Article Figures & Data
Tables
- TABLE 1.
Diagnostic categories of patients and specimens
Diagnosis No. of patients No. of pleural fluid specimens No. of pleural tissue specimensa No. of lymph node specimens Tuberculosis 67 53 8 15 Malignancy 11 11 2 0 Amebiasis 4 4 1 0 Congestive heart failure 1 1 0 0 Sarcoidosis 1 0 0 1 Reactive/nonspecific inflammation 3 0 0 3 Total 87 69 11 19 ↵ a Pleural fluid was also analyzed from all of these patients.
- TABLE 2.
Results of culture, AFB smear, histology, cytology, and PCR analyses for 99 specimens from 87 subjects
Method of diagnosis No. of positive specimens/no. of specimens testeda Pleural fluid (n = 69) Pleural tissueb (n = 11) Lymph nodec (n = 19) TB (n = 53) NTB (n = 16) TB (n = 8) NTB (n = 3) TB (n = 15) NTB (n = 4) Conventional culture 0/53 0/16 0/8 0/3 0/15 0/4 USP culture 5/53 0/16 0/8 0/3 1/15 0/4 Conventional smear 1/53 0/16 1/8 0/3 1/15 0/4 USP smear 14/53 0/16 1/8 0/3 1/15 0/4 Conventional smear and culture (combined) 1/53* 0/16 1/8 0/3 1/15 0/4 USP smear and culture (combined) 19/53*, ** 0/16 1/8 0/3 2/15 0/4 Tissue histology ND ND 7/8 0/3 9/9† 0/3† FNA cytology 53/53 0/16 ND ND 12/12‡ 0/3‡ Tissue histology and FNA cytology (combined) 53/53 0/16 7/8 0/3 15/15 0/4 devR PCR 40/53 1/16 5/8 0/3 7/15 1/3d IS6110 PCR 40/53 1/16 6/8 0/3 9/15 1/4e devR and IS6110 PCR (combined) 44/53** 1/16e 7/8 0/3 10/15 1/4 USP smear, culture, and PCR (combined) 46/53 1/16 7/8 0/3 10/15 1/4 ↵ a TB, tuberculosis subjects; NTB, nontuberculosis subjects; ND, not done. *, P < 0.0001 (comparison between combination of conventional smear and culture and combination of USP smear and culture); **, P < 0.0001 (comparison between combination of USP smear and culture and combination of devR and IS6110 PCR); †, seven specimens were not analyzed by histology; ‡, four specimens were not analyzed by cytology.
↵ b These subjects were also assessed by examination of pleural fluid.
↵ c One subject was assessed by examination of pleural fluid, pleural tissue, and lymph node.
↵ d One specimen was not analyzed by devR PCR.
↵ e The PCR false-positive result in the lymph node group was from a patient with sarcoidosis, and that in the pleural fluid group was from a malignant effusion.
- TABLE 3.
Performance of PCR in diagnosis of extrapulmonary tuberculosis
Parametera Pleural fluid (n = 69) Pleural tissue (n = 11) Lymph node (n = 19) devR IS6110 devR and IS6110 combined devR IS6110 devR and IS6110 combined devR IS6110 devR and IS6110 combined Sensitivity (%) 75.5 75.5 83 62.5 75 87.5 46.7 60 66.7 Specificity (%) 93.8 93.8 93.8 100 100 100 66.7 75 75 PPV (%) 97.6 97.6 97.8 100 100 100 87.5 90 90.9 NPV (%) 53.6 53.6 62.5 50 60 75 25 33.3 37.5 Efficiency (%) 79.7 79.7 85.5 72.7 81.8 90.9 47.4 63.2 68.4 ↵ a PPV, positive predictive value; NPV, negative predictive value.
