Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Clinical Microbiology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Parasitology

Detection and Identification of Enterocytozoon bieneusi and Encephalitozoon Species in Stool and Urine Specimens by PCR and Differential Hybridization

Daan W. Notermans, Ron Peek, Menno D. de Jong, Ellen M. Wentink-Bonnema, René Boom, Tom van Gool
Daan W. Notermans
1Section Parasitology
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: D.W.Notermans@amc.uva.nl
Ron Peek
1Section Parasitology
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Menno D. de Jong
1Section Parasitology
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ellen M. Wentink-Bonnema
1Section Parasitology
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
René Boom
2Section Clinical Virology, Department of Medical Microbiology, Academic Medical Center, Amsterdam
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tom van Gool
1Section Parasitology
3Department of Parasitology, Harbour Hospital, Rotterdam, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/JCM.43.2.610-614.2005
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Tables

  • TABLE 1.

    Serial dilutions of plasmids with inserts of five different microsporidial species after PCR and hybridization with the general microsporidium probe and the specific probe for the respective species

    SpeciesProbeECL signal (LU) with plasmid input in copies/PCRaR2b
    110102103104105106
    E. bieneusi GeneralND3602,49920,47113,59925,09435,4540.87
    SpecificND3973,07816,18520,04733,56546,8970.96
    E. intestinalis General2075972,6048,92925,74424,82334,8080.91
    Specific2437073,02612,96431,26422,03140,8240.86
    E. cuniculi GeneralND3,3475,86026,55938,66433,71647,1890.89
    SpecificND3,0067,24526,00733,09429,03445,1800.89
    E. hellem GeneralND3832,4368,21729,66037,50337,4070.91
    SpecificND4754,01515,41231,66244,12147,3300.97
    V. corneae GeneralND2611,2075,88618,26420,38325,8040.94
    • ↵ a Values represent ECL signal expressed in luminosity units (LU). ND, not done.

    • ↵ b Correlation (squared Pearson's correlation coefficient) between log10-transformed input copy number and ECL signal.

  • TABLE 2.

    Spiked feces with serial dilutions of cultured spores from four different microsporidial species after PCR and hybridization with the general microsporidium probe and the specific probe for the respective species compared with Uvitex 2B stain and light microscopy

    SpeciesaProbeECL signal (LU) with spore input per g of feces (gene copies per PCR) ofb:R2c
    3.5 × 102 (17)3.5 × 103 (1.7 × 102)3.5 × 104 (1.7 × 103)3.5 × 105 (1.7 × 104)
    E. intestinalis General2153,03213,52722,2660.96
    Specific2344,99617,64235,3720.94
    UvitexNeg.Neg.SporadicSome
    E. cuniculi General5624,27726,98727,9670.87
    Specific5644,30535,53835,7860.84
    UvitexNeg.Neg.SporadicFew
    E. hellem General5684,1464,97230,1710.72
    Specific6504,9516,49245,4980.71
    UvitexNeg.Neg.Neg.Sporadic
    V. corneae General2803,7808,07713,5230.99
    UvitexNeg.Neg.Neg.Sporadic
    • ↵ a E. bieneusi spores were not available, as they cannot be maintained in long-term culture.

    • ↵ b Values represent ECL signal expressed in LU.

    • ↵ c Correlation (squared Pearson's correlation coefficient) between log10-transformed input copy number and ECL signal.

  • TABLE 3.

    Two urine samples and PBS after PCR and hybridization with the general microsporidium probe and the specific probes for the respective species

    SampleaProbeECL Signal (LU)
    E. intestinalis (DNA copies/PCR)E. cuniculi (DNA copies/PCR)E. hellem (DNA copies/PCR)
    2.5 × 1062.5 × 1042.5 × 1025.0 × 1065.0 × 1045.0 × 1021.9 × 1061.9 × 1041.9 × 102
    Urine IGeneral23,59018,1423,97229,36818,91346125,55815,805872
    Specific28,46525,4775,18334,35919,99047830,08919,733626
    Urine IIGeneral29,9127,53911,91636,32228,5072,09530,84727,7372,884
    Specific34,11711,92914,12435,58129,3622,75533,58131,2923,971
    PBSGeneral23,45815,46786428,5072,0821,80924,13615,957235
    Specific33,45918,5571,18229,36219,5682,48433,90617,567277
    • ↵ a Samples were spiked with three serial dilutions of cultured spores from each of the three Encephalitozoon species.

PreviousNext
Back to top
Download PDF
Citation Tools
Detection and Identification of Enterocytozoon bieneusi and Encephalitozoon Species in Stool and Urine Specimens by PCR and Differential Hybridization
Daan W. Notermans, Ron Peek, Menno D. de Jong, Ellen M. Wentink-Bonnema, René Boom, Tom van Gool
Journal of Clinical Microbiology Feb 2005, 43 (2) 610-614; DOI: 10.1128/JCM.43.2.610-614.2005

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Journal of Clinical Microbiology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Detection and Identification of Enterocytozoon bieneusi and Encephalitozoon Species in Stool and Urine Specimens by PCR and Differential Hybridization
(Your Name) has forwarded a page to you from Journal of Clinical Microbiology
(Your Name) thought you would be interested in this article in Journal of Clinical Microbiology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Detection and Identification of Enterocytozoon bieneusi and Encephalitozoon Species in Stool and Urine Specimens by PCR and Differential Hybridization
Daan W. Notermans, Ron Peek, Menno D. de Jong, Ellen M. Wentink-Bonnema, René Boom, Tom van Gool
Journal of Clinical Microbiology Feb 2005, 43 (2) 610-614; DOI: 10.1128/JCM.43.2.610-614.2005
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Encephalitozoon
Enterocytozoon
Feces
Nucleic Acid Hybridization
polymerase chain reaction
urine

Related Articles

Cited By...

About

  • About JCM
  • Editor in Chief
  • Board of Editors
  • Editor Conflicts of Interest
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Resources for Clinical Microbiologists
  • Ethics
  • Contact Us

Follow #JClinMicro

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

 

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0095-1137; Online ISSN: 1098-660X