Article Figures & Data
Figures
- FIG. 1.
Comparison of TcdC nucleotide and amino acid sequences to the published sequence for the reference strain VPI10463. Dots and dashes indicate identical bases and deletions, respectively. Only amino acid changes are depicted. Stop codons are underlined. Genotype tcdC-sc2 is not depicted but is identical to tcdC-sc1 with the exception of an inserted nucleotide (t) in the untranslated region at position 212.
- FIG. 2.
Minimum-spanning tree of MLVA data depicting genetic relatedness of 199 isolates of C. difficile from cases of CDAD at UPMC-P in 2001 and 2005. Each circle depicts one MLVA genotype, with the STRD between adjacent isolates displayed in gray. Where no numeral is shown, the STRD equals 1. STRDs of ≥11 are depicted with dashed lines. Clouds of gray surround isolates with an STRD of ≤2; these complexes represent closely related isolates. White circles depict genotypes represented by one isolate; light gray circles with black numerals, dark gray circles with white numerals, and black circles with white numerals depict genotypes represented by 2, 3, and ≥5 isolates, respectively. The circles are labeled with the tcdC genotype described in Fig. 1 (wild-type tcdC = 0, tcdC-A = A, tcdC-B = B, tcdC-sc1 = 1, tcdC-sc2 = 2, tcdC-sc3 = 3, etc.); available REA types are displayed below the tcdC genotype. The tree has been redrawn for ease of viewing and is not to scale.
Tables
- TABLE 1.
Characteristics of tcdC genotypes of isolates from 199 cases of CDAD at UPMC 2001 and 2005
tcdC genotypea No. of cases (%) Predicted truncating mutations Nucleotide deletions (>1 bp) Deletion (>1 bp) beyond stop codon? Predicted TcdC length (amino acid residues) No. of residue changes in TcdC (no. deleted) REA type(s) within group (no. typed) Wild type (VPI10463) 36 (18.1) 232 0 (0) K (1) tcdC-A 3 (1.5) C184T 39 Yes 61 2 (171) tcdC-B 9 (4.5) None 18 No 226 0 (6) tcdC-sc1 91 (45.7) Δ117A 18 Yes 65 27 (167) BI 9 (3), BI10 (2) tcdC-sc2* 1 (0.5) Δ117A 18 Yes 65 27 (167) BR2 (1) tcdC-sc3 23 (11.9) None None 232 1 (0) J9, 28-31 (6) tcdC-sc4 1 (0.5) None 18 No 226 1 (6) tcdC-sc5 5 (2.5) None None 232 2 (0) tcdC-sc7 6 (3.0) None None 232 2 (0) tcdC-sc8 1 (0.5) Δ117A None 65 26 (167) tcdC-sc9 9 (4.5) None None 232 1 (0) CL (1) tcdC-sc10 1 (0.5) None None 232 2 (0) tcdC-sc12 2 (1.0) None None 232 2 (0) tcdC-sc13 1 (0.5) None None 232 0 (0) tcdC-sc14 3 (1.5) None None 232 1 (0) BM (1) tcdC-sc15 5 (2.5) None None 232 0 (0) tcdC-sc16 1 (0.5) C191A 36 Yes 63 2 (169) tcdC-sc17 1 (0.5) G258A 18 Yes 85 0 (147) ↵ a *, Genotype tcdC-sc2 is identical to genotype tcdC-sc1 except for the insertion of a single nucleotide (t) at position 212.
