Article Figures & Data
Figures
- FIG. 1.
Sequence identification of the B. lusitaniae human isolate (PoHL1). GenBank accession number, AY209179 .
- FIG. 2.
Midpoint-rooted neighbor-joining tree of partial ospA sequences from the isolate obtained in this study (boldface) and GenBank downloads (*). The Kimura two-parameter model was used to estimate the pairwise distances of the aligned nucleotide sequences (MEGA program, version 2.1). Bootstrap values (500 replicates) are given next to the nodes. The scale indicates the estimated number of nucleotide substitutions per sequence position. GenBank accession numbers: A24014 (ACA1), X14407 (B31), X80257 (PBi), X80256 (PBr), A33374 (PKo), Y10837 (PotiB1), Y10838 (PotiB2), Y10839 (PotiB3), AF227319 (Rio2), AF095941 (UK), and AF369948 (VS219).
- FIG. 3.
MseI restriction patterns of B. burgdorferi sensu lato rrf-rrl spacer nested-PCR products amplified from cultured strains. Lanes: 1, PoHL1, human isolate; 2, PoTiBG163, tick isolate, B. garinii; 3, PBi, B. garinii; 4 and 5, PKo and PGau, respectively, B. afzelii; 6, PoTiBV6, tick isolate, B. valaisiana; 7 to 9, B31, IP3, and Vs219, respectively, B. burgdorferi sensu stricto; 10, HO14, B. japonica.
Tables
- TABLE 1.
Strains and GenBank rrf-rrl nucleotide sequences used in this study
Species Strain or isolate Origin rrf-rrl accession no. B. afzelii PGaua Human, Germany NAb PKo Human, Germany NA B. burgdorferi sensu stricto B31 I. scapularis, United States L30127 VS219 Human, France AY032919 IP3a Human, France NA B. garinii Pbia Human, Austria Z77175 PoTiBG4 I. ricinus, Portugal AY463164 PoTiBG20 I. ricinus, Portugal AY463166 PoTiBG86 I. ricinus, Portugal AY463168 PoTiBG163 I. ricinus, Portugal AY463169 B. lusitaniae PoHL1 Human, Portugal AY209179 PoTiBL37c I. ricinus, Portugal AY463167 B. valaisiana PoTiBV6 I. ricinus, Portugal AY463165 B. japonica HO14 I. ovatus, Japan L30125
