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Journal of Clinical Microbiology
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Virology

Real-Time PCR Assay Using Molecular Beacon for Quantitation of Hepatitis B Virus DNA

Simon Siu-Man Sum, Danny Ka-Ho Wong, Man-Fung Yuen, He-Jun Yuan, Jian Yu, Ching-Lung Lai, David Ho, Linqi Zhang
Simon Siu-Man Sum
1Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region
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Danny Ka-Ho Wong
1Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region
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Man-Fung Yuen
1Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region
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He-Jun Yuan
2Department of Medicine, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China
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Jian Yu
3The Aaron Diamond AIDS Research Center, Rockefeller University, New York, New York
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Ching-Lung Lai
1Department of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong Special Administrative Region
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  • For correspondence: hrmelcl@hkucc.hku.hk LZhang@adarc.org
David Ho
3The Aaron Diamond AIDS Research Center, Rockefeller University, New York, New York
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Linqi Zhang
3The Aaron Diamond AIDS Research Center, Rockefeller University, New York, New York
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  • For correspondence: hrmelcl@hkucc.hku.hk LZhang@adarc.org
DOI: 10.1128/JCM.42.8.3438-3440.2004
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    FIG. 1.

    Testing of HBV beacon assay with EUROHEP reference plasmas. Serial ninefold dilutions were made from the EUROHEP standards of subtypes ad and ay. Each sample was analyzed in duplicate in 10 runs. The mean concentration of HBV DNA measured by the HBV beacon assay was plotted against the expected HBV DNA concentration (r = 0.999; P < 0.001).

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    FIG. 2.

    Correlation plot of log10 HBV DNA values obtained with COBAS AMPLICOR HBV Monitor test and HBV beacon assay (r = 0.908; P < 0.001).

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Real-Time PCR Assay Using Molecular Beacon for Quantitation of Hepatitis B Virus DNA
Simon Siu-Man Sum, Danny Ka-Ho Wong, Man-Fung Yuen, He-Jun Yuan, Jian Yu, Ching-Lung Lai, David Ho, Linqi Zhang
Journal of Clinical Microbiology Aug 2004, 42 (8) 3438-3440; DOI: 10.1128/JCM.42.8.3438-3440.2004

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Real-Time PCR Assay Using Molecular Beacon for Quantitation of Hepatitis B Virus DNA
Simon Siu-Man Sum, Danny Ka-Ho Wong, Man-Fung Yuen, He-Jun Yuan, Jian Yu, Ching-Lung Lai, David Ho, Linqi Zhang
Journal of Clinical Microbiology Aug 2004, 42 (8) 3438-3440; DOI: 10.1128/JCM.42.8.3438-3440.2004
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KEYWORDS

hepatitis B virus
Hepatitis B, Chronic
polymerase chain reaction

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