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Bacteriology

First Isolation of Borrelia lusitaniae from a Human Patient

M. Collares-Pereira, S. Couceiro, I. Franca, K. Kurtenbach, S. M. Schäfer, L. Vitorino, L. Gonçalves, S. Baptista, M. L. Vieira, C. Cunha
M. Collares-Pereira
1Unidade de Leptospirose e Borreliose de Lyme
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  • For correspondence: mcp@ihmt.unl.pt
S. Couceiro
1Unidade de Leptospirose e Borreliose de Lyme
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I. Franca
2Unidade de Clínica das Doenças Tropicais
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K. Kurtenbach
3Department of Infectious Disease Epidemiology, Imperial College of Science, Technology and Medicine, London, United Kingdom
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S. M. Schäfer
3Department of Infectious Disease Epidemiology, Imperial College of Science, Technology and Medicine, London, United Kingdom
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L. Vitorino
1Unidade de Leptospirose e Borreliose de Lyme
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L. Gonçalves
1Unidade de Leptospirose e Borreliose de Lyme
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S. Baptista
1Unidade de Leptospirose e Borreliose de Lyme
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M. L. Vieira
1Unidade de Leptospirose e Borreliose de Lyme
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C. Cunha
4Unidade de Biologia Molecular, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, Portugal
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DOI: 10.1128/JCM.42.3.1316-1318.2004
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    FIG. 1.

    Sequence identification of the B. lusitaniae human isolate (PoHL1). GenBank accession number, AY209179 .

  • FIG. 2.
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    FIG. 2.

    Midpoint-rooted neighbor-joining tree of partial ospA sequences from the isolate obtained in this study (boldface) and GenBank downloads (*). The Kimura two-parameter model was used to estimate the pairwise distances of the aligned nucleotide sequences (MEGA program, version 2.1). Bootstrap values (500 replicates) are given next to the nodes. The scale indicates the estimated number of nucleotide substitutions per sequence position. GenBank accession numbers: A24014 (ACA1), X14407 (B31), X80257 (PBi), X80256 (PBr), A33374 (PKo), Y10837 (PotiB1), Y10838 (PotiB2), Y10839 (PotiB3), AF227319 (Rio2), AF095941 (UK), and AF369948 (VS219).

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    FIG. 3.

    MseI restriction patterns of B. burgdorferi sensu lato rrf-rrl spacer nested-PCR products amplified from cultured strains. Lanes: 1, PoHL1, human isolate; 2, PoTiBG163, tick isolate, B. garinii; 3, PBi, B. garinii; 4 and 5, PKo and PGau, respectively, B. afzelii; 6, PoTiBV6, tick isolate, B. valaisiana; 7 to 9, B31, IP3, and Vs219, respectively, B. burgdorferi sensu stricto; 10, HO14, B. japonica.

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  • TABLE 1.

    Strains and GenBank rrf-rrl nucleotide sequences used in this study

    SpeciesStrain or isolateOriginrrf-rrl accession no.
    B. afzelii PGauaHuman, GermanyNAb
    PKoHuman, GermanyNA
    B. burgdorferi sensu strictoB31 I. scapularis, United States L30127
    VS219Human, France AY032919
    IP3aHuman, FranceNA
    B. garinii PbiaHuman, Austria Z77175
    PoTiBG4 I. ricinus, Portugal AY463164
    PoTiBG20 I. ricinus, Portugal AY463166
    PoTiBG86 I. ricinus, Portugal AY463168
    PoTiBG163 I. ricinus, Portugal AY463169
    B. lusitaniae PoHL1Human, Portugal AY209179
    PoTiBL37c I. ricinus, Portugal AY463167
    B. valaisiana PoTiBV6 I. ricinus, Portugal AY463165
    B. japonica HO14 I. ovatus, Japan L30125
    • ↵ a Strain cultured and used as a positive control.

    • ↵ b NA, not applicable.

    • ↵ c Sequence identical to that derived from GT058.

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First Isolation of Borrelia lusitaniae from a Human Patient
M. Collares-Pereira, S. Couceiro, I. Franca, K. Kurtenbach, S. M. Schäfer, L. Vitorino, L. Gonçalves, S. Baptista, M. L. Vieira, C. Cunha
Journal of Clinical Microbiology Mar 2004, 42 (3) 1316-1318; DOI: 10.1128/JCM.42.3.1316-1318.2004

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First Isolation of Borrelia lusitaniae from a Human Patient
M. Collares-Pereira, S. Couceiro, I. Franca, K. Kurtenbach, S. M. Schäfer, L. Vitorino, L. Gonçalves, S. Baptista, M. L. Vieira, C. Cunha
Journal of Clinical Microbiology Mar 2004, 42 (3) 1316-1318; DOI: 10.1128/JCM.42.3.1316-1318.2004
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KEYWORDS

Borrelia
Borrelia Infections

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