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Epidemiology

Comparative Molecular Analysis of Haemophilus influenzae Isolates from Young Children with Acute Lower Respiratory Tract Infections and Meningitis in Hanoi, Vietnam

Hiroshi Watanabe, Chiharu Kaji, Dang Duc Anh, Phan Le Thanh Huong, Nguyen Thi Hien Anh, Vu Thi Huong, Hoang Vu Mai Phuong, Ngo Thi Thi, Pham Thi Suu, Nguyen Thi Thu Nguyet, Olivia Sebastian Rusizoka, Kiwao Watanabe, Tsuyoshi Nagatake, Kazunori Oishi
Hiroshi Watanabe
1Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
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  • For correspondence: h-wata@net.nagasaki-u.ac.jp
Chiharu Kaji
1Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
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Dang Duc Anh
2National Institute of Hygiene and Epidemiology
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Phan Le Thanh Huong
2National Institute of Hygiene and Epidemiology
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Nguyen Thi Hien Anh
2National Institute of Hygiene and Epidemiology
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Vu Thi Huong
2National Institute of Hygiene and Epidemiology
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Hoang Vu Mai Phuong
2National Institute of Hygiene and Epidemiology
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Ngo Thi Thi
3National Institute of Pediatrics, Hanoi, Vietnam
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Pham Thi Suu
3National Institute of Pediatrics, Hanoi, Vietnam
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Nguyen Thi Thu Nguyet
3National Institute of Pediatrics, Hanoi, Vietnam
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Olivia Sebastian Rusizoka
1Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
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Kiwao Watanabe
1Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
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Tsuyoshi Nagatake
1Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
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Kazunori Oishi
1Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
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DOI: 10.1128/JCM.43.5.2474-2476.2005
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    FIG. 1.

    PFGE patterns of SmaI-digested DNA from 37 H. influenzae isolates from NP of 37 children with acute lower respiratory tract infections. Molecular typing by PFGE demonstrated that 37 H. influenzae strains from the NP had 22 PFGE patterns (A to V), without any predominant pattern. The PFGE patterns of H. influenzae types a, b, and c were different from those of the nontypeable strains.

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    FIG. 2.

    PFGE patterns of SmaI-digested DNA from 48 Hib isolates from the CSF of 44 children with meningitis and the NP of 4 children with acute lower respiratory tract infections. Molecular typing by PFGE demonstrated that the 44 Hib strains from the CSF had nine PFGE patterns (α to ι), with patterns α (22 isolates) and β (12 isolates) predominating. PFGE patterns of 4 Hib strains from the NP were quite different from those of 44 Hib strains from CSF.

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  • TABLE 1.

    Distribution of MICs against 16 antibiotics for H. influenzae strains isolated from nasopharyngeal swabs and cerebrospinal fluid from children in Vietnam

    AntibioticMIC (μg/ml) for isolates from:
    NP (n = 37)CSF (n = 44)
    Range50%90%Range50%90%
    Penicillin G0.5-1281632≤0.004-128232
    Ampicillin0.25-648320.125-3218
    Amoxicillin-clavulanic acid0.25-20.50.50.25-10.250.25
    Cefatrizine2-32482-16416
    Cefuroxime0.5-4140.016-412
    Ceftriaxone≤0.004-0.0320.0080.016≤0.004-0.0320.0080.008
    Cefotaxime0.008-0.1250.0320.032≤0.004-0.1250.0320.063
    Imipenem0.25-4220.25-10.251
    Minocycline0.5-2120.5-211
    Chloramphenicol0.5-16480.5-16816
    Clarithromycin0.25-168164-1688
    Erythromycin0.25-4440.016-824
    Gentamicin1-2120.016-20.52
    Levofloxacin0.016-0.0630.0320.032≤0.004-0.0320.0320.032
    Norfloxacin0.063-0.1250.1250.1250.063-0.1250.0630.125
    Sulfamethoxazole-trimethoprim1-≥128≥128≥1280.032-≥128128≥128
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Comparative Molecular Analysis of Haemophilus influenzae Isolates from Young Children with Acute Lower Respiratory Tract Infections and Meningitis in Hanoi, Vietnam
Hiroshi Watanabe, Chiharu Kaji, Dang Duc Anh, Phan Le Thanh Huong, Nguyen Thi Hien Anh, Vu Thi Huong, Hoang Vu Mai Phuong, Ngo Thi Thi, Pham Thi Suu, Nguyen Thi Thu Nguyet, Olivia Sebastian Rusizoka, Kiwao Watanabe, Tsuyoshi Nagatake, Kazunori Oishi
Journal of Clinical Microbiology May 2005, 43 (5) 2474-2476; DOI: 10.1128/JCM.43.5.2474-2476.2005

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Comparative Molecular Analysis of Haemophilus influenzae Isolates from Young Children with Acute Lower Respiratory Tract Infections and Meningitis in Hanoi, Vietnam
Hiroshi Watanabe, Chiharu Kaji, Dang Duc Anh, Phan Le Thanh Huong, Nguyen Thi Hien Anh, Vu Thi Huong, Hoang Vu Mai Phuong, Ngo Thi Thi, Pham Thi Suu, Nguyen Thi Thu Nguyet, Olivia Sebastian Rusizoka, Kiwao Watanabe, Tsuyoshi Nagatake, Kazunori Oishi
Journal of Clinical Microbiology May 2005, 43 (5) 2474-2476; DOI: 10.1128/JCM.43.5.2474-2476.2005
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KEYWORDS

Haemophilus Infections
Haemophilus influenzae
Meningitis, Bacterial
respiratory tract infections

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