive Negative Gram staining 25 13 66 100 95 100 Culture 21 17 55 100 93 100 Gram staining culture 33 5 87 100 98 100 PCR 37 1 97 99.6 99.6 97
