Atypical Serological Response Marked by a Lack of Detectable Anti-gp43 Antibodies in a Patient with Disseminated Paracoccidioidomycosis

(A) immunoblotting of serial patient’s serum samples with a crude P. brasiliensis extract. Lanes: MW, molecular mass standard; C-, blood bank control serum sample; C+, rabbit anti-P. brasiliensis hyperimmune serum; 1 to 5, patient's serum samples from, respectively, 11 September 2001, 24 October 2002, 11 May 2002, 12 December 2002, and 11 March 2004. (B) Immunohistochemistry of a lymph node biopsy taken at the first admission, stained with (a) rabbit anti-crude P. brasiliensis extract or (b) rabbit anti-gp43 antisera.