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Bacteriology

Fourier-Transform Infrared Spectroscopic Analysis Is a Powerful Tool for Studying the Dynamic Changes in Staphylococcus aureus Small-Colony Variants

Karsten Becker, Nahed Al Laham, Wolfgang Fegeler, Richard A. Proctor, Georg Peters, Christof von Eiff
Karsten Becker
1Institute of Medical Microbiology, University Hospital of Münster, 48149 Münster, Germany
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  • For correspondence: kbecker@uni-muenster.de
Nahed Al Laham
1Institute of Medical Microbiology, University Hospital of Münster, 48149 Münster, Germany
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Wolfgang Fegeler
1Institute of Medical Microbiology, University Hospital of Münster, 48149 Münster, Germany
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Richard A. Proctor
2Department of Medical Microbiology/Immunology and Department of Medicine, University of Wisconsin Medical School, Madison, Wisconsin
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Georg Peters
1Institute of Medical Microbiology, University Hospital of Münster, 48149 Münster, Germany
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Christof von Eiff
1Institute of Medical Microbiology, University Hospital of Münster, 48149 Münster, Germany
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DOI: 10.1128/JCM.00847-06
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  • FIG. 1.
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    FIG. 1.

    FTIR spectra as measured from an S. aureus strain exhibiting the normal phenotype (NP-1) and from the isogenic S. aureus strain displaying the small-colony-variant phenotype (n-SCV-1). Prominent differences between the spectra of both phenotypes are marked.

  • FIG. 2.
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    FIG. 2.

    FTIR dendrograms derived from the spectra of an isogenic clone harvested from broth media consisting of a clinically derived, natural S. aureus SCV (n-SCV-1), its parent strain with normal phenotype (NP-1), the hemB mutant displaying the SCV phenotype (hemB-1), and the hemB-complemented mutant (c-hemB-1). Spectra were analyzed in triplicate (a to c). The isolates with normal phenotypes (NP-1 and c-hemB-1) were grouped in one cluster, which was clearly separated from that of the isolates exhibiting the SCV phenotype (n-SCV-1 and hemB-1).

  • FIG. 3.
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    FIG. 3.

    Tracing the switch of the SCV phenotype into the normal phenotype by FTIR in broth. The spectra of the clinically derived n-SCV-2 reverted during cultivation in brain heart infusion broth (rn-SCV-2) were grouped into the cluster comprising those isolates exhibiting the normal phenotype, i.e., the parent strain (NP-2) and the hemB-complemented mutant (c-hemB-2). For comparison, the spectra of the isogenic hemB mutant displaying the SCV phenotype (hemB-2) were included. Spectra were analyzed in triplicate (a to c).

  • FIG. 4.
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    FIG. 4.

    FTIR dendrogram clustering the spectra of two epidemiologically unrelated pairs consisting both of a normal parent strain (NP-1 and NP-2) and its isogenic hemB mutant exhibiting the SCV phenotype (hemB-1 and hemB-2). Spectra were analyzed at least in quadruplicate (a to e). Despite their different clonality, isolates with the normal phenotype were grouped in one cluster, whereas isolates with the SCV phenotype were grouped in a second cluster.

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Fourier-Transform Infrared Spectroscopic Analysis Is a Powerful Tool for Studying the Dynamic Changes in Staphylococcus aureus Small-Colony Variants
Karsten Becker, Nahed Al Laham, Wolfgang Fegeler, Richard A. Proctor, Georg Peters, Christof von Eiff
Journal of Clinical Microbiology Sep 2006, 44 (9) 3274-3278; DOI: 10.1128/JCM.00847-06

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Fourier-Transform Infrared Spectroscopic Analysis Is a Powerful Tool for Studying the Dynamic Changes in Staphylococcus aureus Small-Colony Variants
Karsten Becker, Nahed Al Laham, Wolfgang Fegeler, Richard A. Proctor, Georg Peters, Christof von Eiff
Journal of Clinical Microbiology Sep 2006, 44 (9) 3274-3278; DOI: 10.1128/JCM.00847-06
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KEYWORDS

Bacterial Typing Techniques
Spectroscopy, Fourier Transform Infrared
Staphylococcus aureus

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