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Journal of Clinical Microbiology
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Mycology

Identification of Emerging Human-Pathogenic Pythium insidiosum by Serological and Molecular Assay-Based Methods

Nongnuch Vanittanakom, Jitwadee Supabandhu, Chantana Khamwan, Jutarut Praparattanapan, Sophit Thirach, Narawudt Prasertwitayakij, Worawit Louthrenoo, Siri Chiewchanvit, Napaporn Tananuvat
Nongnuch Vanittanakom
1Department of Microbiology
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  • For correspondence: nvanitta@mail.med.cmu.ac.th
Jitwadee Supabandhu
1Department of Microbiology
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Chantana Khamwan
2Central Laboratory, Maharaj Nakorn Chiang Mai Hospital, Chiang Mai University, Chiang Mai, Thailand
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Jutarut Praparattanapan
3Department of Medicine
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Sophit Thirach
1Department of Microbiology
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Narawudt Prasertwitayakij
3Department of Medicine
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Worawit Louthrenoo
3Department of Medicine
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Siri Chiewchanvit
3Department of Medicine
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Napaporn Tananuvat
4Department of Ophthalmology, Faculty of Medicine
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DOI: 10.1128/JCM.42.9.3970-3974.2004
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  • FIG. 1.
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    FIG. 1.

    Colony of P. insidiosum (4 days old) on SGA (pH 6.9).

  • FIG. 2.
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    FIG. 2.

    (A to C) Zoospore production inside the zoosporangium at time intervals of 3 min. (D to F) Rapid release of zoospores.

  • FIG. 3.
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    FIG. 3.

    (A) Biflagellate zoospore. (B) Encysted zoospore. (C) Germinated zoospore.

  • FIG. 4.
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    FIG. 4.

    Single PCR amplification of DNA from four human isolates of P. insidiosum with primers ITSpy1 and ITSpy2 (lanes 1 to 4). The leftmost lane contained a 100-bp DNA ladder marker.

  • FIG. 5.
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    FIG. 5.

    Immunoblot of P. insidiosum antigens. Lane 1, reaction with serum from a pythiosis patient. Lanes 2 to 4 and lane N, reactions with serum from a penicilliosis patient, preimmunized rabbit antiserum, immunized rabbit antiserum, and pooled human sera (from healthy persons), respectively. Relative molecular masses are shown on the left in kilodaltons.

Tables

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  • TABLE 1.

    P. insidiosum isolates recovered from patients with pythiosis and used in this study

    IsolateClinical specimenUnderlying disease of patient
    MMC44P21-1Tissue biopsy from left legThalassemia
    MMC44P21-2Tissue biopsy from right ankleLeukemia with bone marrow transplantation
    MMC45P21-1Craniotomy from brain tissueThalassemia
    MMC45P21-2Corneal specimen from left eyeUnknown
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Identification of Emerging Human-Pathogenic Pythium insidiosum by Serological and Molecular Assay-Based Methods
Nongnuch Vanittanakom, Jitwadee Supabandhu, Chantana Khamwan, Jutarut Praparattanapan, Sophit Thirach, Narawudt Prasertwitayakij, Worawit Louthrenoo, Siri Chiewchanvit, Napaporn Tananuvat
Journal of Clinical Microbiology Sep 2004, 42 (9) 3970-3974; DOI: 10.1128/JCM.42.9.3970-3974.2004

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Identification of Emerging Human-Pathogenic Pythium insidiosum by Serological and Molecular Assay-Based Methods
Nongnuch Vanittanakom, Jitwadee Supabandhu, Chantana Khamwan, Jutarut Praparattanapan, Sophit Thirach, Narawudt Prasertwitayakij, Worawit Louthrenoo, Siri Chiewchanvit, Napaporn Tananuvat
Journal of Clinical Microbiology Sep 2004, 42 (9) 3970-3974; DOI: 10.1128/JCM.42.9.3970-3974.2004
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KEYWORDS

Pythium

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