DOI: 10.1128/JCM.43.12.5876-5880.2005
Article Figures & Data
Figures
- FIG. 1.
Comparison of nucleic acid sequences of various VP7 types across the assay target region.
- FIG. 2.
Comparison of amino acid sequences of G1 and G2 subtypes identified in the greater Philadelphia area, 1999 to 2000.
Tables
- TABLE 1.
Results obtained from 17 rotavirus strains of known serotype
Strain Serotype Top database hit (strain, accession no.) Avg % homology to top database hit Designated Determined WI79 G1 G1 Wa, M21843 99.0 Wa G1 G1 Wa, M21843 99.8 SC2 G2 G2 95B, U73956 98.9 DS1 G2 G2 No name, L20813 99.6 WI78 G3 G3 YO, D86284 97.0 P G3 G3 Au-17, D86272 98.4 BrB G4 G4 NB111/86, AF161819 98.7 CC4 G4 G4 GR828/86, AF170834 97.8 HCR3 G3 subtype G3 subtype HCR3, L21666 100 VR5-08 G5 G5 IAL-28, L79916 98.1 NG8-038 G8 G8 B37, J04344 98.9 WI61 G9 G9 US1212, AJ250272 95.2 RRV Rhesus G3 Rhesus G3 RRV, M21650 99.2 EDIM Murine G3 Murine G3 EDIM, AF039220 99.8 OSU Porcine G5 Porcine G5 OSU, X04613 99.5 WC3 Bovine G6 Bovine G6 RF, X65940 98.0 B10-01 Bovine G10 Bovine G10 B223, X52650 98.5 - TABLE 2.
Results obtained from five rotavirus vaccine strains (RotaTeq)
Strain Serotype Top database hit (strain, accession no.) Avg % homology to top database hit Designated Determined G1.vaccine G1 G1 Wa, M21843 99.0 G2.vaccine G2 G2 95B, U73956 99.4 G3.vaccine G3 G3 YO, D86284 96.9 G4.vaccine G4 G4 NB111/86, AF161819 98.6 P1.vaccine Bovine G6 Bovine G6 RF, X65940 97.8 - TABLE 3.
Comparison of results from RT-PCR sequence-based typing with monoclonal antibody typing
Monoclonal antibody result RT-PCR sequence result Top database hit (strain, accession no.) % Homology to top database hit G1 G1 K18 , D16319 99.0 G1 G1 TF14 , AF183860 99.5 G1 G1 K18 , D16319 99.0 G1 G1 TF14 , AF183860 99.2 G1 G1 K18 , D16319 98.5 G1 G1 K18 , D16319 98.5 G1 G1 TF14 , AF183860 99.5 G1 G1 K18 , D16319 98.5 G2 G2 TD64 , AF106292 99.5 G3 G3 TK08 , D86281 97.6 G4 G4 GR828/86 , AF170834 97.6 G4 G9 1527, AJ279082 99.5
