Article Figures & Data
Figures
- FIG. 1.
Immunoprecipitation of culture fluids from Vero cells infected with JEV and CHO cells transfected with pcJENS1NS2A (3G8 cells) or pcJENS1 (MF6 cells) with a monoclonal antibody to NS1 (2D5). Samples heated or unheated under nonreduced conditions were run on an 8.5% polyacrylamide gel and detected by silver staining.
- FIG. 2.
Comparison of ELISA reactions obtained with 3 dilutions of capture antibody (rabbit anti-NS1 hyperimmune serum) and 5 dilutions of 3G8 cell culture fluid containing 0.01 to 100 ng of NS1 antigen per ml (corresponding to 0.001 to 10 ng/well) and with highly positive (solid squares), moderately positive (solid circles), low-positive (open diamonds), and negative (open triangles) sera.
- FIG. 3.
Dose-response absorbance curves of highly positive (solid circles), moderately positive (open circles), low-positive (solid triangles), and negative (open triangles) sera. Microplates were sensitized with a 1:10,000 dilution of rabbit anti-NS1 hyperimmune serum and 3 ng of NS1 antigen.
- FIG. 4.
Comparison of the immunostaining and ELISA methods with horse sera positive (48 samples) or negative (47 samples) by immunostaining. The ordinate indicates ELISA values, and the abscissa indicates NS1 antibody titers obtained by the immunostaining method. A dotted line indicates the cutoff value for ELISA (0.122). NS1 antibody titers of less than 1:80 by immunostaining were regarded as negative.
- FIG. 5.
Comparison of the one-dilution method and the endpoint method in ELISA for quantifying NS1 antibodies, using 30 horse serum samples.
- FIG. 6.
Comparison of results obtained in duplicated ELISA tests, using 20 sera positive and 25 sera negative for NS1 antibodies.
- FIG. 7.
Time course of NS1 antibody levels in sera serially collected from four subclinically infected horses during a JE epizootic in Japan.
Tables
- TABLE 1.
Qualitative comparison between ELISA and immunostaining methods for detection of antibodies to JEV NS1, using 95 horse serum samplesa
ELISA antibodies No. of samples with immunostaining antibodies Total Positive Negative Positive 42 8 50 Negative 6 39 45 Total 48 47 95 ↵ a Collected from horses kept in areas in southern and central Japan where JEV is endemic and used in a previous study (13).
