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Mycology

Comparison of VITEK 2 with ITS2-Fragment Length Polymorphism Analysis for Identification of Yeast Species

Caroline Massonet, Johan Van Eldere, Mario Vaneechoutte, Thierry De Baere, Jan Verhaegen, Katrien Lagrou
Caroline Massonet
1Department of Immunology and Microbiology, Rega Institute
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  • For correspondence: caroline.massonet@med.kuleuven.ac.be
Johan Van Eldere
1Department of Immunology and Microbiology, Rega Institute
2Laboratorium geneeskunde, U.Z. Gasthuisberg, B-3000 Leuven
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Mario Vaneechoutte
3Department of Clinical Microbiology and Immunology, Ghent University Hospital, B-9000 Ghent, Belgium
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Thierry De Baere
3Department of Clinical Microbiology and Immunology, Ghent University Hospital, B-9000 Ghent, Belgium
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Jan Verhaegen
2Laboratorium geneeskunde, U.Z. Gasthuisberg, B-3000 Leuven
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Katrien Lagrou
2Laboratorium geneeskunde, U.Z. Gasthuisberg, B-3000 Leuven
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DOI: 10.1128/JCM.42.5.2209-2211.2004
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Tables

  • TABLE 1.

    Identification results with ID32C, VITEK 2, and ITS2-FLP

    OrganismNo. of isolatesSystemNo. of isolates with the following result:Final identification established by:
    IdentifiedLow discriminationMisidentificationUnidentified
    C. albicans 7ID32C61 (C. sake)Green colonies on chromagar, morphology on cornmeal agar
    VITEK 27
    ITS2-FLP-I7
    ITS2-FLP-BF7
    C. dubliniensis 1ID32C1
    VITEK 21Weak growth at 42°C
    ITS2-FLP-I1
    ITS2-FLP-BF1
    C. glabrata 18ID32C171Pink colonies on chromagar, no pseudohyphae on cornmeal agar, T + M−a
    VITEK 254 (C. magnoliae)3 (C. magnoliae)5Pink colonies on chromagar, no pseudohyphae on cornmeal agar, T + M−
    1 (C. colliculosa)
    ITS2-FLP-I18
    ITS2-FLP-BF162
    C. guilliermondii 3ID32C3
    VITEK 23
    ITS2-FLP-I3
    ITS2-FLP-BF21
    C. krusei 3ID32C3
    VITEK 212Pink colonies on Chromagar plate
    ITS2-FLP-I3
    ITS2-FLP-BF3
    C. parapsilosis 14ID32C14
    VITEK 2122Morphology on cornmeal agar
    ITS2-FLP-I131 (Trichosporon mucoides)Morphology on cornmeal agarb
    ITS2-FLP-BF131 (T. mucoides)Morphology on cornmeal agarb
    C. tropicalis 3ID32C3
    VITEK 23
    ITS2-FLP-I3
    ITS2-FLP-BF3
    C. neoformans 5ID32C5
    VITEK 25
    ITS2-FLP-I5
    ITS2-FLP-BF32
    B. capitatus 2ID32C2
    VITEK 22
    ITS2-FLP-I2
    ITS2-FLP-BF2
    S. cerevisae 4ID32C4
    VITEK 231 (C. colliculosa)Purple colonies on chromagar, morphology on cornmeal agar
    ITS2-FLP-I4
    ITS2-FLP-BF4
    C. palmioleophila 1ID32C1
    VITEK 21
    ITS2-FLP-I1 (C. guilliermondii)Sequencingc
    ITS2-FLP-BF1 (C. guilliermondii)Sequencingc
    Total61ID32C5812
    VITEK 2417310
    ITS2-FLP-I592
    ITS2-FLP-BF5524
    • ↵ a T + M−, trehalose positive, maltose negative.

    • ↵ b Due to a software problem this strain was misidentified. This problem has been resolved, and similar misidentifications are no longer possible.

    • ↵ c C. palmioleophila and C. guilliermondii have identical ITS2 lengths (323 to 324 bp). Differentiation is possible by restriction with RsaI, which yields a 157-bp restriction fragment for C. guilliermondii and no restriction for C. palmioleophila.

  • TABLE 2.

    VITEK 2 reactions leading to misidentification of the strains tested

    No. of misidentified yeastsReference methodVITEK 2 identificationPossible cause of misidentification
    1 S. cerevisiae C. colliculosa No assimilation of d-maltose
    2 C. krusei UnidentifiedNo cleavage of d-glucosidase-4MU
    4 C. glabrata C. magnoliae Assimilation of d-mannitol and d-raffinose
    5 C. glabrata UnidentifiedNo assimilation of histidine arylamidase; extra assimilation test results were positive
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Comparison of VITEK 2 with ITS2-Fragment Length Polymorphism Analysis for Identification of Yeast Species
Caroline Massonet, Johan Van Eldere, Mario Vaneechoutte, Thierry De Baere, Jan Verhaegen, Katrien Lagrou
Journal of Clinical Microbiology May 2004, 42 (5) 2209-2211; DOI: 10.1128/JCM.42.5.2209-2211.2004

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Comparison of VITEK 2 with ITS2-Fragment Length Polymorphism Analysis for Identification of Yeast Species
Caroline Massonet, Johan Van Eldere, Mario Vaneechoutte, Thierry De Baere, Jan Verhaegen, Katrien Lagrou
Journal of Clinical Microbiology May 2004, 42 (5) 2209-2211; DOI: 10.1128/JCM.42.5.2209-2211.2004
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KEYWORDS

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