Article Figures & Data
Figures
- FIG. 1.
Neighbor-joining tree of partial gltA sequences. The tree is based on the complete aligment of 374-bp fragments (GenBank accession numbers are indicated in the tree) corresponding to bp 698 to 973 of the partial gltA sequence of B. schoenbuchensis (GenBank accession no. AJ278183 ). Bootstrap values resulting from 1,000 bootstrap trials are indicated for each major branch. The B. vinsonii sequence (GenBank accession no. Z70015 ) that was most divergent from the B. schoenbuchensis sequence was used as an outgroup.
- FIG. 2.
Immunolocalization of B. schoenbuchensis in the midgut lumen of L. cervi. Cryosections of L. cervi (sample 12) were stained with rat anti-B. schoenbuchensis serum and then with Cy3-labeled anti-rat IgG antibodies. Digital images of the specimens were recorded with phase-contrast bright-field optics (top) and with fluorescence excitation and detection with a tetramethyl rhodamine isothiocyanate fluorescence filter set (middle). (Bottom) Overlay of phase-contrast and fluorescence images.
- FIG. 3.
Ultrastructural analysis of B. schoenbuchensis in the midgut lumen of L. cervi. Transmission electron micrographs of thin sections from the midgut of L. cervi (sample 12) were recorded at magnifications of ×2,000 (A) and ×16,000 (B). The image shown in panel B represents an enlargement of the inset in panel A. The electron-dense tissue in the lower part of panel A represents the midgut epithelium. The arrow in panel B indicates a typical aspect of the gram-negative cell wall.
Tables
- TABLE 1.
Origins of L. cervi samples and Bartonella sp. isolates
L. cervi sample Host (deer) Origin of host in 2002 No. of flies collected No. of culture-positive flies/no. tested Bartonella sp. isolate GenBank accession no. 1 Roe Southern Black Forest 2 2/2 Dlf01 AJ564632 9 Roe Southern Black Forest 8 0/4 10 Roe Southern Black Forest 10 2/2 Dlf10 AJ278183 11 Roe Southern Black Forest 1 2/2 Dlf11 AJ278183 12 Roe Southern Black Forest 5 2/2 Dlf12 AJ278183 13 Roe Harz 1 1/1 Dlf13 AJ278183 14 Roe Harz 1 1/1 Dlf14 AJ278183 15 Red Harz 5 2/2 Dlf15 AJ564633 16 Red Harz 2 0/2 17 Red Harz 2 2/2 Dlf17 AJ564634 18 Red Harz 2 0/2 21 Red Schoenbuch Nature Park 3 3/3 Dlf21 AJ564634 22 Red Schoenbuch Nature Park 5 3/3 Dlf22 AJ564635 23 Red Schoenbuch Nature Park 1 1/1 Dlf23 24 Roe Schoenbuch Nature Park 1 1/1 Dlf24 AJ278183
