Evaluation of a High-Throughput Repetitive-Sequence-Based PCR System for DNA Fingerprinting of Mycobacterium tuberculosis and Mycobacterium avium Complex Strains

IS6110-RFLP patterns of the four major multistrain clusters of M. tuberculosis isolates in Fig. 1. The image was constructed from a single gel. Because some lanes exhibited stronger signals than others, a composite of long exposure and short exposure images was constructed. M. tuberculosis strain H37Ra patterns are shown on each end.

Rep-PCR analysis of M. avium subspecies avium samples. The dendrogram (A) and gel-like images (D) were generated on coded DNA samples by Bacterial Barcodes, Inc. Correct strain identifications (B) and IS1245-RFLP clusters (C) were added by the authors. Duplicate samples are marked with asterisks. NT, not typeable by IS1245-RFLP.

IS1245-RFLP patterns of the four major multistrain clusters of M. avium subspecies avium isolates in Fig. 3. The image was constructed from a single gel as in Fig. 2, except for the two lanes marked with asterisks. DNA from these two strains was incompletely digested in the main gel image of cluster B. Therefore, an image from a second gel was added to better document the similarity between these two strains.