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Mycology

Amplification of the Hyphal Wall Protein 1 Gene To Distinguish Candida albicans from Candida dubliniensis

Orazio Romeo, Cosimo Racco, Giuseppe Criseo
Orazio Romeo
Department of Microbiological, Genetic, and Molecular Sciences, University of Messina, Salita Sperone, 31 98166 Messina, Italy
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Cosimo Racco
Department of Microbiological, Genetic, and Molecular Sciences, University of Messina, Salita Sperone, 31 98166 Messina, Italy
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Giuseppe Criseo
Department of Microbiological, Genetic, and Molecular Sciences, University of Messina, Salita Sperone, 31 98166 Messina, Italy
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  • For correspondence: gcriseo@unime.it
DOI: 10.1128/JCM.00125-06
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    FIG. 1.

    Differentiation of C. dubliniensis and C. albicans by the PCR/RFLP method. Lanes 1 and 4, PCR products of C. dubliniensis CBS 7987; lanes 2 and 3, PCR products of C. albicans ATCC 10231; lane 5, PCR products of C. dubliniensis CBS 7987 digested with the BamHI restriction enzyme; lane 6, PCR products of C. albicans ATCC 10231 digested with the BamHI restriction enzyme; lane M, molecular size marker.

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  • TABLE 1.

    Comparison of phenotypic and molecular methods to differentiate C. albicans from C. dubliniensis

    Species and strainOriginLength of PCR product (bp)Length of RLFP product fragments (bp)Appearance of colony on Pal's agarColor of colony on TTC agarc
    C. albicans ATCC 10231aReference straina1,180793, 387SmoothPale pink to whitish
    C. albicans L 30390Ascitic liquid1,180793, 387SmoothPale pink to whitish
    C. albicans L 18907Ascitic liquid1,180793, 387SmoothPale pink to whitish
    C. albicans T 215123Oral swab1,180793, 387SmoothPale pink to whitish
    C. albicans T 25780Oral swab1,180793, 387SmoothPale pink to whitish
    C. albicans T 30378Oral swab1,180793, 387SmoothNot grown
    C. albicans T 215140Oral swab1,180793, 387SmoothPale pink to whitish
    C. albicans T 25781Oral swab1,180793, 387SmoothPale pink to whitish
    C. albicans T-V1Oral swab1,180793, 387SmoothPale pink to whitish
    C. albicans TF 29361Wound swab1,180793, 387Hyphal fringePale pink to whitish
    C. albicans TR 29562Rectal swab1,180793, 387SmoothPale pink to whitish
    C. albicans E 18894Sputum1,180793, 387SmoothPale pink to whitish
    C. albicans E 30384Sputum1,180793, 387SmoothPale pink to whitish
    C. albicans E 29351Sputum1,180793, 387SmoothNot grown
    C. albicans E 29459Sputum1,180793, 387SmoothPale pink to whitish
    C. albicans E 18522Sputum1,180793, 387Hyphal fringePale pink to whitish
    C. albicans AG 29355Gastric aspirate1,180793, 387SmoothPale pink to whitish
    C. albicans A 29463Gastric aspirate1,180793, 387SmoothPale pink to whitish
    C. albicans MAL 012Vaginal swab1,180793, 387SmoothPale pink to whitish
    C. albicans MAL 139Vaginal swab1,180793, 387SmoothPale pink to whitish
    C. albicans S 28274Blood1,180793, 387SmoothPale pink to whitish
    C. dubliniensis CBS 7987bReference strainb930930Hyphal fringeRed to maroon
    C. dubliniensis V3Oral swab930930Hyphal fringeRed to maroon
    C. dubliniensis V4Oral swab930930Hyphal fringeRed to maroon
    C. dubliniensis V5Oral swab930930Hyphal fringeRed to maroon
    C. dubliniensis CD33Oral swab930930Hyphal fringeRed to maroon
    C. dubliniensis CD36Oral swab930930Hyphal fringeRed to maroon
    C. dubliniensis CD519Oral swab930930Hyphal fringeRed to maroon
    C. dubliniensis CAN6Oral swab930930Hyphal fringeRed to maroon
    C. dubliniensis 05-87hOral swab930930Hyphal fringeRed to maroon
    C. dubliniensis 05-110hOral swab930930Hyphal fringeRed to maroon
    C. dubliniensis 05-111hOral swab930930Hyphal fringeRed to maroon
    C. dubliniensis 05-112hOral swab930930Hyphal fringeRed to maroon
    C. dubliniensis 05-131hOral swab930930Hyphal fringeRed to maroon
    C. dubliniensis 05-139hOral swab930930Hyphal fringePale pink
    C. dubliniensis MAL CD1Oral swab930930Hyphal fringePale pink
    C. dubliniensis MAL CD2Oral swab930930Hyphal fringeRed to maroon
    C. dubliniensis MAL CD3Oral swab930930Hyphal fringeRed to maroon
    • ↵ a The reference strain C. albicans ATCC 10231 is from the American Type Culture Collection.

    • ↵ b The reference strain C. dubliniensis CBS 7987 is from the Centraalbureau voor Schimmelcultures.

    • ↵ c TTC, triphenyltetrazolium chloride (15).

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Amplification of the Hyphal Wall Protein 1 Gene To Distinguish Candida albicans from Candida dubliniensis
Orazio Romeo, Cosimo Racco, Giuseppe Criseo
Journal of Clinical Microbiology Jul 2006, 44 (7) 2590-2592; DOI: 10.1128/JCM.00125-06

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Amplification of the Hyphal Wall Protein 1 Gene To Distinguish Candida albicans from Candida dubliniensis
Orazio Romeo, Cosimo Racco, Giuseppe Criseo
Journal of Clinical Microbiology Jul 2006, 44 (7) 2590-2592; DOI: 10.1128/JCM.00125-06
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KEYWORDS

Candida
Fungal Proteins
Membrane Glycoproteins
mycology
polymerase chain reaction
Polymorphism, Restriction Fragment Length

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