Article Figures & Data
Figures
- FIG. 1.
FTIR spectra as measured from an S. aureus strain exhibiting the normal phenotype (NP-1) and from the isogenic S. aureus strain displaying the small-colony-variant phenotype (n-SCV-1). Prominent differences between the spectra of both phenotypes are marked.
- FIG. 2.
FTIR dendrograms derived from the spectra of an isogenic clone harvested from broth media consisting of a clinically derived, natural S. aureus SCV (n-SCV-1), its parent strain with normal phenotype (NP-1), the hemB mutant displaying the SCV phenotype (hemB-1), and the hemB-complemented mutant (c-hemB-1). Spectra were analyzed in triplicate (a to c). The isolates with normal phenotypes (NP-1 and c-hemB-1) were grouped in one cluster, which was clearly separated from that of the isolates exhibiting the SCV phenotype (n-SCV-1 and hemB-1).
- FIG. 3.
Tracing the switch of the SCV phenotype into the normal phenotype by FTIR in broth. The spectra of the clinically derived n-SCV-2 reverted during cultivation in brain heart infusion broth (rn-SCV-2) were grouped into the cluster comprising those isolates exhibiting the normal phenotype, i.e., the parent strain (NP-2) and the hemB-complemented mutant (c-hemB-2). For comparison, the spectra of the isogenic hemB mutant displaying the SCV phenotype (hemB-2) were included. Spectra were analyzed in triplicate (a to c).
- FIG. 4.
FTIR dendrogram clustering the spectra of two epidemiologically unrelated pairs consisting both of a normal parent strain (NP-1 and NP-2) and its isogenic hemB mutant exhibiting the SCV phenotype (hemB-1 and hemB-2). Spectra were analyzed at least in quadruplicate (a to e). Despite their different clonality, isolates with the normal phenotype were grouped in one cluster, whereas isolates with the SCV phenotype were grouped in a second cluster.
