Characteristics of Staphylococcus aureus Strains Isolated in Poland in 1996 to 2004 That Were Deficient in Species-Specific Proteins

Agnieszka Luczak-Kadlubowska; Jolanta Krzyszton-Russjan; Waleria Hryniewicz

doi:10.1128/JCM.01164-06

DOI: 10.1128/JCM.01164-06

Article Figures & Data

Tables

TABLE 1.

A. Genotypic and phenotypic characteristics of clumping factor- or coagulase-deficient S. aureus strains

Strain group^a	PFGE type (n^b)	PFGE subtype (n^b)	Genotype					Phenotype (n^b)			Clone characteristics
Strain group^a	PFGE type (n^b)	PFGE subtype (n^b)	ST	SCCmec type	Presence of clfA, clfB, coa, spa, and nuc genes (n^b)	Clonal complex	agr group	Methicillin resistance phenotype	Glycopeptide resistance phenotype^c	Delta-hemolysin production^d	Relatedness to Polish and international S. aureus clones (reference[s])^e	Center code^f	Yr or period of isolation
1	A (1)	A1 (1)	45	IV	+ (4)	CC45	I	MRSA	GSSA	+	HeMRSA-Pol4 (23), Berlin clone (36)	35	2001
	A (3)	A4 (2), A13 (1)	45			CC45	I	MSSA	GSSA	+		7, 19	1999-2004
	B (34)	B5 (16), B8, B46, B47, B48 (3), B49, B50 (4), B51, B52, B53, B54, B55, B56, B57	239, 241	III, IIIB, IIIC	+ (34)	CC8	I	MRSA	GSSA; hGISA (2)	+ (32); − (2)	HoMRSA-Pol1 (23), Hungarian clone (9)	1, 3, 6, 14, 19, 20, 22, 25, 26, 27, 31 40, 41	1997-2003
	C (2)	C4	239	III	+ (2)	CC8	I	MRSA	GSSA	+	He/HoMRSA-Pol1 (23), Brazilian clone (2)	1	1997
	K (2)	K17, K18 (2)	239, 254		+ (5)	CC8	I	MSSA	GSSA	+		26, 41	1998-2001
	K (3)	K19, K21	239	IIIC	+ (3)	CC8	I	MRSA	GSSA	+	HoMRSA-Pol3 (23)	5, 25	1999, 2003
	R (3)	R1	15		+ (3)	CC15	II	MSSA	GSSA	+	Polish MSSA clone	15, 23, 37	1996, 1999, 2002
	N (1)	N9	30		+	CC30	III	MSSA	GSSA	+	HeMRSA-Pol3 (23), SWP⁴ clone (36, 49)	10	1997
	F (1)	F14	5	IV	+	CC5	II	MRSA	GSSA	+	HeMRSA-Pol1 (23), pediatric MRSA clone (23, 33)	35	2001
	G (1)	G1	80	IV	+	Singleton	III	MRSA	GSSA	+	HeMRSA-Pol2 (23), Mediterranean clone (1, 17)	7	1996
	RR (1)	RR1	121		+	CC51	IV	MSSA	GSSA	+		16	1999
	Y (1)	Y2	395		+	Singleton	I	MSSA	GSSA	+	Sporadic	35	1999
	ZZ (1)	ZZ1 (1)	7		+	Singleton	I	MSSA	GSSA	+	Sporadic	4	2002
2	D (30)	D1, D6 (3), D7 (23), D27, D30 D32	247	IA	+ (30)	CC8	I	MRSA	hGISA (23); GSSA	− (22), + (8)	HoMRSA-Pol2 (23), Iberian clone (33)	4, 5, 8, 9,10, 11, 12, 13, 15, 19, 21, 24, 28, 29, 30, 31, 32, 33, 34, 35	1996-2004
	N (1)	N9 (1)	30		+	CC30	III	MSSA	GSSA	+	HeMRSA-Pol3 (23), SWP⁴ clone (36, 49)	36	1998
	R (3)	R1, R2, R3	15		+ (3)	CC15	II	MSSA	GSSA	+	Polish MSSA clone	38, 35	2002, 2000 1996
	K (1)	K23	239	III	+	CC8	I	MRSA	hTISA	−	HoMRSA-Pol3 (23)	2	1998
	WW(1)	XX1	707		+	Singleton	III	MSSA	GSSA	+	Sporadic	23	1996
	YY (1)	YY1	182		+	Singleton	I	MSSA	GSSA	+	Sporadic	10	1997
3	A (1)	A1 (1)	45	IA	+	CC45	I	MRSA	GSSA	+	HoMRSA-Pol4 (23), Berlin clone (36)	38
	D (3)	D30, D7 (2)	247	IA	+	CC8	I	MRSA	hTISA (1), GSSA (1)	− (1), + (1)	HoMRSA-Pol2 (23), Iberian clone (33)	5, 31
	R (3)	R1	15		+ (3)	CC15	II	MSSA	GSSA	+	Polish MSSA clone	13, 7, 18
	N (3)	N8, N10, N11	30		+	CC30	III	MSSA	GSSA	+	HeMRSA-Pol3 (23), SWP clone (36, 49)	16, 38
	XX (3)	XX1	1		+	CC1	II	MSSA	GSSA	+		7, 35, 27
	T (1)	T1	1		+	CC1	III	MSSA	GSSA	+		35
	K (1)	K22	239		+	CC8	I	MSSA	GSSA	+		17
	RR (1)	RR2	121		+	CC51	IV	MSSA	GSSA	+	Sporadic	35
	NY (1)	NY1	5	I	+	CC5	II	MRSA	GSSA	+	EMRSA-3 (36)	16
	Y (1)	Y1	395		+	Singleton	II	MSSA	GSSA	+	Sporadic	12
	X (1)	X2	446		+	Singleton	III	MSSA	GSSA	+	Sporadic	7

↵ a Group 1, based on phenotypical tests: CF(+), COA(−), n = 54; NUC(−), n = 32; NUC(+), n = 22; MAN(−), n = 1; MAN(+), n = 53. Group 2, based on phenotypical tests: CF(−), COA(+), n = 37; NUC(−) n = 1; NUC(+), n = 36; MAN(−), n = 1; MAN(+), n = 36. Group 3, based on phenotypical tests: CF(+), delayed coagulase, COA(+), n = 19; NUC(−), n = 10; NUC(+), n = 10; MAN(−), n = 0; MAN(+), n = 19.
↵ b n, number of isolates.
↵ c The PAP method was used to confirm the hGISA/hTISA phenotype.
↵ d +, enhanced zone of hemolysis created by the interaction of beta-hemolysin of ATCC 25923 and delta-hemolysin of the test strain; −, lack of enhanced zone of hemolysis (lack of delta-hemolysin production).
↵ e HeMRSA and HoMRSA are Polish clones. SWP, Southwest Pacific.
↵ f Center code number reflects the locations of Polish cities or foreign countries of origin of isolates, listed here in alphabetical order: 1, Bielsko Biala; 2, Bulgarian; 3, Bydgoszcz; 4, Bytom; 5, Czestochowa; 6, Gdansk; 7, Grajewo; 8, Hungary; 9, Kalisz; 10, Konin; 11, Koszalin; 12, Krakow1; 13, Krakow2; 14, Lomza; 15, Makow Maz.; 16, Mlawa; 17, Nowa Sol; 18, Olsztyn; 19, Otwock; 20, Poznan; 21, Rzeszow; 22, Siemianowice Sl.; 23, Sieradz; 24, Slovenia; 25, Sochaczew; 26, Suwalki; 27, Szczecin; 28, Warsaw, Waw1; 29, Waw2; 30, Waw3; 31, Waw4; 32, Waw5; 33, Waw6; 34, Waw7; 35, Waw8; 36, Waw9; 37, Wejherowo; 38, Wolomin; 39, Zabrze; 40, Zamosc; 41, Zielona Gora.