DOI: 10.1128/JCM.43.11.5477-5482.2005
Article Figures & Data
Tables
- TABLE 1.
katG double mutations in 87 INH-resistant M. tuberculosis isolates from China
Nucleotide no. for the first mutation in katG Amino acid Second mutation in katG No. (%) of isolates with mutation INH MIC range (μg/ml) Additional mutation(s) (no. of isolates) Nucleotide no. Amino acid G→C at position 944 Ser315Thr G→T at position 1388 Arg463Leu 13 (14.9) 1->256 oxyR-ahpC (2), inhA promoter (2), iniA (1) G→A at position 944 Ser315Asn G→T at position 1388 Arg463Leu 1 (1.1) 16 None G→C at position 944 Ser315Thr G→C at position 2053 Gly685Arg 1 (1.1) 2 None G→C at position 944 Ser315Thr A insertion at position 1329 Frameshift 1 (1.1) 64 oxyR-ahpC (1) G→T at position 1388 Arg463Leu G→T at position 1471 Gly491Cys 2 (2.3) 4-8 inhA (1) G→T at position 1388 Arg463Leu CG→TA at position 1543→1544 Arg515Tyr 1 (1.1) 8 None G→T at position 1388 Arg463Leu 64-bp insertion at position 1559 1 (1.1) >256 oxyR-ahpC (1), efpA (1) - TABLE 2.
Nonsynonymous mutations in INH-susceptible and -resistant M. tuberculosis from China
Gene or gene region Nonsynonymous mutation No. of INH-susceptible isolates (n = 50) No. of INH-resistant isolates with indicated INH MIC range (μg/ml) 0.2 ≤ MIC < 1 (n = 24) 1 ≤ MIC < 10 (n = 48) MIC ≥ 10 (n = 15) furA Ser5 0 0 1 1 katG Completed deletion 0 0 0 2 Frameshift 0 0 1 2 Trp90Stop 0 0 1 0 Ser315 0 9 38 9 Arg463 0 11 17 7 Gly491 0 0 2 0 Arg515 0 0 1 0 Gly685 0 0 1 0 inhA promoter −15 C→T 0 2 8 2 −17 G→T 0 0 0 1 inhA Ile21 0 0 1 1 Ile258 0 0 2 0 oxyR-ahpC −9 G→A 0 0 1 1 −10 C→T 0 0 1 0 −12 C→T 0 0 0 2 −15 C→T 0 0 1 0 −39 C→T 0 0 1 3 −48 G→A 0 0 0 1 kasA Met77 0 0 0 1 Leu245 0 0 1 0 Gly269 0 1 0 1 Gly312 8 2 3 1 Ser341 2 2 0 1 Rv0340 Thr143 4 1 1 0 Gly149 5 0 0 0 Val163 0 0 1 0 iniB Frameshift 0 1 1 0 Gly192 2 2 1 1 iniA Arg537 0 1 0 1 His481 3 2 1 1 iniC Frameshift 0 0 1 1 efpA Ile73 4 3 2 1 Glu520 0 0 0 1 - TABLE 3.
INH-resistant M. tuberculosis isolates with mutations in two or more genes
No. of isolates MIC range (μg/ml) Mutation(s)a furA katG inhA promoter inhA oxyR-ahpC kasA Rv0340 iniB iniA iniC efpA 1 4 1559 C Delb,e −15 C→T 1 16 1311 T Insc M771 1 32 Del −9 G→A 1 >256 Del −12 C→T 5 0.5-32 S315T −15 C→T 1 1 S315T I21T 198 T Inse 1 2 S315T −9 G→A 1 2 S315T −39 C→T 1 2 S315T 79 T Ins 1 16 S315T −17 G→T 1 16 S315T I21V 1 16 S315T G269S 98 A Inse 1 0.5 R463L 1 1 R463L L245R 1 2 R463L I258Te G269S R537H 1 4 R463L V1631 1 16 R463L −15 C→T 1 16 S5P R463L −39 C→Te −39 C→T 2 1-2 S315T; R463L −15 C→T 1 2 S315T; R463L −10 C→T 1 32 S315T; R463L −48 G→A R537H 1 64 S315T; 1329 A −39 C→T Ins 1 >256 R463L; 1559 Ins of 64 bpd,e −12 C→T; −39 C→T E520Ve 1 4 R463L; G491Ce I258Te 1 2 S5P −15 C→T ↵ a Amino acid abbreviations: S, Ser; T, Thr; R, Arg; L, Leu; P, Pro; G, Gly; C, Cys; I, Ile; V, Val; M, Met; H, His; E, Glu. Nucleotide abbreviations: A, adenine; C, cytosine; G, guanine, T, thymidine.
↵ b Deletion (Del) of nucleotide C at position 1559.
↵ c Insertion (Ins) of nucleotide T at position 1311.
↵ d Insertion (64 bp) at position 1559.
↵ e Novel mutation.
