DOI: 10.1128/JCM.42.3.1220-1223.2004
Article Figures & Data
Tables
- TABLE 1.
Oligonucleotide primers and detection probes for real-time PCR for the simultaneous detection of E. histolytica, G. lamblia, C. parvum and PhHV-1 (internal control)
Target organism and oligonucleotide Oligonucleotide sequence GenBank accession no. or reference E. histolytica Ehd-239F 5′-ATTGTCGTGGCATCCTAACTCA-3′ X64142 , Z49256 Ehd-88R 5′-GCGGACGGCTCATTATAACA-3′ X64142 , Z49256 histolytica-96T VIC-5′-TCATTGAATGAATTGGCCATTT-3′-nonfluorescent quencher X64142 G. lamblia Giardia-80F 5′-GACGGCTCAGGACAACGGTT-3′ M54878 Giardia-127R 5′-TTGCCAGCGGTGTCCG-3′ M54878 Giardia-105T FAM-5′-CCCGCGGCGGTCCCTGCTAG-3′-black hole quencher 1 M54878 C. parvum CrF 5′-CGCTTCTCTAGCCTTTCATGA-3′ 11 CrR 5′-CTTCACGTGTGTTTGCCAAT-3′ 11 Crypto Texas Red-5′-CCAATCACAGAATCATCAGAATCGACTGGTATC-3′-black hole quencher 2 11 PhHV-1 PhHV-267s 5′-GGGCGAATCACAGATTGAATC-3′ 26 PhHV-337as 5′-GCGGTTCCAAACGTACCAA-3′ 26 PhHV-305tq Cy5-5′-TTTTTATGTGTCCGCCACCATCTGGATC-3′-black hole quencher 2 26 - TABLE 2.
Comparison of microscopic examination of modified acid-fast-stained fecal smears and multiplex E. histolytica-G. lamblia-C. parvum real-time PCR for detection of Cryptosporidium infection in an immunocompromised child with complaints of diarrhea
Date of stool collection (day-mo-yr) Microscopy result Multiplex real-time PCR Result Ct value 31-5-2002 − − 12-9-2002 − + 32.7 17-9-2002 − + 28.0 10-2-2003 + + 26.8 18-2-2003 + + 27.9 10-4-2003 − + 37.1 29-4-2003 + + 31.2 13-5-2003 + + 27.8
