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Journal of Clinical Microbiology
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Mycobacteriology and Aerobic Actinomycetes

Evaluation of Real-Time and Conventional PCR Targeting Complex 85 Genes for Detection of Mycobacterium leprae DNA in Skin Biopsy Samples from Patients Diagnosed with Leprosy

Alejandra N. Martinez, Constança F. P. C. Britto, José A. C. Nery, Elizabeth P. Sampaio, Márcia R. Jardim, Euzenir N. Sarno, Milton O. Moraes
Alejandra N. Martinez
1Leprosy Laboratory, Department of Mycobacterioses, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil
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Constança F. P. C. Britto
2Department of Biochemistry and Molecular Biology, Oswaldo Cruz Institute, Fiocruz, Av. Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, Brazil
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José A. C. Nery
1Leprosy Laboratory, Department of Mycobacterioses, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil
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Elizabeth P. Sampaio
1Leprosy Laboratory, Department of Mycobacterioses, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil
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Márcia R. Jardim
1Leprosy Laboratory, Department of Mycobacterioses, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil
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Euzenir N. Sarno
1Leprosy Laboratory, Department of Mycobacterioses, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil
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Milton O. Moraes
1Leprosy Laboratory, Department of Mycobacterioses, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil
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  • For correspondence: mmoraes@fiocruz.br
DOI: 10.1128/JCM.02250-05
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Article Information

DOI 
https://doi.org/10.1128/JCM.02250-05
PubMed 
16954241

Published By 
American Society for Microbiology Journals
History 
  • Received October 26, 2005
  • Returned for modification December 23, 2005
  • Accepted June 11, 2006
  • Published online September 5, 2006.

Copyright & Usage 
Copyright © 2006 American Society for Microbiology

Author Information

  1. Alejandra N. Martinez1,
  2. Constança F. P. C. Britto2,
  3. José A. C. Nery1,
  4. Elizabeth P. Sampaio1,
  5. Márcia R. Jardim1,
  6. Euzenir N. Sarno1 and
  7. Milton O. Moraes1*
  1. 1Leprosy Laboratory, Department of Mycobacterioses, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil
  2. 2Department of Biochemistry and Molecular Biology, Oswaldo Cruz Institute, Fiocruz, Av. Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, Brazil
  • ↵* Corresponding author. Mailing address: Leprosy Laboratory, Mycobacterioses Department, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, 21040-360, Brazil. Phone: (21) 25984467. Fax: (21) 22709997. E-mail: mmoraes{at}fiocruz.br .

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Evaluation of Real-Time and Conventional PCR Targeting Complex 85 Genes for Detection of Mycobacterium leprae DNA in Skin Biopsy Samples from Patients Diagnosed with Leprosy
Alejandra N. Martinez, Constança F. P. C. Britto, José A. C. Nery, Elizabeth P. Sampaio, Márcia R. Jardim, Euzenir N. Sarno, Milton O. Moraes
Journal of Clinical Microbiology Sep 2006, 44 (9) 3154-3159; DOI: 10.1128/JCM.02250-05

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Evaluation of Real-Time and Conventional PCR Targeting Complex 85 Genes for Detection of Mycobacterium leprae DNA in Skin Biopsy Samples from Patients Diagnosed with Leprosy
Alejandra N. Martinez, Constança F. P. C. Britto, José A. C. Nery, Elizabeth P. Sampaio, Márcia R. Jardim, Euzenir N. Sarno, Milton O. Moraes
Journal of Clinical Microbiology Sep 2006, 44 (9) 3154-3159; DOI: 10.1128/JCM.02250-05
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KEYWORDS

Antigens, Bacterial
leprosy
Mycobacterium leprae
polymerase chain reaction
skin

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