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Bacteriology

Rapid and Specific Detection of Escherichia coli Clonal Group A by Gene-Specific PCR

James R. Johnson, Krista Owens, Amee R. Manges, Lee W. Riley
James R. Johnson
1Mucosal and Vaccine Research Center, Minneapolis Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota
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  • For correspondence: johns007@umn.edu
Krista Owens
1Mucosal and Vaccine Research Center, Minneapolis Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota
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Amee R. Manges
2Division of Epidemiology, School of Public Health
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Lee W. Riley
2Division of Epidemiology, School of Public Health
3Division of Infectious Diseases and Immunity, University of California at Berkeley, Berkeley, California
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DOI: 10.1128/JCM.42.6.2618-2622.2004
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ABSTRACT

PCR primers specific for the recently described antimicrobial resistance-associated Escherichia coli clonal group A (CGA), a widespread cause of drug-resistant urinary tract infections in the United States, were devised on the basis of a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., C288T. In comparison with two reference PCR-based fingerprinting methods, ERIC2 PCR and random amplified polymorphic DNA (RAPD) analysis, a PCR assay incorporating the new primers provided 100% sensitivity and 100% specificity for the detection of CGA among 138 diverse clinical and reference E. coli isolates. E. coli reference (ECOR) strain 47 was shown to be a member or a close relative of CGA (by ERIC2 PCR and RAPD analysis, respectively) and yielded a positive assay result. The new CGA-specific PCR assay, which exhibited interlaboratory reproducibility and stability under various experimental conditions, should allow the rapid and specific detection of CGA by any laboratory equipped for diagnostic PCR.

  • Copyright © 2004 American Society for Microbiology
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Rapid and Specific Detection of Escherichia coli Clonal Group A by Gene-Specific PCR
James R. Johnson, Krista Owens, Amee R. Manges, Lee W. Riley
Journal of Clinical Microbiology Jun 2004, 42 (6) 2618-2622; DOI: 10.1128/JCM.42.6.2618-2622.2004

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Rapid and Specific Detection of Escherichia coli Clonal Group A by Gene-Specific PCR
James R. Johnson, Krista Owens, Amee R. Manges, Lee W. Riley
Journal of Clinical Microbiology Jun 2004, 42 (6) 2618-2622; DOI: 10.1128/JCM.42.6.2618-2622.2004
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KEYWORDS

Escherichia coli
polymerase chain reaction

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