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Mycobacteriology and Aerobic Actinomycetes

Interpretive Criteria for Use of AccuProbe for Identification of Mycobacterium avium Complex Directly from 7H9 Broth Cultures

Joann L. Cloud, Karen C. Carroll, Samuel Cohen, Clint M. Anderson, Gail L. Woods
Joann L. Cloud
1ARUP Institute for Clinical and Experimental Pathology
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  • For correspondence: cloudjl@aruplab.com
Karen C. Carroll
2Johns Hopkins University, School of Medicine, Baltimore, Maryland
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Samuel Cohen
3ARUP Laboratories
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Clint M. Anderson
3ARUP Laboratories
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Gail L. Woods
1ARUP Institute for Clinical and Experimental Pathology
3ARUP Laboratories
4University of Utah, Department of Pathology, Salt Lake City, Utah
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DOI: 10.1128/JCM.43.7.3474-3478.2005
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  • FIG. 1.
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    FIG. 1.

    Algorithm for identification of Mycobacterium species from 7H9 broth cultures.

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    FIG. 2.

    Summary of results of testing performed in phase 1 of this study.

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  • TABLE 1.

    AccuProbe results for cultures identified as M. avium or M. intracellulare by 16S rRNA gene sequencing

    RLU valueNo. of cultures with the indicated RLU value in:
    Phase 1Phase 2a
    M. aviumM. intracellulareM. aviumM. intracellulare
    <5,0000200
    5,000-9,9992200
    10,000-19,9992501
    20,000-29,9998801
    30,000-49,99981700
    50,000-79,999123003
    80,000-99,9992200
    ≥100,0001422NANA
    • ↵ a NA, not applicable (phase 2 included only isolates with RLU values of <100,000).

  • TABLE 2.

    Mycobacteria other than M. avium or M. intracellulare with MAC AccuProbe results of ≥30,000 RLU

    SpeciesPhase 1Phase 2
    No. of culturesRLU value(s)No. of culturesRLU value(s)
    M. gordonae 533,000, 34,000, 36,000, 40,000, 48,000632,000, 36,000, 36,000, 48,000, 60,000, 72,000
    MTBC172,000534,000, 34,500, 37,000, 41,000, 46,000
    M. chelonae/M. abscessus 232,000, 53,0000NAa
    M. kansasii 139,0000NA
    M. lentiflavum 272,000, 76,0000NA
    M. interjectum 130,0000NA
    M. fortuitum complex0NA130,000
    M. simiae 0NA169,000
    M. terrae complex131,0000NA
    Mycobacterium sp., unable to identify to species level330,000, 31,000, 53,0000NA
    • ↵ a NA, not applicable.

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Interpretive Criteria for Use of AccuProbe for Identification of Mycobacterium avium Complex Directly from 7H9 Broth Cultures
Joann L. Cloud, Karen C. Carroll, Samuel Cohen, Clint M. Anderson, Gail L. Woods
Journal of Clinical Microbiology Jul 2005, 43 (7) 3474-3478; DOI: 10.1128/JCM.43.7.3474-3478.2005

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Interpretive Criteria for Use of AccuProbe for Identification of Mycobacterium avium Complex Directly from 7H9 Broth Cultures
Joann L. Cloud, Karen C. Carroll, Samuel Cohen, Clint M. Anderson, Gail L. Woods
Journal of Clinical Microbiology Jul 2005, 43 (7) 3474-3478; DOI: 10.1128/JCM.43.7.3474-3478.2005
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KEYWORDS

Bacterial Typing Techniques
Molecular Probe Techniques
Mycobacterium avium complex
Mycobacterium avium-intracellulare Infection

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