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Bacteriology

Performance of the Gen-Probe Transmission-Mediated Amplification Research Assay Compared to That of a Multitarget Real-Time PCR for Mycoplasma genitalium Detection

Justin Hardick, Julie Giles, Andrew Hardick, Yu-Hsiang Hsieh, Thomas Quinn, Charlotte Gaydos
Justin Hardick
1Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland
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  • For correspondence: piccalo13@hotmail.com
Julie Giles
1Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland
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Andrew Hardick
1Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland
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Yu-Hsiang Hsieh
2Johns Hopkins University Department of Emergency Medicine, Baltimore, Maryland
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Thomas Quinn
1Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland
3National Institute of Allergy and Infectious Diseases, National Institutes of Health Bethesda, Maryland
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Charlotte Gaydos
1Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland
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DOI: 10.1128/JCM.44.4.1236-1240.2006
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This article has a correction. Please see:

  • Performance of the Gen-Probe Transcription-Mediated Amplification Research Assay Compared to That of a Multitarget Real-Time PCR for Mycoplasma genitalium Detection
    - June 06, 2006

ABSTRACT

Mycoplasma genitalium (MG) can cause nongonococcal urethritis and is potentially associated with urethritis, endometritis, and cervicitis. Several assays have been developed to detect MG. Molecular amplification assays for organism detection can be problematic due to the potential for false-positive and false-negative results. Confirmatory testing is often required in these situations, requiring additional time and resources. Use of multigene targets could integrate both detection and verification at lower cost. Utilizing two targets, the MgPa adhesion gene and the 16S rRNA gene, a multitarget real-time (MTRT) PCR for the detection of MG was developed. Samples from patients attending sexually transmitted disease clinics were collected in duplicate. Urine samples from males (n = 286) and self-collected vaginal swabs from females (n = 321) were analyzed by MTRT PCR for MG and the Gen-Probe transmission-mediated amplification (TMA) assay, which targets MG rRNA for detection (TMA-MG research use only). Utilizing the criteria of any two targets being positively amplified, the MTRT PCR had a sensitivity and specificity of 91.8% (101 positive samples/110 samples tested) and 99.5% (495/497), respectively, with a positive predictive value (PPV) of 98.1% (101/103) and a negative predictive value (NPV) of 98.2% (495/504). The Gen-Probe TMA-MG assay had a sensitivity, specificity, PPV, and NPV of 98.1% (108/110), 98.1% (488/497), 92.3% (108/117), and 99.5% (488/490), respectively. Comparison between the MTRT PCR and TMA-MG assay by kappa statistic analysis indicated that an overall kappa value was 0.941 (95% confidence interval, 0.907 and 0.976). Both assays demonstrated accuracy in the detection of MG from urine samples from male patients and self-collected vaginal swabs from female patients.

  • Copyright © 2006 American Society for Microbiology
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Performance of the Gen-Probe Transmission-Mediated Amplification Research Assay Compared to That of a Multitarget Real-Time PCR for Mycoplasma genitalium Detection
Justin Hardick, Julie Giles, Andrew Hardick, Yu-Hsiang Hsieh, Thomas Quinn, Charlotte Gaydos
Journal of Clinical Microbiology Apr 2006, 44 (4) 1236-1240; DOI: 10.1128/JCM.44.4.1236-1240.2006

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Performance of the Gen-Probe Transmission-Mediated Amplification Research Assay Compared to That of a Multitarget Real-Time PCR for Mycoplasma genitalium Detection
Justin Hardick, Julie Giles, Andrew Hardick, Yu-Hsiang Hsieh, Thomas Quinn, Charlotte Gaydos
Journal of Clinical Microbiology Apr 2006, 44 (4) 1236-1240; DOI: 10.1128/JCM.44.4.1236-1240.2006
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KEYWORDS

Mycoplasma Infections
Mycoplasma genitalium
polymerase chain reaction

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