DOI: 10.1128/JCM.42.8.3388-3398.2004
Article Figures & Data
Figures
- FIG. 1.
cps PCR-RFLP analysis of 12 Klebsiella strains. Lanes M, molecular size markers (in base pairs). The C-pattern codes are indicated above the lanes, and the K serotypes and strain names are given in parentheses. Small differences can be observed between patterns C2d and C2e, on the one hand, and between patterns C4a, C4b, and C4c, on the other. C pattern C4f shares only two fragments with the other C patterns of K4 strains.
- FIG. 2.
Overview of the 102 distinct C patterns found during this study. The five C patterns obtained for clinical strains whose K serotypes were initially unknown and which did not exactly match a reference C pattern are given. The patterns obtained with primer rCPS2 (Fig. 3) are not included. C patterns were ordered by similarity by the unweighted pair group method with arithmetic averages procedure, based on the Pearson coefficient, by using a 1% optimization parameter. Pearson coefficients were converted to percentages with BioNumerics software. Uncertain bands, which were weak and which were not taken into account for pattern comparison, are indicated by dashes.
- FIG. 3.
Comparison of the patterns obtained after amplification with the alternative primer, gnd-specific primer rCPS2, with closely matching C patterns obtained with primer rCPS (and BioNumerics software). In most cases, the patterns obtained with primer rCPS2 differed by one or two bands from the corresponding C pattern obtained for strains of the same K serotype. The only exceptions are for K68 strain 1578 and K6 strain 1283; the C pattern of the latter strain nevertheless shares four fragments with pattern C6a.
Tables
- TABLE 1.
Overview of the 246 strains evaluated in this study
Strain(s)a K serotype(s)b C pattern Speciesc Source 1e Source 2f Date(s) Strains SB165, CIP 52.208 1 C1a K. p. subsp. pneumoniae K1 ref. strain A 5054 CIP A215 1 C1a K. p. subsp. pneumoniae Eyelid France, France 1954 CIP 52.144 1 C1a K. p. subsp. pneumoniae Laboratory strain 398 1 C1a K. p. subsp. pneumoniae Sputum Louvain, Belgium 1976 408 1 C1a K. p. subsp. pneumoniae Blood Paris, France 1976 428 1 C1a K. p. subsp. pneumoniae Blood St Etienne, France 1976 440 1 C1a K. p. subsp. pneumoniae Blood Paris, France 1977 467 1 C1a K. p. subsp. pneumoniae Laboratory strain Lyon, France 1977 468 1 C1a K. p. subsp. pneumoniae Trachea Paris, France 1977 503 1 C1a K. p. subsp. pneumoniae Sputum Roanne, France 1977 643 1 C1a K. p. subsp. pneumoniae CSF Paris, France 1980 687 1 C1a K. p. subsp. pneumoniae CSF Créteil, France 1980 744 1 C1a K. p. subsp. pneumoniae Mare, metritis Paris, France 1981 798 1 C1a K. p. subsp. pneumoniae Sputum Paris, France 1982 920 1 C1a K. p. subsp. pneumoniae Pleural fluid Pontarlier, France 1984 926 1 C1a K. p. subsp. pneumoniae Blood Paris, France 1984 SB166, CIP 52.145 2 C2a K. p. subsp. pneumoniae K2 ref. strain B 5055 675 2 C2a K. p. subsp. pneumoniae Vaccine strain Montpellier, France 1980 1594 2 C2a K. p. subsp. pneumoniae Monkey Mulhouse, France 1988 1584 2 C2b K. p. subsp. pneumoniae Blood Briançon, France 1988 777 2 C2c K. p. subsp. pneumoniae Blood Paris, France 1981 34 2 C2d K. p. subsp. pneumoniae Laboratory strain CIP 52.204 2 C2e K. p. subsp. pneumoniae Laboratory strain 35 2 C2e K. p. subsp. pneumoniae Laboratory strain 778 2 C2e K. p. subsp. pneumoniae CSF Paris, France 1981 779 2 C2e K. p. subsp. pneumoniae Abcess Paris, France 1981 SB167, CIP 52.146 3 C3a K. p. subsp. pneumoniae K3 ref. strain C 5046 CIP 52.210T, ATCC 13884T 3 C3a K. p. subsp. rhinoscleromatis Type strain CIP 82.91T, ATCC 13883T 3 C3a K. p. subsp. pneumoniae Type strain ATCC 6908 3 C3a K. p. subsp. rhinoscleromatis Reference strain CIP 54.45 3 C3a K. p. subsp. rhinoscleromatis Nose Vietnam 1954 CIP 54.129 3 C3a K. p. subsp. rhinoscleromatis Rhinoscleroma Vietnam 1954 475 3 C3a K. p. subsp. rhinoscleromatis Sputum Poland 1977 538 3 C3a K. p. subsp. pneumoniae Urine Paris, France 1977 745 3 C3a K. p. subsp. rhinoscleromatis Granuloma Algérie 1981 746 3 C3a K. p. subsp. rhinoscleromatis Rhinoscleroma Algeria 1981 839 3 C3a K. p. subsp. rhinoscleromatis Nose Paris, France 1982 1509 3 C3a K. p. subsp. pneumoniae Feces Paris, France 1988 1635 3 C3a K. p. subsp. pneumoniae Pus Paris, France 1989 1649 3 C3a K. p. subsp. pneumoniae Blood Paris, France 1989 34.81 3 C3a K. p. subsp. rhinoscleromatis Nose France 1981 42.79 3 C3a K. p. subsp. rhinoscleromatis Nose Paris, France 1979 SB1785 3 C3a K. p. subsp. rhinoscleromatis Sputum Poland 1977 SB1793 3 C3a K. p. subsp. rhinoscleromatis Granuloma Wien, Austria 2001 SB168, CIP 52.211T 4 C4a K. p. subsp. ozaenae K4 ref. strain D 5050 ATCC 11296 T 4 C4a K. p. subsp. ozaenae Type strain Indonesia 1930 863 4 C4a K. p. subsp. ozaenae Sputum Lyon, France 1983 1575 4 C4a K. p. subsp. ozaenae Blood Paris, France 1988 1202 4 C4a K. p. subsp. ozaenae Blood Paris, France 1986 1510 4 C4a K. p. subsp. ozaenae Ear pus Paris, France 1988 919 4 C4b K. p. subsp. ozaenae Lung Tours, France 1989 909 4 C4c K. p. subsp. ozaenae Bronchial asp Pontoise, France 1984 1611 4 C4d K. p. subsp. ozaenae Blood Caen, France 1989 748 4 C4e K. p. subsp. ozaenae Nose Algeria 1981 889 4 C4f K. p. subsp. ozaenae Bronchial asp Grenoble, France 1983 CIP 52.212 5 C5a K. p. subsp. ozaenae K5 ref. strain E 5051 189 5 C5a K. p. subsp. pneumoniae Laboratory strain 1973 325 5 C5a K. p. subsp. pneumoniae Human Paris, France 1975 421 5 C5a K. p. subsp. ozaenae Nose Kigali, Rwanda 1976 CIP 52.213 6 C6a K. p. subsp. pneumoniae K6 ref. strain F 5052 CIP 80.42 6 C6b K. p. subsp. pneumoniae Wood Compiègne, France 1979 SB179, CIP 52.205 7 C7a K. p. subsp. pneumoniae K7 ref. strain Aerogenes 4140 1543 7 C7a K. p. subsp. pneumoniae Horse, penis Paris, France 1988 1563 7 C7a K. p. subsp. pneumoniae Horse, penis Paris, France 1989 1583 7 C7a K. p. subsp. pneumoniae Horse Caen, France 1988 1604 7 C7a K. p. subsp. pneumoniae Horse, sperm Paris, France 1988 CIP 52.206 8 C8a K. planticola K8 ref. strain Klebsiella 1015 1574 8 C8b K. p. subsp. pneumoniae Blood Paris, France 1988 SB181, CIP 52.207 9 C9a K. p. subsp. pneumoniae K9 ref. strain Klebsiella 1056 SB182, CIP 52.214 10 C10a K. p. subsp. pneumoniae K10 ref. strain Klebsiella 919 Source 1e Source 2f Date(s) 1141 10 C10b K. p. subsp. pneumoniae Bronchial asp Lyon, France 1986 CIP 52.215 11 C11a K. p. subsp. pneumoniae K11 ref. strain Klebsiella 390 SB184, CIP 52.216 12 C12a K. p. subsp. pneumoniae K12 ref. strain Klebsiella 313 SB185, CIP 52.217 13 C13a K. p. subsp. pneumoniae K13 ref. strain Klebsiella 1470 SB186, CIP 52.218 14 C14a K. planticola K14 ref. strain 138 SB187, CIP 52.219 15 C15a K. p. subsp. pneumoniae K15 ref. strain Mich. 61 SB188, CIP 52.220 16 C16a K. p. subsp. pneumoniae K16 ref. strain 2069/49 1632 16 C16a K. p. subsp. pneumoniae Septic tank Paris, France 1988 SB189, CIP 52.221 17 C17a K. p. subsp. pneumoniae K17 ref. strain 2005/49 SB190, CIP 52.222 18 C18a K. p. subsp. pneumoniae K18 ref. strain 1754/49 SB191, CIP 52.223 19 C19a K. p. subsp. pneumoniae K19 ref. strain 293/50 SB192 20 C20a K. p. subsp. pneumoniae K20 ref. strain 889/50 SB193, CIP 52.225 21 C21a K. p. subsp. pneumoniae K21 ref. strain 1702/49 SB194, CIP 52.226 22 C22,37a K. p. subsp. pneumoniae K22 ref. strain 1996/49 SB195, CIP 52.228 23 C23a K. p. subsp. pneumoniae K23 ref. strain 2812/50 SB196, CIP 52.229 24 C24a K. p. subsp. pneumoniae K24 ref. strain 1680/49 1589 24 C24a K. p. subsp. pneumoniae Blood Paris, France 1988 SB197, CIP 52.230 25 C25a K. p. subsp. pneumoniae K25 ref. strain 2002/49 1581 25 C25b K. p. subsp. pneumoniae Blood Paris, France 1988 1601 25 C25b K. p. subsp. pneumoniae Feces Créteil, France 1988 SB198, CIP 53.6 26 C26a K. oxytoca K26 ref. strain 5884 SB199, CIP 52.232 27 C27a K. p. subsp. pneumoniae K27 ref. strain 6613 SB200, CIP 52.233 28 C28a K. p. subsp. pneumoniae K28 ref. strain 5758 SB317, CIP 52.234 29 C29a K. oxytoca K29 ref. strain 5725y SB318, CIP 52.235 30 C30a K. p. subsp. pneumoniae K30 ref. strain 7824 1569 30 C30a K. p. subsp. pneumoniae Horse, penis Paris, France 1988 1623 30 C30a K. p. subsp. pneumoniae Horse, sperm Saint Lo, France 1988 1553 30 C30b K. p. subsp. pneumoniae Horse, sperm Lyon, France 1989 SB319, CIP 52.231 31 C31a K. p. subsp. pneumoniae K31 ref. strain 6258 SB339 32 C32a K. planticola K32 ref. strain 6837 SB473, CIP 53.8 33 C33a K. p. subsp. pneumoniae K33 ref. strain 6168 SB474, CIP 53.9 34 C34a K. p. subsp. pneumoniae K34 ref. strain 7522 SB475, CIP 53.10 35 C35a K. planticola K35 ref. strain 7444 1516 35 C35b K. p. subsp. pneumoniae Urine Paris, France 1988 1386 35 C35c K. p. subsp. pneumoniae Enterocolitis Montpellier, France 1987 SB476, CIP 53.11 36 C36a K. p. subsp. pneumoniae K36 ref. strain 8306 SB477, CIP 53.12 37 C22,37a K. p. subsp. pneumoniae K37 ref. strain 8238 CIP 53.13 38 C38a K. p. subsp. pneumoniae K38 ref. strain 8414 SB479, CIP 53.14 39 C39a K. planticola K39 ref. strain 7749 585 39 C39b K. p. subsp. pneumoniae Hand St Etienne, France 1979 SB480, CIP 53.15 40 C40a K. p. subsp. pneumoniae K40 ref. strain 8588 SB481 41 C41a K. p. subsp. pneumoniae K41 ref. strain 6177 SB482, CIP 53.17 42 C42a K. p. subsp. pneumoniae K42 ref. strain 1702 SB483, CIP 53.19 43 C43a K. p. subsp. pneumoniae K43 ref. strain 2482 SB484, CIP 53.20 44 C44a K. planticola K44 ref. strain 7730 SB485, CIP 53.21 45 C45a K. p. subsp. pneumoniae K45 ref. strain 8464 SB486, CIP 53.22 46 C46a K. p. subsp. pneumoniae K46 ref. strain 5281 SB487, CIP 53.23 47 C47a K. p. subsp. pneumoniae K47 ref. strain 9682 700 47 C47a K. p. subsp. pneumoniae Wood, tropical Compiègne, France 1980 1659 47 C47b K. p. subsp. pneumoniae Feces Paris, France 1989 SB488, CIP 53.24 48 C48a K. planticola K48 ref. strain 1196 SB489, CIP 52.199 49 C49a K. planticola K49 ref. strain 6115 CIP 52.200 50 C50a K. p. subsp. pneumoniae K50 ref. strain 1303/50 SB491, CIP 52.201 51 C51a K. p. subsp. pneumoniae K51 ref. strain 4715/50 SB492 52 C52a K. p. subsp. pneumoniae K52 ref. strain 5759/50 MGH 78578 52 C52a K. p. subsp. pneumoniae Reference strain 1579 52 C52b K. omithinolytica Bronchial asp Toulouse, France 1988 SB493, CIP 53.26 53 C53a K. planticola K53 ref. strain 1756/51 SB494, CIP 53.27 54 C54a K. planticola K54 ref. strain Stanley SB495 55 C55a K. p. subsp. pneumoniae K55 ref. strain 3985/51 SB496 56 C56a K. planticola K56 ref. strain 3534/51 SB497 57 C57a K. planticola K57 ref. strain 4425/51 SB498 58 C58a K. planticola K58 ref. strain 636/52 94 59 C59a K. oxytoca Unknown Paris, France 1972 SB500 60 C60a K. p. subsp. pneumoniae K60 ref. strain 4463/52 SB501 61 C61a K. p. subsp. pneumoniae K61 ref. strain 5710/52 SB502 62 C62a K. p. subsp. pneumoniae K62 ref. strain 5711/52 1629 62 C62a K. p. subsp. pneumoniae Donkey Mulhouse, France 1988 1513 62 C62b K. p. subsp. pneumoniae Feces Paris, France 1988 199 62 C62c K. oxytoca Bronchial asp Paris, France 1974 SB503 63 C63a K. p. subsp. pneumoniae K63 ref. strain 5845/52 SB504 64 C64a K. p. subsp. pneumoniae K64 ref. strain NCTC 8172 SB505 65 C65a K. terrigena K65 ref. strain SW 4 SB506 66 C66a K. oxytoca K66 ref. strain 438 (3a) SB507 67 C67a K. p. subsp. pneumoniae K67 ref. strain 264 (1) 1187 68 C68a K. oxytoca Wound Paris, France 1986 1344 68 C68a K. oxytoca Blood Bordeaux, France 1986 SB508 69 C69a K. p. subsp. pneumoniae K69 ref. strain 889 SB509 70 C70a K. oxytoca K70 ref. strain 167 SB510 71 C71a K. planticola K71 ref. strain 4349 SB511 72 C72a K. planticola K72 ref. strain 1205 SB512 74 C74a K. oxytoca K74 ref. strain 371 SB513 79 C79a K. planticola K79 ref. strain 325 SB514 80 C80a K. p. subsp. pneumoniae K80 ref. strain 708 SB515 81 C81a K. p. subsp. pneumoniae K81 ref. strain 370 SB516 82 C82a K. p. subsp. pneumoniae K82 ref. strain 3454-70 1037 1,58 C1a K. p. subsp. pneumoniae Horse Pont l’Eveque, France 1985 1056 1,58 C1a K. p. subsp. pneumoniae Horse Caen, France 1985 1570 1,58 C1a K. p. subsp. pneumoniae Mare, metritis Paris, France 1988 607 21,31 C21,31a K. p. subsp. pneumoniae Urine Saint-Etienne, France 1978 1622 22,37 C22,37a K. p. subsp. ozaenae Sputum Mali 1989 683 Acapsular C2e K. p. subsp. pneumoniae Human Lyon, France 1980 1340 Acapsular C3a K. p. subsp. pneumoniae Blood Bordeaux, France 1987 1567 30 C30a K. p. subsp. pneumoniae Horse, penis Paris, France 1988 1609 Acapsular C14a K. p. subsp. pneumoniae Urine Paris, France 1988 SB499 59 No or weak PCR product K. planticola K59 ref. strain 2212/52 1586 2 No or weak PCR product K. p. subsp. pneumoniae Blood Briançon, France 1988 1283 6 No or weak PCR product K. terrigena Carrots Mulhouse, France 1988 1416 6 No or weak PCR product K. terrigena Horse, sperm Nantes, France 1988 1476 Acapsular No or weak PCR product K. p. subsp. pneumoniae Hospital env. Rennes, France 1988 1483 16 No or weak PCR product K. p. subsp. pneumoniae Hospital env. Rennes, France 1988 1485 24 No or weak PCR product K. p. subsp. pneumoniae Hospital env. Rennes, France 1988 1544 7 No or weak PCR product K. p. subsp. pneumoniae Horse, penis Paris, France 1988 1578 68 No or weak PCR product K. oxytoca Blood Paris, France 1988 1346 9 No unique PCR product K. p. subsp. pneumoniae Blood Bordeaux, France 1987 1547 6 No unique PCR product K. p. subsp. pneumoniae Mare, uterus Caen, France 1988 1576 3 No unique PCR product K. p. subsp. pneumoniae Urine Paris, France 1988 775 4 No unique PCR product K. p. subsp. ozaenae Nose Dakar, Senegal 1981 Strains in challenge collection of clinical isolatesd SB54 22, 37 C22,37a K. p. subsp. pneumoniae Blood Seville, Spain 1997-1998 SB52 37 C22,37a K. p. subsp. pneumoniae Blood Seville, Spain 1997-1998 SB1111 24 C24a K. p. subsp. pneumoniae Urine Coimbra, Portugal 1997-1998 SB64 25 C25b K. p. subsp. pneumoniae Blood London, United Kingdom 1997-1998 SB118 27 C27a K. p. subsp. pneumoniae Wound Athens, Greece 1997-1998 SB138 27 C27a K. p. subsp. pneumoniae Wound Athens, Greece 1997-1998 SB153 30 C30a K. p. subsp. pneumoniae Blood Ankara, Turkey 1997-1998 SB162 30 C30a K. p. subsp. pneumoniae Blood Istanbul, Turkey 1997-1998 SB223 30 C30a K. p. subsp. pneumoniae Blood Ankara, Turkey 1997-1998 SB114 30 C30b K. p. subsp. pneumoniae Blood Athens, Greece 1997-1998 SB592 30 C30b K. p. subsp. pneumoniae Blood Athens, Greece 1997-1998 SB93 39 C39b K. p. subsp. pneumoniae Blood Krakow, Poland 1997-1998 SB210 52 C52a K. p. subsp. pneumoniae Urine Lille, France 1997-1998 SB44 62 C62a K. p. subsp. pneumoniae Blood Utrecht, The Netherlands 1997-1998 SB164 35 C-NEW-1 K. p. subsp. pneumoniae Blood Athens, Greece 1997-1998 SB204 22, 37 CNEW-2 K. p. subsp. pneumoniae Blood London, United Kingdom 1997-1998 SB120 NT CNEW-3 K. p. subsp. pneumoniae Blood Genoa, Italy 1997-1998 SB39 NT CNEW-3 K. p. subsp. pneumoniae Blood Genoa, Italy 1997-1998 SB113 24 C24alike-a K. p. subsp. pneumoniae Urine Lille, France 1997-1998 SB158 68 C68alike-a K. p. subsp. pneumoniae Urine Genoa, Italy 1997-1998 03-9138 2 C2a K. p. subsp. pneumoniae Abcess France 2003 03-2455 3 C3a K. p. subsp. rhinoscleromatis Nose Mali 2003 ↵a Entries with two strain names separated by a comma correspond to the 47 duplicates (see Materials and Methods).
↵b NT, nontypeable; two numbers separated by a comma mean that the strain reacted with both antisera.
↵c K. p., K. pneumoniae.
↵d The K serotypes of the clinical strains were determined in this study after cps PCR-RFLP analysis.
↵e ref., reference; CSF, cerebrospinal fluid; asp, aspirate; env., environment.
↵f Where source 1 is a reference strain, source 2 is the original strain name in the reference collection of the WHO reference center for E. coli and Klebsiella.
