Loss of Catabolic Function in Streptococcus agalactiae Strains and Its Association with Neonatal Meningitis

Anne-Sophie Domelier; Nathalie van der Mee-Marquet; Adeline Grandet; Laurent Mereghetti; Agnès Rosenau; Roland Quentin

doi:10.1128/JCM.02550-05

DOI: 10.1128/JCM.02550-05

Article Figures & Data

Tables

TABLE 1.

Oxidation of 27 of 95 carbon sources in the Biolog system by 109 S. agalactiae strains^a

Position on microplate	Carbon source	No. of strains in indicated group oxidizing carbon source (%)			P value^d
Position on microplate	Carbon source	Total	HR group^b	LR group^c	P value^d
D1	β-Methyl-d-glucoside	66 (61)	31 (49)	35 (76)	0.004
D7	d-Ribose	47 (43)	15 (24)	32 (70)	<0.001
H10	α-d-Glucose-1-phosphate	28 (26)	6 (9)	22 (48)	<0.001
H12	d,l-α-Glycerol phosphate	67 (61)	30 (48)	37 (80)	<0.001
D8	Salicin	80 (73)	42 (67)	38 (83)	NS
B7	d-Galactose	85 (80)	46 (73)	39 (85)	NS
A8	Tween 40	1 (1)	0	1 (2)	NS
B3	Arbutin	10 (9)	3 (5)	7 (15)	NS
B4	Cellobiose	3 (3)	1 (2)	2 (4)	NS
C1	α-d-Lactose	8 (7)	5 (8)	3 (6)	NS
C2	Lactulose	7 (6)	4 (6)	3 (6)	NS
C7	d-Melezitose	1 (1)	0	1 (2)	NS
C8	d-Melibiose	1 (1)	0	1 (2)	NS
C9	α-Methyl-d-galactoside	1 (1)	0	1 (2)	NS
C10	β-Methyl-d-galactoside	1 (1)	0	1 (2)	NS
C11	3-Methyl-d-glucose	4 (4)	1 (2)	3 (6)	NS
C12	α-Methyl-d-glucose	8 (7)	2 (3)	6 (13)	NS
D3	Palatinose	8 (7)	2 (3)	6 (13)	NS
D6	l-Rhamnose	1 (1)	1 (2)	0	NS
F7	Succinic acid monomethyl ester	1 (1)	0	1 (2)	NS
F10	Succinamic acid	1 (1)	0	1 (2)	NS
G2	d-Alanine	1 (1)	1 (2)	0	NS
G6	l-Glutamic acid	2 (2)	2 (3)	0	NS
G11	2,3-Butanediol	3 (3)	2 (3)	1 (2)	NS
F5	l-Malic acid	100 (92)	58 (92)	42 (91)	NS
F9	Pyruvic acid	107 (98)	62 (98)	45 (98)	NS
H6	Adenosine-5′-monophosphate	108 (99)	62 (98)	46 (100)	NS

↵ a Findings for the HR group differed from those of the LR group. Four carbon sources significantly distinguished strains of the HR group from those of the LR group.
↵ b HR group: 63 strains with high risk of association with neonatal meningitis.
↵ c LR group: 46 strains with low risk of association with neonatal meningitis.
↵ d P value by chi-square test. NS, not significant.

TABLE 2.

Biotypes of S. agalactiae strains defined on the basis of oxidation of four carbon sources (D1, D7, H10, and H12)

Biotype	Oxidation of the following carbon source:				No. (%) of strains in:
Biotype	D1	D7	H10	H12	HR group^a	LR group^b	Recent CSF group^c
B1	−	−	−	−	27 (43)	2 (5)	26 (62)
B2	−	−	+	−	2 (3)	1 (2)	0
B3	+	−	−	−	3 (5)	1 (2)	0
B4	−	+	−	+	2 (3)	1 (2)	1 (3)
B5	+	−	−	+	14 (22)	4 (9)	6 (14)
B6	+	+	−	+	10 (16)	12 (26)	3 (7)
B7	+	−	+	+	2 (3)	3 (6)	0
B8	+	+	+	+	2 (3)	13 (28)	1 (2)
B9	−	+	+	−	0	1 (2)	0
B10	−	−	+	+	0	1 (2)	0
B11	−	+	+	+	0	2 (5)	0
B12	−	−	−	+	0	1 (2)	0
B13	+	−	+	−	0	1 (2)	0
B14	+	+	−	−	0	1 (2)	0
B15	−	+	−	−	1 (2)	2 (5)	5 (12)

↵ a HR group: 63 strains with high risk of association with neonatal meningitis.
↵ b LR group: 46 strains with low risk of association with neonatal meningitis.
↵ c Recent strains: 42 strains isolated between 2002 and 2005 from CSF (neonatal meningitis).

TABLE 3.

Distribution of three genetic markers (tRNA 1.2 at the 3′ end of rRNA operons, IS1548 in the hylB gene, and GBSi1 downstream from the scpB gene) in S. agalactiae strains belonging to HR and LR groups and various biotypes

Genetic marker(s)	No. of strains isolated between 1986 and 1990^a				No. of recent strains of biotypes^b
	Group		Biotypes		No. of recent strains of biotypes^b
	HR^c	LR^d	B1-B6	B7-B15	B1-B6	B7-B15
tRNA 1.2 or GBSi1 or IS1548	23	20	31	12	10	1
tRNA 1.2 and GBSi1	27	6	28	5	24	4
tRNA 1.2 and IS1548	0	0	0	0	1	0
tRNA 1.2 and IS1548 and GBSi1	2	0	2	0	0	0
IS1548 and GBSi1	2	0	2	0	0	0
Total for strains with:
At least one marker	54	26	63	17	35	5
No marker	9	20	16	13	1	1

↵ a One hundred nine strains isolated between 1986 and 1990 from CSF and vaginal and gastric fluids.
↵ b Recent strains: 42 strains isolated between 2002 and 2005 from CSF (neonatal meningitis).
↵ c HR group: 63 strains with high risk of association with neonatal meningitis.
↵ d LR group: 46 strains with low risk of association with neonatal meningitis.