Article Figures & Data
Figures
- FIG. 1.
Microarray results of different clinical Escherichia coli strains with the array tube. Panel A: hybridization with the uropathogenic reference strain E. coli J96. Panel B: hybridization with JF3053, isolated from a case of neonatal meningitis. Panel C: hybridization with the enterohemorrhagic reference strain EDL933. The DNA probe arrangement on the array tube is as follows: A2-4, K88 fimbria (K88); A5-7, K99 fimbria (K99); B2 and C10-11, hemin receptor (chuA); B3-5, 16S rRNA gene (rrs); B6-8, cytolethal distending toxin (cdtB); B9-11, catalase-peroxidase (katP); C2-3, D11, heat-stable toxin (astA); C4-6, colonization factor antigen III (cofA); C7-9, longus pilus (lngA); D2-4, S fimbria (sfaS); D5-7, α-hemolysin (hlyA); D8-10, enterohemorrhagic E. coli hemolysin (ehxA); E2 and F10-11, K5 capsule (kfiB); E3-5, P fimbria (papA); E6-8, S fimbria (sfaA); E9-11, heat-stable toxin (stIA/B); F2-3 and G11, invasion plasmid antigen (ipaH); F4-6, aerobactin (iucC); F7-9, K1 capsule (neuC); G2-4, intimin (eae); G5-7, F1C fimbria (foc); G8-10, type 1 fimbria (fimA); H2 and I10-11, bundle-forming pilus (bfpA); H3-5, colonization factor antigen I (cfa/I); H6-8, cytotoxic necrotizing factor (cnf); H9-11, colonization factor antigen II (cfa/II); I2-3 and J11, heat-labile toxin II (eltIIA); I4-6, aggregative adhesion fimbria (aaf/I); I7-9, ferrichrome-iron receptor (fhuA); J2-4, Shiga toxin 1 (stx1); J5-7, Shiga toxin 2 (stx2); J8-10, heat-labile toxin I (eltIA); columns 1 and 12, biotin-modified DNA sequence dots.
Tables
- TABLE 1.
E. coli isolates analyzed in the study
Strain Origin or clinical settinga K1 agglutination Pattern no.b JF2840 NBM + 12 JF2841 NBM + 13 JF2842 NBM + 15 JF2983 NBM + 5 JF2984 NBM + 7 JF2985 NBM + 11 JF2986 NBM + 17 JF2987 NBM − 20 JF2988 NBM + 7 JF2989 NBM − 23 JF2990 NBM − 20 JF2991 NBM − 21 JF3051 NBM + 7 JF2843 Sepsis + 11 JF2993 Sepsis − 22 JF2994 Sepsis + 18 JF2844 UTI + 13 JF2845 UTI + 2 JF3038 UTI + 7 JF3049 UTI + 10 JF3034 Appendicitis + 11 JF2837 Feces + 5 JF2838 Feces + 1 JF2839 Feces + 16 JF3035 Feces + 14 JF3036 Feces + 14 JF3037 Feces + 7 JF3039 Feces + 7 JF3041 Feces + 7 JF3046 Feces + 7 JF3053 Feces + 19 JF3044 Animal + 3 JF3048 Animal + 13 JF3052 Animal + 7 JF3054 Animal + 3 JF3040 Clinical isolate + 8 JF3042 Clinical isolate + 3 JF3043 Clinical isolate + 4 JF3045 Clinical isolate + 9 JF3050 Clinical isolate + 6 - TABLE 2.
Primers used for amplification of virulence gene probes
Plasmid Gene Primer Sequence Length (nt) Reference Accession no. pJFFECCDT cdtA ECCDT-L GCGGAATTCTCAAGTAGAGGGAGGACCA 598 15 AJ508930 ECCDT-R GCGGAATTCTGGCTTAACAATAGTGGC pJFFECKAT katP ECKAT-L GCGGAATTCACATTTCGTGTGACTGATT 699 9 X89017 ECKAT-R GCGGAATTCATCCGAGGCATATACTTCT pJFFECK88 K88 ECK88-L2 TGCTCTAGAATCGGTGGTAGTATCACTGC 595 11 M25302 ECK88-R2 CCGCTCGAGAACCTGCGACGTCAACAAGA pJFFECK99 K99 ECK99-L GCGGAATTCTGCGACTACCAATGCTTCTG 450 11 M35282 ECK99-R GCGGAATTCTATCCACCATTAGACGGAGC - TABLE 3.
Virulence gene profiles of reference E. coli strainsa
Gene probe E. coli type Enterotoxigenic Enteropathogenic Enteroinvasive Enterohemorrhagic Shiga toxin-producing Enteroaggregative Oropathogenic Neonatal meningitis K-12 34344f NZ3211-94 C9221a IMI100 DS15-1 JF1264 ORG178-03 JF2762 NZ1743-95 NZ1679-94 EDL933 NZ4253-91 NZ2611-99 NZ1470-95 RZ475 J96 536 IHE3034 XL1-Blue Shiga toxin 1 (stx1) • • • Shiga toxin 2 (stx2) • • Heat-labile toxin I (eltlA) • • • • • • Heat-labile toxin II (eltIIA) Aggregative adhesion fimbria (aaf/I) • Ferrichrome-iron receptor (fhuA) • • • • • • • • • • • • • • • • • • Bundle-forming pilus (bfpA) • Colonization factor antigen I (cfa/I) • Cytotoxic necrotizing factor (cnf) • • • Colonization factor antigen II (cfa/II) • • • • Intimin (eaeA) • • • FIC fimbria (foc) • • • • Type I fimbria (fimA) • • • • • • • • • • • • • • Invasion plasmid antigen (ipaH) • Aerobactin (iucC) • • • K1 capsule (neuC) • K5 capsule (kfiB) • P fimbria (papA) • • • S fimbria (sfaS) • • • • Heat-stable toxin (stlA/B) • • • • • • • S fimbria (sfaA) • • • • α-Hemolysin (hlyA) • • • • • Enterohemorrhagic hemolysin (ehxA) • • Heat-stable toxin (astA) • • • • • • • • Colonization factor antigen III (cofA) • Longus filus (lngA) • • • Hemin receptor (chuA) • • • • • • • 16S rRNA gene (rrs) • • • • • • • • • • • • • • • • • • • Cytolethal distending toxin (cdtA) • Catalase (katP) • • K88 fimbria (K88) • • K99 fimbria (K99) • ↵ a Positive signals are indicated by a dot.
- TABLE 4.
Virulence gene patterns of E. coli K1 and related strains analyzed in this studya
Gene probe Pattern no. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 K1 capsule (neuC) • • • • • • • • • • • • • • • • • • • P fimbria (pap) • • • • • • • • • • • • • • • S fimbria (sfaA) • • • • • • • • • S fimbria (sfaS) • • • • • • • • • FIC fimbria (foc) • • • • • • • • • • • • • α-Hemolysin (hlyA) • • • • • • • • Cytotoxic necrotizing factor (cnf) • • • • • Aerobactin (iucC) • • • • • • • • Hemin receptor (chuA) • • • • • • • • • • • • • • • • • • • • • K5 capsule (kfiB) • Heat-stable toxin (astA) • Catalase (katP) • 16S rRNA gene (rrs) • • • • • • • • • • • • • • • • • • • • • • • Type 1 fimbria (fimA) • • • • • • • • • • • • • • • • • • • • • • • Ferrichrom-iron receptor (fhuA) • • • • • • • • • • • • • • • ↵ a Only probes hybridizing with one or more strains are listed. Negative probes are mentioned in the text. Positive signals are indicated by a dot.
