Interpretive Criteria for Use of AccuProbe for Identification of Mycobacterium avium Complex Directly from 7H9 Broth Cultures

DOI: 10.1128/JCM.43.7.3474-3478.2005

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FIG. 1.
Algorithm for identification of Mycobacterium species from 7H9 broth cultures.
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FIG. 2.
Summary of results of testing performed in phase 1 of this study.

TABLE 1.

AccuProbe results for cultures identified as M. avium or M. intracellulare by 16S rRNA gene sequencing

RLU value	No. of cultures with the indicated RLU value in:
	Phase 1		Phase 2^a
	M. avium	M. intracellulare	M. avium	M. intracellulare
<5,000	0	2	0	0
5,000-9,999	2	2	0	0
10,000-19,999	2	5	0	1
20,000-29,999	8	8	0	1
30,000-49,999	8	17	0	0
50,000-79,999	12	30	0	3
80,000-99,999	2	2	0	0
≥100,000	14	22	NA	NA

↵ a NA, not applicable (phase 2 included only isolates with RLU values of <100,000).

TABLE 2.

Mycobacteria other than M. avium or M. intracellulare with MAC AccuProbe results of ≥30,000 RLU

Species	Phase 1		Phase 2
Species	No. of cultures	RLU value(s)	No. of cultures	RLU value(s)
M. gordonae	5	33,000, 34,000, 36,000, 40,000, 48,000	6	32,000, 36,000, 36,000, 48,000, 60,000, 72,000
MTBC	1	72,000	5	34,000, 34,500, 37,000, 41,000, 46,000
M. chelonae/M. abscessus	2	32,000, 53,000	0	NA^a
M. kansasii	1	39,000	0	NA
M. lentiflavum	2	72,000, 76,000	0	NA
M. interjectum	1	30,000	0	NA
M. fortuitum complex	0	NA	1	30,000
M. simiae	0	NA	1	69,000
M. terrae complex	1	31,000	0	NA
Mycobacterium sp., unable to identify to species level	3	30,000, 31,000, 53,000	0	NA