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Journal of Clinical Microbiology
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Bacteriology

Novel PCR-Restriction Fragment Length Polymorphism Analysis for Rapid Typing of Staphylococcal Cassette Chromosome mec Elements

Jin Ah Yang, Dae Won Park, Jang Wook Sohn, Min Ja Kim
Jin Ah Yang
1Graduate School of Life Science and Biotechnology
2Research Institute of Emerging Infectious Diseases
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Dae Won Park
2Research Institute of Emerging Infectious Diseases
3Division of Infectious Diseases, College of Medicine, Korea University, Seoul, South Korea
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Jang Wook Sohn
2Research Institute of Emerging Infectious Diseases
3Division of Infectious Diseases, College of Medicine, Korea University, Seoul, South Korea
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Min Ja Kim
2Research Institute of Emerging Infectious Diseases
3Division of Infectious Diseases, College of Medicine, Korea University, Seoul, South Korea
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  • For correspondence: macropha@korea.ac.kr
DOI: 10.1128/JCM.44.1.236-238.2006
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ABSTRACT

We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV.

  • Copyright © 2006 American Society for Microbiology
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Novel PCR-Restriction Fragment Length Polymorphism Analysis for Rapid Typing of Staphylococcal Cassette Chromosome mec Elements
Jin Ah Yang, Dae Won Park, Jang Wook Sohn, Min Ja Kim
Journal of Clinical Microbiology Jan 2006, 44 (1) 236-238; DOI: 10.1128/JCM.44.1.236-238.2006

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Novel PCR-Restriction Fragment Length Polymorphism Analysis for Rapid Typing of Staphylococcal Cassette Chromosome mec Elements
Jin Ah Yang, Dae Won Park, Jang Wook Sohn, Min Ja Kim
Journal of Clinical Microbiology Jan 2006, 44 (1) 236-238; DOI: 10.1128/JCM.44.1.236-238.2006
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KEYWORDS

Bacterial Proteins
Bacterial Typing Techniques
Chromosomes, Bacterial
polymerase chain reaction
Recombinases
Staphylococcus aureus

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