DOI: 10.1128/JCM.43.2.610-614.2005
Article Figures & Data
Tables
- TABLE 1.
Serial dilutions of plasmids with inserts of five different microsporidial species after PCR and hybridization with the general microsporidium probe and the specific probe for the respective species
Species Probe ECL signal (LU) with plasmid input in copies/PCRa R2b 1 10 102 103 104 105 106 E. bieneusi General ND 360 2,499 20,471 13,599 25,094 35,454 0.87 Specific ND 397 3,078 16,185 20,047 33,565 46,897 0.96 E. intestinalis General 207 597 2,604 8,929 25,744 24,823 34,808 0.91 Specific 243 707 3,026 12,964 31,264 22,031 40,824 0.86 E. cuniculi General ND 3,347 5,860 26,559 38,664 33,716 47,189 0.89 Specific ND 3,006 7,245 26,007 33,094 29,034 45,180 0.89 E. hellem General ND 383 2,436 8,217 29,660 37,503 37,407 0.91 Specific ND 475 4,015 15,412 31,662 44,121 47,330 0.97 V. corneae General ND 261 1,207 5,886 18,264 20,383 25,804 0.94 - TABLE 2.
Spiked feces with serial dilutions of cultured spores from four different microsporidial species after PCR and hybridization with the general microsporidium probe and the specific probe for the respective species compared with Uvitex 2B stain and light microscopy
Speciesa Probe ECL signal (LU) with spore input per g of feces (gene copies per PCR) ofb: R2c 3.5 × 102 (17) 3.5 × 103 (1.7 × 102) 3.5 × 104 (1.7 × 103) 3.5 × 105 (1.7 × 104) E. intestinalis General 215 3,032 13,527 22,266 0.96 Specific 234 4,996 17,642 35,372 0.94 Uvitex Neg. Neg. Sporadic Some E. cuniculi General 562 4,277 26,987 27,967 0.87 Specific 564 4,305 35,538 35,786 0.84 Uvitex Neg. Neg. Sporadic Few E. hellem General 568 4,146 4,972 30,171 0.72 Specific 650 4,951 6,492 45,498 0.71 Uvitex Neg. Neg. Neg. Sporadic V. corneae General 280 3,780 8,077 13,523 0.99 Uvitex Neg. Neg. Neg. Sporadic - TABLE 3.
Two urine samples and PBS after PCR and hybridization with the general microsporidium probe and the specific probes for the respective species
Samplea Probe ECL Signal (LU) E. intestinalis (DNA copies/PCR) E. cuniculi (DNA copies/PCR) E. hellem (DNA copies/PCR) 2.5 × 106 2.5 × 104 2.5 × 102 5.0 × 106 5.0 × 104 5.0 × 102 1.9 × 106 1.9 × 104 1.9 × 102 Urine I General 23,590 18,142 3,972 29,368 18,913 461 25,558 15,805 872 Specific 28,465 25,477 5,183 34,359 19,990 478 30,089 19,733 626 Urine II General 29,912 7,539 11,916 36,322 28,507 2,095 30,847 27,737 2,884 Specific 34,117 11,929 14,124 35,581 29,362 2,755 33,581 31,292 3,971 PBS General 23,458 15,467 864 28,507 2,082 1,809 24,136 15,957 235 Specific 33,459 18,557 1,182 29,362 19,568 2,484 33,906 17,567 277 ↵ a Samples were spiked with three serial dilutions of cultured spores from each of the three Encephalitozoon species.