DOI: 10.1128/JCM.42.5.2209-2211.2004
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Tables
- TABLE 1.
Identification results with ID32C, VITEK 2, and ITS2-FLP
Organism No. of isolates System No. of isolates with the following result: Final identification established by: Identified Low discrimination Misidentification Unidentified C. albicans 7 ID32C 6 1 (C. sake) Green colonies on chromagar, morphology on cornmeal agar VITEK 2 7 ITS2-FLP-I 7 ITS2-FLP-BF 7 C. dubliniensis 1 ID32C 1 VITEK 2 1 Weak growth at 42°C ITS2-FLP-I 1 ITS2-FLP-BF 1 C. glabrata 18 ID32C 17 1 Pink colonies on chromagar, no pseudohyphae on cornmeal agar, T + M−a VITEK 2 5 4 (C. magnoliae) 3 (C. magnoliae) 5 Pink colonies on chromagar, no pseudohyphae on cornmeal agar, T + M− 1 (C. colliculosa) ITS2-FLP-I 18 ITS2-FLP-BF 16 2 C. guilliermondii 3 ID32C 3 VITEK 2 3 ITS2-FLP-I 3 ITS2-FLP-BF 2 1 C. krusei 3 ID32C 3 VITEK 2 1 2 Pink colonies on Chromagar plate ITS2-FLP-I 3 ITS2-FLP-BF 3 C. parapsilosis 14 ID32C 14 VITEK 2 12 2 Morphology on cornmeal agar ITS2-FLP-I 13 1 (Trichosporon mucoides) Morphology on cornmeal agarb ITS2-FLP-BF 13 1 (T. mucoides) Morphology on cornmeal agarb C. tropicalis 3 ID32C 3 VITEK 2 3 ITS2-FLP-I 3 ITS2-FLP-BF 3 C. neoformans 5 ID32C 5 VITEK 2 5 ITS2-FLP-I 5 ITS2-FLP-BF 3 2 B. capitatus 2 ID32C 2 VITEK 2 2 ITS2-FLP-I 2 ITS2-FLP-BF 2 S. cerevisae 4 ID32C 4 VITEK 2 3 1 (C. colliculosa) Purple colonies on chromagar, morphology on cornmeal agar ITS2-FLP-I 4 ITS2-FLP-BF 4 C. palmioleophila 1 ID32C 1 VITEK 2 1 ITS2-FLP-I 1 (C. guilliermondii) Sequencingc ITS2-FLP-BF 1 (C. guilliermondii) Sequencingc Total 61 ID32C 58 1 2 VITEK 2 41 7 3 10 ITS2-FLP-I 59 2 ITS2-FLP-BF 55 2 4 ↵ a T + M−, trehalose positive, maltose negative.
↵ b Due to a software problem this strain was misidentified. This problem has been resolved, and similar misidentifications are no longer possible.
↵ c C. palmioleophila and C. guilliermondii have identical ITS2 lengths (323 to 324 bp). Differentiation is possible by restriction with RsaI, which yields a 157-bp restriction fragment for C. guilliermondii and no restriction for C. palmioleophila.
- TABLE 2.
VITEK 2 reactions leading to misidentification of the strains tested
No. of misidentified yeasts Reference method VITEK 2 identification Possible cause of misidentification 1 S. cerevisiae C. colliculosa No assimilation of d-maltose 2 C. krusei Unidentified No cleavage of d-glucosidase-4MU 4 C. glabrata C. magnoliae Assimilation of d-mannitol and d-raffinose 5 C. glabrata Unidentified No assimilation of histidine arylamidase; extra assimilation test results were positive