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Mycobacteriology and Aerobic Actinomycetes

PCR Amplification of the IS6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis

Ramadass Balamurugan, Subramanian Venkataraman, K. R. John, Balakrishnan S. Ramakrishna
Ramadass Balamurugan
1Departments of Gastrointestinal Sciences
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Subramanian Venkataraman
1Departments of Gastrointestinal Sciences
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K. R. John
2Community Health, Christian Medical College, Vellore, India
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Balakrishnan S. Ramakrishna
1Departments of Gastrointestinal Sciences
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  • For correspondence: rama@cmcvellore.ac.in
DOI: 10.1128/JCM.44.5.1884-1886.2006
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    FIG. 1.

    Electrophoresis of PCR products on a 2% agarose gel (top panels and bottom right panel) or PCR product digest on a 3% agarose gel (bottom left panel). In each panel, lane M is the molecular weight marker. (Top left) Lanes 1 to 11 are DNA isolated from feces of control subjects. Lane 12 shows amplification of a 123-bp fragment from the positive-control DNA. (Bottom left) Digested PCR products, showing 66- and 57-bp fragments. Lanes 1 to 6, results from intestinal TB patients; lanes 7 and 8, results from pulmonary TB patients. Lane 9 is the positive control. (Top right) Lanes 1 to 11 show results from various intestinal TB patients, while lane 12 is a positive control. (Bottom right) Lanes 1 to 11 show results from patients with smear-positive pulmonary TB, while lane 12 shows the positive control.

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  • TABLE 1.

    Outcome of fecal PCR for IS6110 of M. tuberculosis in the various groups studied

    ParameterValue for group
    Intestinal TBControlSputum-positive pulmonary TBTreated intestinal TB
    Fecal PCR positive (no. of samples)160120
    Fecal PCR negative (no. of samples)23028
    Sensitivity (%)88.888.8
    Specificity (%)100100
    Negative predictive value (%)93.793.7
    Positive predictive value (%)100100
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PCR Amplification of the IS6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis
Ramadass Balamurugan, Subramanian Venkataraman, K. R. John, Balakrishnan S. Ramakrishna
Journal of Clinical Microbiology May 2006, 44 (5) 1884-1886; DOI: 10.1128/JCM.44.5.1884-1886.2006

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PCR Amplification of the IS6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis
Ramadass Balamurugan, Subramanian Venkataraman, K. R. John, Balakrishnan S. Ramakrishna
Journal of Clinical Microbiology May 2006, 44 (5) 1884-1886; DOI: 10.1128/JCM.44.5.1884-1886.2006
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KEYWORDS

Mycobacterium tuberculosis
polymerase chain reaction
Tuberculosis, Gastrointestinal

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