DOI: 10.1128/JCM.42.2.871-873.2004
Article Figures & Data
Figures
- FIG. 1.
Flow chart of MTT assay. Abbreviations: BC, bacterial control without rifampin; R, tube with 2 μg of rifampin/ml; C, control without bacteria. LJ, Löwenstein-Jensen.
- FIG. 2.
Growth patterns of rifampin-susceptible (n = 37 samples in the first week, 56 in the second week, and 58 in the third week) and rifampin-resistant (n = 6 in the first week and 8 in the subsequent weeks) strains of M. tuberculosis as reflected by RODU values (means ± standard errors) [(RODU = OD570 of rifampin-containing medium)/(OD570 of drug-free medium)]. The difference in RODU values in each week was statistically significant (P < 0.01 [Mann-Whitney test]).
Tables
- TABLE 1.
Contamination rate and time of interpretation of a direct MTT assay for detection of rifampin-resistant M. tuberculosisa
Assay week No. of contaminated samples/total no. of samples tested (%) No. of interpretable samples/total no. of samples tested (%) No. of samples giving the indicated assay resultb MTT 7H10 (indirect) S R S R 1 1/69 (1.4) 43/68 (63) 37 6 2 4/69 (5.8) 64/65 (98.5) 56 8 3 3/69 (4.3) 66/66 (100) 58 8 Total 8/207 (3.9) 69/69 (100) 61 8 61 8
