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Mycology

Interlaboratory Comparison of Results of Susceptibility Testing with Caspofungin against Candida and Aspergillus Species

Frank C. Odds, Mary Motyl, Roberto Andrade, Jacques Bille, Emilia Cantón, Manuel Cuenca-Estrella, Amanda Davidson, Christian Durussel, David Ellis, Elyse Foraker, Annette W. Fothergill, Mahmoud A. Ghannoum, Robert A. Giacobbe, Miguel Gobernado, Rosemary Handke, Michel Laverdière, Wendy Lee-Yang, William G. Merz, Luis Ostrosky-Zeichner, Javier Pemán, Sophia Perea, John R. Perfect, Michael A. Pfaller, Laurie Proia, John H. Rex, Michael G. Rinaldi, Juan-Luis Rodriguez-Tudela, Wiley A. Schell, Christine Shields, Deanna A. Sutton, Paul E. Verweij, David W. Warnock
Frank C. Odds
1Aberdeen Fungal Group, University of Aberdeen, Institute of Medical Sciences, Aberdeen AB25 2ZD, United Kingdom
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  • For correspondence: f.odds@abdn.ac.uk
Mary Motyl
2Merck Research Laboratories, Whitehouse Station, New Jersey
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Roberto Andrade
3University of Texas-Houston Medical School, Houston, Texas 77030
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Jacques Bille
4Clinical Microbiology Laboratory, University Hospital, 1011 Lausanne, Switzerland
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Emilia Cantón
5Service of Microbiology, University Hospital La Fe, 46009 Valencia
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Manuel Cuenca-Estrella
6National Centre for Microbiology, Instituto de Salud Carlos III, 28220 Majadahonda, Spain
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Amanda Davidson
1Aberdeen Fungal Group, University of Aberdeen, Institute of Medical Sciences, Aberdeen AB25 2ZD, United Kingdom
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Christian Durussel
4Clinical Microbiology Laboratory, University Hospital, 1011 Lausanne, Switzerland
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David Ellis
7Mycology Unit, Women's and Children's Hospital, Adelaide, Australia
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Elyse Foraker
8 Infectious Disease Laboratory, Christiana Care Health Services, Wilmington, Delaware 19801
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Annette W. Fothergill
9Fungus Testing Laboratory, Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-3900
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Mahmoud A. Ghannoum
10Center for Medical Mycology, Department of Dermatology, Case Western Reserve University, Cleveland, Ohio 44106-5028
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Robert A. Giacobbe
2Merck Research Laboratories, Whitehouse Station, New Jersey
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Miguel Gobernado
5Service of Microbiology, University Hospital La Fe, 46009 Valencia
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Rosemary Handke
7Mycology Unit, Women's and Children's Hospital, Adelaide, Australia
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Michel Laverdière
11Department of Microbiology-Infectious Diseases, Hopital Maisonneuve- Rosemont, Montréal, Quebec H1T 2M4, Canada
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Wendy Lee-Yang
12Mycotic Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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William G. Merz
13The Johns Hopkins Medical Institutions, Baltimore, Maryland 21287-7093
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Luis Ostrosky-Zeichner
3University of Texas-Houston Medical School, Houston, Texas 77030
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Javier Pemán
5Service of Microbiology, University Hospital La Fe, 46009 Valencia
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Sophia Perea
13The Johns Hopkins Medical Institutions, Baltimore, Maryland 21287-7093
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John R. Perfect
14Duke University Medical Center, Durham, North Carolina 27710
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Michael A. Pfaller
15Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242
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Laurie Proia
16Section of Infectious Diseases, Department of Medicine, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612
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John H. Rex
3University of Texas-Houston Medical School, Houston, Texas 77030
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Michael G. Rinaldi
9Fungus Testing Laboratory, Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-3900
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Juan-Luis Rodriguez-Tudela
6National Centre for Microbiology, Instituto de Salud Carlos III, 28220 Majadahonda, Spain
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Wiley A. Schell
14Duke University Medical Center, Durham, North Carolina 27710
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Christine Shields
13The Johns Hopkins Medical Institutions, Baltimore, Maryland 21287-7093
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Deanna A. Sutton
9Fungus Testing Laboratory, Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-3900
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Paul E. Verweij
17Medical Microbiology, UMC St. Radboud, 6500 HB Nijmegen, The Netherlands
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David W. Warnock
12Mycotic Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
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DOI: 10.1128/JCM.42.8.3475-3482.2004
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ABSTRACT

Seventeen laboratories participated in a study of interlaboratory reproducibility with caspofungin microdilution susceptibility testing against panels comprising 30 isolates of Candida spp. and 20 isolates of Aspergillus spp. The laboratories used materials supplied from a single source to determine the influence of growth medium (RPMI 1640 with or without glucose additions and antibiotic medium 3 [AM3]), the same incubation times (24 h and 48 h), and the same end point definition (partial or complete inhibition of growth) for the MIC of caspofungin. All tests were run in duplicate, and end points were determined both spectrophotometrically and visually. The results from almost all of the laboratories for quality control and reference Candida and Aspergillus isolates tested with fluconazole and itraconazole matched the NCCLS published values. However, considerable interlaboratory variability was seen in the results of the caspofungin tests. For Candida spp. the most consistent MIC data were generated with visual “prominent growth reduction” (MIC2) end points measured at 24 h in RPMI 1640, where 73.3% of results for the 30 isolates tested fell within a mode ± one dilution range across all 17 laboratories. MIC2 at 24 h in RPMI 1640 or AM3 also gave the best interlaboratory separation of Candida isolates of known high and low susceptibility to caspofungin. Reproducibility of MIC data was problematic for caspofungin tests with Aspergillus spp. under all conditions, but the minimal effective concentration end point, defined as the lowest caspofungin concentration yielding conspicuously aberrant hyphal growth, gave excellent reproducibility for data from 14 of the 17 participating laboratories.

  • Copyright © 2004 American Society for Microbiology
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Interlaboratory Comparison of Results of Susceptibility Testing with Caspofungin against Candida and Aspergillus Species
Frank C. Odds, Mary Motyl, Roberto Andrade, Jacques Bille, Emilia Cantón, Manuel Cuenca-Estrella, Amanda Davidson, Christian Durussel, David Ellis, Elyse Foraker, Annette W. Fothergill, Mahmoud A. Ghannoum, Robert A. Giacobbe, Miguel Gobernado, Rosemary Handke, Michel Laverdière, Wendy Lee-Yang, William G. Merz, Luis Ostrosky-Zeichner, Javier Pemán, Sophia Perea, John R. Perfect, Michael A. Pfaller, Laurie Proia, John H. Rex, Michael G. Rinaldi, Juan-Luis Rodriguez-Tudela, Wiley A. Schell, Christine Shields, Deanna A. Sutton, Paul E. Verweij, David W. Warnock
Journal of Clinical Microbiology Aug 2004, 42 (8) 3475-3482; DOI: 10.1128/JCM.42.8.3475-3482.2004

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Interlaboratory Comparison of Results of Susceptibility Testing with Caspofungin against Candida and Aspergillus Species
Frank C. Odds, Mary Motyl, Roberto Andrade, Jacques Bille, Emilia Cantón, Manuel Cuenca-Estrella, Amanda Davidson, Christian Durussel, David Ellis, Elyse Foraker, Annette W. Fothergill, Mahmoud A. Ghannoum, Robert A. Giacobbe, Miguel Gobernado, Rosemary Handke, Michel Laverdière, Wendy Lee-Yang, William G. Merz, Luis Ostrosky-Zeichner, Javier Pemán, Sophia Perea, John R. Perfect, Michael A. Pfaller, Laurie Proia, John H. Rex, Michael G. Rinaldi, Juan-Luis Rodriguez-Tudela, Wiley A. Schell, Christine Shields, Deanna A. Sutton, Paul E. Verweij, David W. Warnock
Journal of Clinical Microbiology Aug 2004, 42 (8) 3475-3482; DOI: 10.1128/JCM.42.8.3475-3482.2004
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KEYWORDS

antifungal agents
Aspergillus
Candida
laboratories
Microbial Sensitivity Tests
Peptides
Peptides, Cyclic

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